ID   MED20_KLULA             Reviewed;         211 AA.
AC   Q6CTQ9;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 20;
DE   AltName: Full=Mediator complex subunit 20;
GN   Name=SRB2; Synonyms=MED20; OrderedLocusNames=KLLA0C10780g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 20 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382123; CAH01531.1; -; Genomic_DNA.
DR   RefSeq; XP_452680.1; XM_452680.1.
DR   AlphaFoldDB; Q6CTQ9; -.
DR   SMR; Q6CTQ9; -.
DR   STRING; 28985.XP_452680.1; -.
DR   EnsemblFungi; CAH01531; CAH01531; KLLA0_C10780g.
DR   GeneID; 2891888; -.
DR   KEGG; kla:KLLA0_C10780g; -.
DR   eggNOG; ENOG502RXMU; Eukaryota.
DR   HOGENOM; CLU_065844_1_0_1; -.
DR   InParanoid; Q6CTQ9; -.
DR   OMA; QRQSIKG; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0019904; F:protein domain specific binding; IEA:EnsemblFungi.
DR   GO; GO:0001094; F:TFIID-class transcription factor complex binding; IEA:EnsemblFungi.
DR   GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR   GO; GO:0010689; P:negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus; IEA:EnsemblFungi.
DR   GO; GO:0010691; P:negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR016532; Med20.
DR   InterPro; IPR013921; Mediator_Med20.
DR   Pfam; PF08612; Med20; 1.
DR   PIRSF; PIRSF007945; SRB2; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..211
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   20"
FT                   /id="PRO_0000308566"
SQ   SEQUENCE   211 AA;  23605 MW;  DEFAF863EC465F81 CRC64;
     MTYYGVLFVE KCTPSTITQL QDVLSTDLIS MGDKWSFELK TFRTSAKNTD PNDTKVMHTV
     QLSHKNNETV TIKNQSAIIT PTYVTKALYD NGCVFGTPEP FDYMLSNKLS NIWTQRQSIK
     GEFGVSYQTA DLSIRVNNAF SYSGFQGLIL ELESKSSDNL EAFEKNVERV RTMLSGMGLS
     DVRVSSDKSQ GSKQEDSSLF DLAAHYLKVL G