MED21_SCHPO
ID MED21_SCHPO Reviewed; 138 AA.
AC O94376;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 21;
DE AltName: Full=Mediator complex subunit 21;
DE AltName: Full=Suppressor of RNA polymerase B 7;
GN Name=med21; Synonyms=srb7; ORFNames=SPBC1604.10;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RX PubMed=11572939; DOI=10.1073/pnas.211253898;
RA Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT subunits conserved between yeast and metazoan cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:11572939}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 21 family.
CC {ECO:0000305}.
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DR EMBL; CU329671; CAA22343.1; -; Genomic_DNA.
DR PIR; T39504; T39504.
DR RefSeq; NP_596630.1; NM_001022551.2.
DR PDB; 5N9J; X-ray; 3.40 A; D=1-138.
DR PDB; 5U0P; EM; 4.40 A; U=1-138.
DR PDB; 5U0S; EM; 7.80 A; U=1-138.
DR PDBsum; 5N9J; -.
DR PDBsum; 5U0P; -.
DR PDBsum; 5U0S; -.
DR AlphaFoldDB; O94376; -.
DR SMR; O94376; -.
DR BioGRID; 276742; 10.
DR IntAct; O94376; 1.
DR STRING; 4896.SPBC1604.10.1; -.
DR MaxQB; O94376; -.
DR PaxDb; O94376; -.
DR EnsemblFungi; SPBC1604.10.1; SPBC1604.10.1:pep; SPBC1604.10.
DR GeneID; 2540209; -.
DR KEGG; spo:SPBC1604.10; -.
DR PomBase; SPBC1604.10; -.
DR VEuPathDB; FungiDB:SPBC1604.10; -.
DR eggNOG; KOG1510; Eukaryota.
DR HOGENOM; CLU_094271_1_1_1; -.
DR InParanoid; O94376; -.
DR OMA; DKNHDFE; -.
DR PhylomeDB; O94376; -.
DR PRO; PR:O94376; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:PomBase.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:PomBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR037212; Med7/Med21-like.
DR InterPro; IPR021384; Mediator_Med21.
DR PANTHER; PTHR13381; PTHR13381; 1.
DR Pfam; PF11221; Med21; 1.
DR SUPFAM; SSF140718; SSF140718; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Coiled coil; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..138
FT /note="Mediator of RNA polymerase II transcription subunit
FT 21"
FT /id="PRO_0000096376"
FT COILED 87..128
FT /evidence="ECO:0000255"
FT HELIX 4..28
FT /evidence="ECO:0007829|PDB:5N9J"
FT STRAND 35..37
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 43..46
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 51..78
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 87..136
FT /evidence="ECO:0007829|PDB:5N9J"
SQ SEQUENCE 138 AA; 15800 MW; 5A7C236108C09AF9 CRC64;
MACRCTQLQD TIDEVATQFY SSIHYLSSHH DFVPLPGQEK VSDSKVNPIS AEELQFAQRD
LAKDLVTKFM QIDTLINQLP GISTAPKHQL EKIKKLQNSI EEKQLERKSL ESENEDLKLQ
LAKRIETFGR LSCVLFQP
