ID   MED21_DICDI             Reviewed;         197 AA.
AC   Q54HN4;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 21;
DE   AltName: Full=Putative mediator complex subunit 21;
GN   Name=med21; ORFNames=DDB_G0289339;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 21 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000139; EAL62756.1; -; Genomic_DNA.
DR   RefSeq; XP_636267.1; XM_631175.1.
DR   AlphaFoldDB; Q54HN4; -.
DR   SMR; Q54HN4; -.
DR   STRING; 44689.DDB0266929; -.
DR   PaxDb; Q54HN4; -.
DR   EnsemblProtists; EAL62756; EAL62756; DDB_G0289339.
DR   GeneID; 8627086; -.
DR   KEGG; ddi:DDB_G0289339; -.
DR   dictyBase; DDB_G0289339; med21.
DR   eggNOG; ENOG502RI3X; Eukaryota.
DR   HOGENOM; CLU_1386431_0_0_1; -.
DR   InParanoid; Q54HN4; -.
DR   OMA; YVFGTCI; -.
DR   PRO; PR:Q54HN4; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0016592; C:mediator complex; ISS:dictyBase.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:dictyBase.
DR   InterPro; IPR037212; Med7/Med21-like.
DR   InterPro; IPR021384; Mediator_Med21.
DR   PANTHER; PTHR13381; PTHR13381; 1.
DR   Pfam; PF11221; Med21; 1.
DR   SUPFAM; SSF140718; SSF140718; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..197
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 21"
FT                   /id="PRO_0000388658"
FT   REGION          29..110
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          38..101
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        31..65
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        66..80
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        81..107
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   197 AA;  23229 MW;  2A467F0365F7AAB9 CRC64;
     MDLITQLQDK LDHLLYVFGT CIGVLQRDAP PSFFNNPQNQ QQQQQQQQQQ QQQQQQQQQQ
     QNPQTQQQLP PPPPPPPQQQ QQQQQQQQQQ QQQQQQQQQQ QPQPTEEWDA PSKMALQVIE
     TSKVIESYIE KLPGFDKTED QQYEDLKNLN TQSKQVSNEL LSSRRDAIEL LKMVKESILY
     ISEESKNEEI DQQPMQQ