ID   MED1_SCHPO              Reviewed;         454 AA.
AC   Q09696;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 1;
DE   AltName: Full=Mediator complex subunit 1;
DE   AltName: Full=RNA polymerase II Mediator complex protein pmc2;
GN   Name=med1; Synonyms=pmc2; ORFNames=SPAC2F7.04;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP   COMPLEX.
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=10625684; DOI=10.1074/jbc.275.2.1351;
RA   Spaehr H., Beve J., Larsson T., Bergstroem J., Karlsson K.-A.,
RA   Gustafsson C.M.;
RT   "Purification and characterization of RNA polymerase II holoenzyme from
RT   Schizosaccharomyces pombe.";
RL   J. Biol. Chem. 275:1351-1356(2000).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP   COMPLEX.
RX   PubMed=11572939; DOI=10.1073/pnas.211253898;
RA   Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA   Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT   "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT   subunits conserved between yeast and metazoan cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors.
CC   -!- SUBUNIT: Component of the Mediator complex.
CC       {ECO:0000269|PubMed:10625684, ECO:0000269|PubMed:11572939}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 1 family.
CC       {ECO:0000305}.
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DR   EMBL; CU329670; CAA90491.1; -; Genomic_DNA.
DR   PIR; T38552; S58148.
DR   RefSeq; NP_592975.1; NM_001018375.2.
DR   AlphaFoldDB; Q09696; -.
DR   BioGRID; 278974; 94.
DR   IntAct; Q09696; 1.
DR   STRING; 4896.SPAC2F7.04.1; -.
DR   SwissPalm; Q09696; -.
DR   MaxQB; Q09696; -.
DR   PaxDb; Q09696; -.
DR   EnsemblFungi; SPAC2F7.04.1; SPAC2F7.04.1:pep; SPAC2F7.04.
DR   GeneID; 2542516; -.
DR   KEGG; spo:SPAC2F7.04; -.
DR   PomBase; SPAC2F7.04; med1.
DR   VEuPathDB; FungiDB:SPAC2F7.04; -.
DR   eggNOG; ENOG502RYK5; Eukaryota.
DR   HOGENOM; CLU_602903_0_0_1; -.
DR   InParanoid; Q09696; -.
DR   OMA; QQSCFQY; -.
DR   PRO; PR:Q09696; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0003713; F:transcription coactivator activity; IDA:PomBase.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; EXP:PomBase.
DR   InterPro; IPR019680; Mediator_Med1.
DR   Pfam; PF10744; Med1; 2.
PE   1: Evidence at protein level;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..454
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   1"
FT                   /id="PRO_0000096371"
SQ   SEQUENCE   454 AA;  51390 MW;  C6366431E1E6758C CRC64;
     MDRPHLFSEV HFRRNASNPN QINWTRYWID CLSSVPWNDI SVTGFEYLAK KYGLEVFQDS
     SKPNEVVLSL AGKIILIDIT VPINASPKDI NVVLAFANAS GEQYNNPVAE KLLKDAIYNC
     NTPLFEKNVK WLATFDHSSP SVQQSCFQYL DSLSSSLNAI YEAELSLLAQ EENVIMHGNG
     KPLSNYEGQL GLRIVYWKLL DKTYSTQIFM DNLSHESLPH LLFGYNLLNS PPILQSSKIN
     WALENTLTPI PTTMELVFDD INLIIPEQGV KPLLDLLHVH DITIPWPVQN YSHMLGLPSK
     STVNYKFINK NQAIQLTGFD VSARSLRHVP FHHPKQIRGI LAIVRQYLLL QLILENIKSA
     DLAETAASSS LHLSLYFKEH PIVHAQYQEI NKLNNSEQII IVLSVLSDGR LNVDYMSSGG
     KELSKQQSHV FQKLIQQTCN IGLAIEVFIK KVVN