ID   MED1_DICDI              Reviewed;         724 AA.
AC   Q54SI1;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 1;
DE   AltName: Full=Putative mediator complex subunit 1;
GN   Name=med1; ORFNames=DDB_G0282453;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 1 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000047; EAL66086.1; -; Genomic_DNA.
DR   RefSeq; XP_640058.1; XM_634966.1.
DR   AlphaFoldDB; Q54SI1; -.
DR   STRING; 44689.DDB0266834; -.
DR   PaxDb; Q54SI1; -.
DR   EnsemblProtists; EAL66086; EAL66086; DDB_G0282453.
DR   GeneID; 8623585; -.
DR   KEGG; ddi:DDB_G0282453; -.
DR   dictyBase; DDB_G0282453; med1.
DR   eggNOG; ENOG502SF7Q; Eukaryota.
DR   HOGENOM; CLU_382400_0_0_1; -.
DR   InParanoid; Q54SI1; -.
DR   OMA; QTIKFTC; -.
DR   PRO; PR:Q54SI1; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0016592; C:mediator complex; ISS:dictyBase.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:UniProt.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019680; Mediator_Med1.
DR   Pfam; PF10744; Med1; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..724
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 1"
FT                   /id="PRO_0000379419"
FT   REGION          33..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          147..167
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          486..524
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          140..200
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   724 AA;  83687 MW;  60DA7BA74CA92273 CRC64;
     MDHHKQLTLQ TTLESCNNLY NLLFNSIGSI KPSQQQQQQQ QNQKNTNSVT SNTNIINDQE
     IFKLNKFHLY GENNIDELYN SIELQLQTIK FTCQQYRQWD STERDSIKFF NTQKLYNQSQ
     QRGNLVSKTL FDCSLLGKIY LNQQKEQQQQ QKQKNKQQQQ QPQPLVEDLS KNISKTVEVK
     RKLGLLAGKL QEMTNELNNK YSTFSVLDDL LGLLKSNTEI DYNSDSYVSC NISSSTFLLD
     IDIYHNGEIK EVKLVHILTT TGEVEPAEQQ FNDELTNSLK TDMKEFIKKV QRICDLDLLF
     RKYKHFDLQK AFSILQSDFL NISINSNIKY IDNKEIEMNK GFGEIKLDCC GVLIKYFQSY
     IEKISKMNEP YSIMIEMESS AVTNNGSLID QSEYSRLSLK TLLQKQQHTQ PSAQTDYSEQ
     QQQQSISTQL TSIEFNEKDC LDCEPLSSML ESDSIFSPVR LVCKLNKPIL ITNQQLSKIL
     NLSKIHRSSQ QPSQQQPSQQ QQQQQQQQQQ KKLNSVDESM NENGDSNIIE NINELIKKYS
     IQNLLISSSS SSSSSNDSNI SVNVDNSFDC EVYGMKQRYY YTGEFELGIE ISRIPIYHPS
     QVYPTIQLLR QQIVFNILFK SCFQNLNISK SDDHHNIIDN NNDVKIFEIT SNPPNSINII
     FLHPIDNSFN SIDIFIKNNG DLEAFYYDNT TNIQPNIQKS TLFTKMLIKS LSISVSLACF
     FKNK