MED21_DROME
ID MED21_DROME Reviewed; 142 AA.
AC Q9W5P1; A8Y534;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 21;
DE AltName: Full=Mediator complex subunit 21;
DE AltName: Full=dMED21;
DE AltName: Full=dSRB7;
DE AltName: Full=dTRAP19;
GN Name=MED21; Synonyms=Trap19; ORFNames=CG17397;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION OF THE MEDIATOR COMPLEX, IDENTIFICATION IN A COMPLEX WITH CDK8;
RP MED4; MED6; MED14; MED17; MED18; MED20 AND MED31, AND DEVELOPMENTAL STAGE.
RX PubMed=11259581; DOI=10.1128/mcb.21.7.2312-2323.2001;
RA Park J.M., Gim B.S., Kim J.M., Yoon J.H., Kim H.-S., Kang J.-G., Kim Y.-J.;
RT "Drosophila Mediator complex is broadly utilized by diverse gene-specific
RT transcription factors at different types of core promoters.";
RL Mol. Cell. Biol. 21:2312-2323(2001).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND INTERACTION WITH MED6 AND MED17.
RX PubMed=12021283; DOI=10.1074/jbc.m204144200;
RA Gu J.-Y., Park J.M., Song E.J., Mizuguchi G., Yoon J.H., Kim-Ha J.,
RA Lee K.-J., Kim Y.-J.;
RT "Novel Mediator proteins of the small Mediator complex in Drosophila SL2
RT cells.";
RL J. Biol. Chem. 277:27154-27161(2002).
RN [6]
RP INTERACTION WITH MED4; MED7 AND MED10.
RX PubMed=15477388; DOI=10.1093/nar/gkh878;
RA Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M.,
RA Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M.;
RT "A high resolution protein interaction map of the yeast Mediator complex.";
RL Nucleic Acids Res. 32:5379-5391(2004).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. Required for activated
CC transcription of the MtnA, MtnB and MtnD genes.
CC {ECO:0000269|PubMed:11259581}.
CC -!- SUBUNIT: Component of the Mediator complex, which includes at least
CC MED4, MED6, MED14, MED17, MED18, MED20, MED21, MED23, MED24, MED27,
CC MED30 and MED31. Also interacts with MED7 and MED10.
CC {ECO:0000269|PubMed:11259581, ECO:0000269|PubMed:12021283,
CC ECO:0000269|PubMed:15477388}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Maternally encoded. Expression decreases during
CC larval stages then rises during mid-pupal metamorphosis.
CC {ECO:0000269|PubMed:11259581}.
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DR EMBL; AE014296; EAL24605.1; -; Genomic_DNA.
DR EMBL; AE014296; EAL24606.1; -; Genomic_DNA.
DR EMBL; AY071027; AAL48649.1; -; mRNA.
DR RefSeq; NP_001015223.1; NM_001015223.3.
DR RefSeq; NP_001015224.1; NM_001015224.3.
DR AlphaFoldDB; Q9W5P1; -.
DR SMR; Q9W5P1; -.
DR BioGRID; 78102; 7.
DR IntAct; Q9W5P1; 6.
DR STRING; 7227.FBpp0112462; -.
DR PaxDb; Q9W5P1; -.
DR DNASU; 3354977; -.
DR EnsemblMetazoa; FBtr0113739; FBpp0112462; FBgn0040020.
DR EnsemblMetazoa; FBtr0113740; FBpp0112463; FBgn0040020.
DR GeneID; 3354977; -.
DR KEGG; dme:Dmel_CG17397; -.
DR CTD; 9412; -.
DR FlyBase; FBgn0040020; MED21.
DR VEuPathDB; VectorBase:FBgn0040020; -.
DR eggNOG; KOG1510; Eukaryota.
DR GeneTree; ENSGT00390000014557; -.
DR HOGENOM; CLU_126757_0_0_1; -.
DR InParanoid; Q9W5P1; -.
DR OMA; QAENFCN; -.
DR OrthoDB; 1583203at2759; -.
DR PhylomeDB; Q9W5P1; -.
DR SignaLink; Q9W5P1; -.
DR BioGRID-ORCS; 3354977; 1 hit in 1 CRISPR screen.
DR ChiTaRS; MED21; fly.
DR GenomeRNAi; 3354977; -.
DR PRO; PR:Q9W5P1; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0040020; Expressed in cleaving embryo and 26 other tissues.
DR ExpressionAtlas; Q9W5P1; baseline and differential.
DR Genevisible; Q9W5P1; DM.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:GOC.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR InterPro; IPR037212; Med7/Med21-like.
DR InterPro; IPR021384; Mediator_Med21.
DR PANTHER; PTHR13381; PTHR13381; 1.
DR Pfam; PF11221; Med21; 1.
DR SUPFAM; SSF140718; SSF140718; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..142
FT /note="Mediator of RNA polymerase II transcription subunit
FT 21"
FT /id="PRO_0000305954"
FT COILED 87..111
FT /evidence="ECO:0000255"
SQ SEQUENCE 142 AA; 16069 MW; 95E2BDF375322DAC CRC64;
MADRLTQLQD TVNQQAEHFC NAIGVIQQTS LPSKFVNFER IGPQTPIPCP PQEDYAQLFA
QLIARCAKDI DTLIESLPNE DSSIELQNSS LKRLEIENQG TARDLEEVVQ KGELLLEKMQ
YSLECIAQAQ LDMQITLKHD LQ
