MED21_MOUSE
ID MED21_MOUSE Reviewed; 144 AA.
AC Q9CQ39; Q921I5;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 21;
DE AltName: Full=Mediator complex subunit 21;
DE AltName: Full=RNA polymerase II holoenzyme component SRB7;
DE Short=RNAPII complex component SRB7;
GN Name=Med21; Synonyms=Srb7, Surb7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, Head, Placenta, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=10500093; DOI=10.1101/gad.13.18.2365;
RA Tudor M., Murray P.J., Onufryk C., Jaenisch R., Young R.A.;
RT "Ubiquitous expression and embryonic requirement for RNA polymerase II
RT coactivator subunit Srb7 in mice.";
RL Genes Dev. 13:2365-2368(1999).
RN [4]
RP INTERACTION WITH PPARG.
RX PubMed=12037571; DOI=10.1038/417563a;
RA Ge K., Guermah M., Yuan C.-X., Ito M., Wallberg A.E., Spiegelman B.M.,
RA Roeder R.G.;
RT "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated
RT adipogenesis.";
RL Nature 417:563-567(2002).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with THRA in a
CC ligand-dependent fashion (By similarity). Interacts with PPARG.
CC {ECO:0000250, ECO:0000269|PubMed:12037571}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in brain, heart, kidney, liver, lung,
CC skeletal muscles, spleen and testis. {ECO:0000269|PubMed:10500093}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 21 family.
CC {ECO:0000305}.
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DR EMBL; AK009742; BAB26472.1; -; mRNA.
DR EMBL; AK012217; BAB28104.1; -; mRNA.
DR EMBL; AK014428; BAB29343.1; -; mRNA.
DR EMBL; AK019417; BAB31711.1; -; mRNA.
DR EMBL; BC012286; AAH12286.1; -; mRNA.
DR CCDS; CCDS20700.1; -.
DR RefSeq; NP_079591.1; NM_025315.3.
DR PDB; 6W1S; EM; 4.02 A; P=3-128.
DR PDBsum; 6W1S; -.
DR AlphaFoldDB; Q9CQ39; -.
DR SMR; Q9CQ39; -.
DR BioGRID; 223831; 9.
DR ComplexPortal; CPX-3264; Core mediator complex.
DR CORUM; Q9CQ39; -.
DR DIP; DIP-59522N; -.
DR IntAct; Q9CQ39; 6.
DR MINT; Q9CQ39; -.
DR STRING; 10090.ENSMUSP00000032429; -.
DR iPTMnet; Q9CQ39; -.
DR PhosphoSitePlus; Q9CQ39; -.
DR EPD; Q9CQ39; -.
DR MaxQB; Q9CQ39; -.
DR PaxDb; Q9CQ39; -.
DR PeptideAtlas; Q9CQ39; -.
DR PRIDE; Q9CQ39; -.
DR ProteomicsDB; 295861; -.
DR Antibodypedia; 1803; 169 antibodies from 25 providers.
DR DNASU; 108098; -.
DR Ensembl; ENSMUST00000032429; ENSMUSP00000032429; ENSMUSG00000030291.
DR Ensembl; ENSMUST00000204040; ENSMUSP00000145512; ENSMUSG00000030291.
DR GeneID; 108098; -.
DR KEGG; mmu:108098; -.
DR UCSC; uc009esg.1; mouse.
DR CTD; 9412; -.
DR MGI; MGI:1347064; Med21.
DR VEuPathDB; HostDB:ENSMUSG00000030291; -.
DR eggNOG; KOG1510; Eukaryota.
DR GeneTree; ENSGT00390000014557; -.
DR HOGENOM; CLU_126757_0_0_1; -.
DR InParanoid; Q9CQ39; -.
DR OMA; QAENFCN; -.
DR OrthoDB; 1583203at2759; -.
DR PhylomeDB; Q9CQ39; -.
DR BioGRID-ORCS; 108098; 22 hits in 75 CRISPR screens.
DR ChiTaRS; Med21; mouse.
DR PRO; PR:Q9CQ39; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9CQ39; protein.
DR Bgee; ENSMUSG00000030291; Expressed in animal zygote and 249 other tissues.
DR ExpressionAtlas; Q9CQ39; baseline and differential.
DR Genevisible; Q9CQ39; MM.
DR GO; GO:0070847; C:core mediator complex; ISO:MGI.
DR GO; GO:0016592; C:mediator complex; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0000151; C:ubiquitin ligase complex; ISO:MGI.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:MGI.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISO:MGI.
DR GO; GO:0001824; P:blastocyst development; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0016567; P:protein ubiquitination; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR GO; GO:0035019; P:somatic stem cell population maintenance; IMP:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IC:MGI.
DR InterPro; IPR037212; Med7/Med21-like.
DR InterPro; IPR021384; Mediator_Med21.
DR PANTHER; PTHR13381; PTHR13381; 1.
DR Pfam; PF11221; Med21; 1.
DR SUPFAM; SSF140718; SSF140718; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..144
FT /note="Mediator of RNA polymerase II transcription subunit
FT 21"
FT /id="PRO_0000305947"
FT CONFLICT 137
FT /note="H -> R (in Ref. 2; AAH12286)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 144 AA; 15588 MW; CCFEA1DF0B6B53AC CRC64;
MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFSNIQT AINKDQPANP TEEYAQLFAA
LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA ATCLEDVVYR GDMLLEKIQS
ALADIAQSQL KTRSVTHSHS LPDS
