MED20_DROME
ID MED20_DROME Reviewed; 252 AA.
AC P91641; Q9VLT4;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 20;
DE AltName: Full=Mediator complex subunit 20;
DE AltName: Full=Protein trf-proximal;
DE AltName: Full=dMED20;
DE AltName: Full=dTrap26;
DE AltName: Full=dTrfp;
GN Name=MED20; Synonyms=Tmr, Trfp; ORFNames=CG18780;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF52599.2};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RA Crowley T.E.;
RT "Mutations near the Trf cluster cause a premeiotic defect in the Drosophila
RT male germ line.";
RL Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION OF THE MEDIATOR COMPLEX,
RP IDENTIFICATION IN A COMPLEX WITH CDK8; MED4; MED6; MED14; MED17; MED18;
RP MED21 AND MED31, AND DEVELOPMENTAL STAGE.
RX PubMed=11259581; DOI=10.1128/mcb.21.7.2312-2323.2001;
RA Park J.M., Gim B.S., Kim J.M., Yoon J.H., Kim H.-S., Kang J.-G., Kim Y.-J.;
RT "Drosophila Mediator complex is broadly utilized by diverse gene-specific
RT transcription factors at different types of core promoters.";
RL Mol. Cell. Biol. 21:2312-2323(2001).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND FUNCTION OF THE MEDIATOR COMPLEX.
RX PubMed=12021283; DOI=10.1074/jbc.m204144200;
RA Gu J.-Y., Park J.M., Song E.J., Mizuguchi G., Yoon J.H., Kim-Ha J.,
RA Lee K.-J., Kim Y.-J.;
RT "Novel Mediator proteins of the small Mediator complex in Drosophila SL2
RT cells.";
RL J. Biol. Chem. 277:27154-27161(2002).
RN [7]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. Required for activated
CC transcription of the MtnA gene. {ECO:0000269|PubMed:11259581,
CC ECO:0000269|PubMed:12021283, ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex, which includes at least
CC CDK8, MED4, MED6, MED11, MED14, MED17, MED18, MED20, MED21, MED22,
CC MED27, MED28, MED30 and MED31. {ECO:0000269|PubMed:11259581,
CC ECO:0000269|PubMed:12021283}.
CC -!- INTERACTION:
CC P91641; Q9VEC1: MED17; NbExp=6; IntAct=EBI-175591, EBI-135284;
CC P91641; Q9XZT1: MED18; NbExp=4; IntAct=EBI-175591, EBI-148856;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Maternally encoded. Expression decreases during
CC larval stages then rises during mid-pupal metamorphosis.
CC {ECO:0000269|PubMed:11259581}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 20 family.
CC {ECO:0000305}.
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DR EMBL; Y10975; CAA71871.1; -; mRNA.
DR EMBL; AE014134; AAF52599.2; -; Genomic_DNA.
DR EMBL; BT003473; AAO39476.1; -; mRNA.
DR RefSeq; NP_524663.2; NM_079924.3.
DR AlphaFoldDB; P91641; -.
DR SMR; P91641; -.
DR BioGRID; 68741; 34.
DR DIP; DIP-21881N; -.
DR IntAct; P91641; 4.
DR STRING; 7227.FBpp0079166; -.
DR PaxDb; P91641; -.
DR DNASU; 43952; -.
DR EnsemblMetazoa; FBtr0079544; FBpp0079166; FBgn0013531.
DR GeneID; 43952; -.
DR KEGG; dme:Dmel_CG18780; -.
DR CTD; 9477; -.
DR FlyBase; FBgn0013531; MED20.
DR VEuPathDB; VectorBase:FBgn0013531; -.
DR eggNOG; KOG4309; Eukaryota.
DR GeneTree; ENSGT00390000002060; -.
DR HOGENOM; CLU_080044_1_0_1; -.
DR InParanoid; P91641; -.
DR OMA; NDIYEPM; -.
DR OrthoDB; 1112080at2759; -.
DR PhylomeDB; P91641; -.
DR BioGRID-ORCS; 43952; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43952; -.
DR PRO; PR:P91641; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0013531; Expressed in secondary oocyte and 20 other tissues.
DR ExpressionAtlas; P91641; baseline and differential.
DR Genevisible; P91641; DM.
DR GO; GO:0016592; C:mediator complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:GOC.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR InterPro; IPR013921; Mediator_Med20.
DR PANTHER; PTHR12465; PTHR12465; 1.
DR Pfam; PF08612; Med20; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..252
FT /note="Mediator of RNA polymerase II transcription subunit
FT 20"
FT /id="PRO_0000096373"
FT CONFLICT 220
FT /note="H -> Q (in Ref. 1; CAA71871)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 252 AA; 27862 MW; 608AB5A43DAC52F5 CRC64;
MGVTILQPYP LPEGKSGAHI IDQLSKRLLA LGATHAGQFL VDCETFISTP QPHNGAPGRA
VHVLHNSEYP ASTFSIIDNG TGKQVAIVAD NIFDLLMLKM TNTFTSKKQT KIESRGARFE
YGDFVIKLGS VTMMEHFKGI LIEIEYKSCV ILAYCWEMIR EMLQGFLGIA VNKDFPSYFA
PQTIMTAMGQ QQLHAKHNDI FEPMDTVKQY LEQFTNYRKH VTLMGGMGSG PGSQQVGPNV
HMSPAVAGLH RS
