ID   MED1_KLULA              Reviewed;         426 AA.
AC   Q6CVU5;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 1;
DE   AltName: Full=Mediator complex subunit 1;
GN   Name=MED1; OrderedLocusNames=KLLA0B09350g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 1 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382122; CAH02337.1; -; Genomic_DNA.
DR   RefSeq; XP_451944.1; XM_451944.1.
DR   AlphaFoldDB; Q6CVU5; -.
DR   STRING; 28985.XP_451944.1; -.
DR   EnsemblFungi; CAH02337; CAH02337; KLLA0_B09350g.
DR   GeneID; 2896927; -.
DR   KEGG; kla:KLLA0_B09350g; -.
DR   eggNOG; ENOG502QW0Y; Eukaryota.
DR   HOGENOM; CLU_021764_0_0_1; -.
DR   InParanoid; Q6CVU5; -.
DR   OMA; NDKFGIY; -.
DR   Proteomes; UP000000598; Chromosome B.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR019680; Mediator_Med1.
DR   Pfam; PF10744; Med1; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..426
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   1"
FT                   /id="PRO_0000302030"
FT   REGION          319..349
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        319..347
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   426 AA;  49159 MW;  B015924922349CCA CRC64;
     MSDAFVELLD EMIERLLNYK PGSRTLPNVI KLCQTLGLES FVDQVDSTKS RLSIASNIVV
     IDIDYENEME TILDVKLVLA SNFDKFNYFN EKGENILLTS LSDVQDLKAF HHNLNFLVFL
     DSFSNIDIES GHTSLDLFKY YTDLPKMLQD YISDQHLPFT VKMNENSTFG VSIYDAQGQT
     KIMTVDLEKA PNSDRSFYEY VYDSKLKDWL NESSDASTQG INLVFKFEEL VAFPETWLTT
     EIQMNETPKK FELPQQSHLK HTVKLQNELT SDLLLMDSFR ISNEDIALLP DFLKWYNWHK
     IVLQEVLKLI IHDNVTSSIS TSNSGSVQPK PRRKSSVLSN RRPSMTDSMM LRDSGIPEFT
     LKEILDQPVI SDTEDDDQMD VDKEKQIPIV LNEEYIYIGK AQTCSYNDND EQAWKAFIDY
     LKSKLI