ID   MED1_XENTR              Reviewed;        1573 AA.
AC   A1L0Z0;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 1;
DE   AltName: Full=Mediator complex subunit 1;
GN   Name=med1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity).
CC       {ECO:0000250|UniProtKB:Q15648}.
CC   -!- SUBUNIT: Component of the Mediator complex.
CC       {ECO:0000250|UniProtKB:Q15648}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q15648}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 1 family.
CC       {ECO:0000305}.
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DR   EMBL; BC127309; AAI27310.1; -; mRNA.
DR   RefSeq; NP_001090727.1; NM_001097258.1.
DR   PDB; 2ZMH; X-ray; 2.10 A; C=624-636.
DR   PDB; 2ZMI; X-ray; 1.70 A; C=624-636.
DR   PDB; 2ZMJ; X-ray; 2.35 A; C=624-636.
DR   PDB; 2ZXM; X-ray; 3.01 A; C=624-636.
DR   PDB; 2ZXN; X-ray; 2.10 A; C=624-636.
DR   PDB; 3AFR; X-ray; 2.00 A; C=624-636.
DR   PDBsum; 2ZMH; -.
DR   PDBsum; 2ZMI; -.
DR   PDBsum; 2ZMJ; -.
DR   PDBsum; 2ZXM; -.
DR   PDBsum; 2ZXN; -.
DR   PDBsum; 3AFR; -.
DR   AlphaFoldDB; A1L0Z0; -.
DR   SMR; A1L0Z0; -.
DR   STRING; 8364.ENSXETP00000051133; -.
DR   PaxDb; A1L0Z0; -.
DR   GeneID; 100036710; -.
DR   KEGG; xtr:100036710; -.
DR   CTD; 5469; -.
DR   eggNOG; ENOG502QPZ7; Eukaryota.
DR   InParanoid; A1L0Z0; -.
DR   OrthoDB; 57581at2759; -.
DR   EvolutionaryTrace; A1L0Z0; -.
DR   Proteomes; UP000008143; Chromosome 10.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:UniProt.
DR   IDEAL; IID50031; -.
DR   InterPro; IPR019680; Mediator_Med1.
DR   Pfam; PF10744; Med1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1573
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   1"
FT                   /id="PRO_0000302021"
FT   REGION          595..691
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          774..883
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          928..1564
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           588..592
FT                   /note="LXXLL motif 1"
FT   MOTIF           629..633
FT                   /note="LXXLL motif 2"
FT   COMPBIAS        636..665
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        788..807
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        834..871
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        930..945
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        987..1013
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1014..1044
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1062..1114
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1120..1197
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1214..1298
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1347..1364
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1421..1490
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1508..1523
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           627..633
FT                   /evidence="ECO:0007829|PDB:2ZMI"
SQ   SEQUENCE   1573 AA;  165656 MW;  6386F10B9E8862E8 CRC64;
     MSSLLERLHS KYSQNRPWPE TIKLVRQIME KRTGMMSGSH QHLVSCLETL QKALKVSSLS
     AMTDRLESIA RQNGLTSHLS PNGTECYITT DMFYLEVLLD TEGQLCDVKV AHHRENPVSC
     PELVEQLREK NFEDFSQHLK GLVNLYKVPG DNKLETKMYL ALQSLELDLT KMAAMYWQAT
     NASVLEKILH GTVGYLTPRS GGQVMSLKYY VSPYDLFDDT TGASISLSEG HAVPRSLGMN
     VSVTIEATSA MYKLPIAPLI MGSHPIDNKG TPSFTSITNA NSVDLPACFF LKFPQPIPVS
     RAFIQKIQHC TGIPLIDGSH TFLPHYELVT QFELAKEKEP GTLNHNMRFY ASLPGQQHCY
     FVNKDAPLPD GRSLQGTLLS KIPFQHPGRV PIILSLIRHQ VACNTLIGSC VKRTMLKEDC
     PGLLQFEVAP LSDSCFSISF QHPVNDSLVC VVMDVQDSSH VSCKLYKGLS DALICTDDFI
     TKVVQRCMSI PVTMRAIRRK AETIQADTPA LSLIAETVED MVKKNLPPAS SPGYGMASGS
     TALSGITTPT SSYTSGQISS LFNMGIKERH DSAGHGDDFS KVTQNPILTS LLQITSNSGG
     TLGSSPTPPQ HTPPPASSPA SNTKNHPMLM NLLKDNPAQD FSTLYGGSPL ERQNSSGSPR
     TELGSSASGK PKKKRPRTGT EKMKNQTEDD FQRELFSMDV DSQNTIFDVG MAGDALDTPH
     ITPAPSQCGT PPNVYQQAIP HTQANMQRMV RIPSTDAIIP DVTDILSDIA EEASKLPGPG
     EDCPNLGTPV RDSSSSGHSQ STLFDTDVFQ VDGGGGSGGE NPYPDPVDLI VDSHGSPNSD
     SPNTFFNSVD FNPDLLNSQS QSGFTDDLND DSSQSGDNDF KDFAGPGLAS LGIVSGLPVD
     GGDGKYKMAL GADTVDFSII AGGGSKNLGG PDIQETQSRS QSPLLGSDLG KDRPQKQKVK
     ESGNGGGAGG GLSGMQSAGM EGKSMKRSRT PSSDGKSKDK PPKRKKTESD GKSPSHITNR
     PFTPPTSTGG SKSPGTSGRS QTPPGMATPP IPKITIQIPK GTMSVGKPSS HSQYSSSGSS
     SSSSSKSHHG HSSLSSSASG KIKSNKSDGS SGMKIGSSGG GMYSGQSGQS NSQSKNSSQS
     MGKAGSSPIT KHGLSSNVNN SSGSKTKPQG KPSVLMNPSL SKPNISPSHS RPSGGSDKLS
     SPMKPMPGTP PSSKAKSPIG SGGQHLSGSG SNSTTKSSSG LVSSGSLTQK PNSSSSSSSS
     SSSSSSSSSS SSSSFSSGVS QNLHSSSKGK SPSRNKKPSL TAVIDKLKHG VGTGGPGSED
     PMDGGVGGGS TGAPSHGMSS KHGLGVGEFP TKREKGEKDK SKGSSGGPSD PSKKGGGDSK
     GSVSTGVAKI IISKHDGGSP SIKAKVTLQK PEGGGEGLRS QMQKNYGSPL ISGSTPKHER
     CSPSHNKSPA YTPQALDSES ESGSSSIAEK SYQNSPSSDD GGGSGSRAQT EYSAEKHKKH
     KKEKKRLKDK DRDREKKKSY TMKPESWSKS PISADPTMAM GGGAMMSSDR ASRPTPSFLM
     GDDDDLIDVP LTL