MED1_DROME
ID MED1_DROME Reviewed; 1475 AA.
AC Q9VP05; B5RJH3; Q9GYW7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 1;
DE AltName: Full=Mediator complex subunit 1;
DE AltName: Full=dTRAP220;
GN Name=MED1; Synonyms=Trap220; ORFNames=CG7162;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Southworth J.W., Kennison J.A.;
RT "Transcriptional coactivators in Drosophila.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-830; SER-834; SER-854 AND
RP SER-858, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC activated transcription of the MtnA, MtnB and MtnD genes. {ECO:0000250,
CC ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 1 family.
CC {ECO:0000305}.
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DR EMBL; AF289996; AAG02461.1; -; mRNA.
DR EMBL; AE014296; AAF51757.2; -; Genomic_DNA.
DR EMBL; BT044447; ACH92512.1; -; mRNA.
DR RefSeq; NP_649341.1; NM_141084.3.
DR AlphaFoldDB; Q9VP05; -.
DR BioGRID; 65647; 11.
DR IntAct; Q9VP05; 3.
DR STRING; 7227.FBpp0078118; -.
DR iPTMnet; Q9VP05; -.
DR PaxDb; Q9VP05; -.
DR PRIDE; Q9VP05; -.
DR DNASU; 40403; -.
DR EnsemblMetazoa; FBtr0078464; FBpp0078118; FBgn0037109.
DR GeneID; 40403; -.
DR KEGG; dme:Dmel_CG7162; -.
DR CTD; 5469; -.
DR FlyBase; FBgn0037109; MED1.
DR VEuPathDB; VectorBase:FBgn0037109; -.
DR eggNOG; ENOG502QPZ7; Eukaryota.
DR GeneTree; ENSGT00660000095569; -.
DR InParanoid; Q9VP05; -.
DR OMA; HAMKLTY; -.
DR OrthoDB; 182447at2759; -.
DR PhylomeDB; Q9VP05; -.
DR Reactome; R-DME-383280; Nuclear Receptor transcription pathway.
DR Reactome; R-DME-400206; Regulation of lipid metabolism by PPARalpha.
DR Reactome; R-DME-9018519; Estrogen-dependent gene expression.
DR Reactome; R-DME-9707564; Cytoprotection by HMOX1.
DR SignaLink; Q9VP05; -.
DR BioGRID-ORCS; 40403; 0 hits in 1 CRISPR screen.
DR ChiTaRS; MED1; fly.
DR GenomeRNAi; 40403; -.
DR PRO; PR:Q9VP05; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0037109; Expressed in cleaving embryo and 22 other tissues.
DR ExpressionAtlas; Q9VP05; baseline and differential.
DR Genevisible; Q9VP05; DM.
DR GO; GO:0016592; C:mediator complex; IMP:FlyBase.
DR GO; GO:0005634; C:nucleus; IC:FlyBase.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR InterPro; IPR019680; Mediator_Med1.
DR Pfam; PF10744; Med1; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1475
FT /note="Mediator of RNA polymerase II transcription subunit
FT 1"
FT /id="PRO_0000302023"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 616..644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 709..992
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1135..1166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1184..1245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1263..1354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1387..1475
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 616..642
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 709..730
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 753..782
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 783..801
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 856..936
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 956..990
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1184..1232
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1263..1299
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1311..1345
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1387..1426
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1444..1460
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 830
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 834
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 854
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 858
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 1475 AA; 149531 MW; 273E6CB3949DC6CE CRC64;
MSGSNAKSSG TGFGSHIPSI EEKNKQIQQE TMMEKLRAKY RNKPKSYEEI KKSVRMYFIE
KHYPLDPLCK ATLQSALDKL QHYIKVTSRH GLVERLESLS RQLGLKFMED QQLLFISTDM
FYVEILLDAA GSLSDVKVHH ECKIEQQSSE LVACLKSGDF ADFTVQLEGL SSIYQLNAEP
KVKKKAFVAL QAMETDIQSL YQLHLQGHSG DSYSLMTSSS VGLVLPRRGG HPMRLTYFCP
PLHLPEGDPK LASGDFTIDQ VMRSSYGLSA TINLEGSSAN KLQTLPTVTL VRDAQTGLEV
PTYAQLNQNN SLLMPATFVL RLNKPMPVCL ESLKALGLPG LDSVATPPGP PTTVLNLIVQ
TASKQAIKNT QRGLYVNLPK ETHCYFFTDN RKLQGTLVSS LPFTEPAQVP RIVAFLKKQA
LFYTLLASCV REQQKQYNDM DSTVILEVTA VSFNQITVEL QHPYEESLAT VDFLLEDGQP
TCSVYCLTNE YELLSQKLTR TARKVVSIPM VIYKLLKCWD EEHEFKLHGA IGPGSGSGAI
GGGGMSGGGP VSGVGNNFSQ FSMDTPTPSD GSLPGGGFAN INNLKMDAKS RSLADAFAAS
TSAAAAIAGL INLKRETDPQ SGSSASGTTV SGSSSSSGSA KTSDHDIADK YKNIWKDKTP
NLKHCVSITP IPGDGKSGSA GGVSGVEVQR TGGIEIIPLN AQAAIAGGGV TASSSATPTT
ITITPITGKD PSKDSTKKST AASAGVGVAA KRPHESSTSS SSTSGCSGSG SSMSSSASSG
SSDTQKEKKR KKKRDDSPMG PPEKIYSRQN SPAGGADASA TGGVVRKFSS PSSSPKAGGG
GQGLMAGVPT ARPSPKHSPV YSSPKHNTAS NSPKSPFGTH SPKHGSSGKP SMSTLKSAAT
AATILSPKGD KSSSAVGNTS SGPSASSGSS GATGLVRSFA SVGAPPPPPP IPPLASSSGS
ISSSQSLKKE KTSSASGSSS TSSSATAGVA SGGGISPASV AAAVAALKSS QQQMKSVASL
SHLAAGGGLG SYAAPSGAGA SGAAAVVVGA GAGAGAGASG LELSALRKGM AGGAVSLMTS
TAALAPTIPA PTTTVAAGSA ASLVSPVSAV VGQGQETAGA AAAATLATAT ILQQQPQPGA
APTSSCLTTS GGSSDSAGSI NPAGASTEYM VKPSSQEGLK LTINKTGSSK SSGTGSGSSS
SSGLQAKAKS SSSGATSFAG STGSTKKQHT GLKPGVNSGP ASKKATAAVS SATASSSKHF
FQKANSSGNL SSKLSGSGSG GGIPLTKSNS TNSFQEHNAP RRRPSMGALA SGSSGGGSGQ
RKLGSASGGG SGSSGSVSPA LSGSMSQPPP RFDHHTDMMT ILQYASPTMA ASMEGFIKGL
HNKFQIPKLS QRGSGGNTTS GRSTPSGSSE PALAGTSSSI LGPIASSTGL TEPEAKPPVP
PSQSGNEGLL NLSSTAGTPS ADGIDEELLA SLAGE
