ID   MED20_DICDI             Reviewed;         195 AA.
AC   Q54FL3;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 20;
DE   AltName: Full=Mediator complex subunit 20;
GN   Name=med20; ORFNames=DDB_G0290781;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 20 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000171; EAL62024.1; -; Genomic_DNA.
DR   RefSeq; XP_635527.1; XM_630435.1.
DR   AlphaFoldDB; Q54FL3; -.
DR   SMR; Q54FL3; -.
DR   STRING; 44689.DDB0266923; -.
DR   PaxDb; Q54FL3; -.
DR   EnsemblProtists; EAL62024; EAL62024; DDB_G0290781.
DR   GeneID; 8627824; -.
DR   KEGG; ddi:DDB_G0290781; -.
DR   dictyBase; DDB_G0290781; med20.
DR   eggNOG; KOG1383; Eukaryota.
DR   HOGENOM; CLU_1398668_0_0_1; -.
DR   InParanoid; Q54FL3; -.
DR   OMA; VKWLMHW; -.
DR   PhylomeDB; Q54FL3; -.
DR   PRO; PR:Q54FL3; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; ISS:dictyBase.
DR   InterPro; IPR013921; Mediator_Med20.
DR   PANTHER; PTHR12465; PTHR12465; 1.
DR   Pfam; PF08612; Med20; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..195
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 20"
FT                   /id="PRO_0000328900"
SQ   SEQUENCE   195 AA;  22316 MW;  898FCA6DB8E88815 CRC64;
     MGYKCIFSFK GQLPEMTKRI EFLGGIKVST GTINCSLYLE RVPDGSNLIP REFHLLLYDE
     KPKKCFTVCR DTIVETDREM VSILEKTNSY KKRQTTEALI SKYELGDFII RVGPIVFRTE
     IRGILVEVEY TACSTPVTSP FNCTKLLSEF INGNLGPFPE PIQTSFDFSA YPTLPKSYSD
     LHTSYQYVNL FRTIN