ID   MED22_BOVIN             Reviewed;         201 AA.
AC   Q5E9K2; A6QM07;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 22;
DE   AltName: Full=Mediator complex subunit 22;
DE   AltName: Full=Surfeit locus protein 5;
GN   Name=MED22; Synonyms=SURF5;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1] {ECO:0000312|EMBL:AAX08935.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=Hereford; TISSUE=Thymus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC       MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC       MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC       MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC       CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC       module termed the CDK8 module. Mediator containing the CDK8 module is
CC       less active than Mediator lacking this module in supporting
CC       transcriptional activation. Individual preparations of the Mediator
CC       complex lacking one or more distinct subunits have been variously
CC       termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=Surf5B;
CC         IsoId=Q5E9K2-1; Sequence=Displayed;
CC       Name=2; Synonyms=Surf5A;
CC         IsoId=Q5E9K2-2; Sequence=VSP_028991, VSP_028992;
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BT020918; AAX08935.1; -; mRNA.
DR   EMBL; BC148153; AAI48154.1; -; mRNA.
DR   RefSeq; NP_001029914.1; NM_001034742.1. [Q5E9K2-1]
DR   RefSeq; XP_005213417.1; XM_005213360.3. [Q5E9K2-1]
DR   RefSeq; XP_015329142.1; XM_015473656.1.
DR   AlphaFoldDB; Q5E9K2; -.
DR   SMR; Q5E9K2; -.
DR   STRING; 9913.ENSBTAP00000015357; -.
DR   PaxDb; Q5E9K2; -.
DR   PRIDE; Q5E9K2; -.
DR   Ensembl; ENSBTAT00000015357; ENSBTAP00000015357; ENSBTAG00000011556. [Q5E9K2-1]
DR   GeneID; 613736; -.
DR   KEGG; bta:613736; -.
DR   CTD; 6837; -.
DR   VEuPathDB; HostDB:ENSBTAG00000011556; -.
DR   eggNOG; KOG3304; Eukaryota.
DR   GeneTree; ENSGT00390000004339; -.
DR   HOGENOM; CLU_117242_0_0_1; -.
DR   InParanoid; Q5E9K2; -.
DR   OMA; TQCEQDT; -.
DR   OrthoDB; 1573728at2759; -.
DR   TreeFam; TF323390; -.
DR   Proteomes; UP000009136; Chromosome 11.
DR   Bgee; ENSBTAG00000011556; Expressed in laryngeal cartilage and 104 other tissues.
DR   GO; GO:0070847; C:core mediator complex; IEA:Ensembl.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR009332; Med22.
DR   PANTHER; PTHR12434; PTHR12434; 1.
DR   Pfam; PF06179; Med22; 1.
PE   2: Evidence at transcript level;
KW   Activator; Alternative splicing; Coiled coil; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..201
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   22"
FT                   /id="PRO_0000278121"
FT   REGION          166..201
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          93..123
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         138..140
FT                   /note="SSS -> RYK (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_028991"
FT   VAR_SEQ         141..201
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_028992"
SQ   SEQUENCE   201 AA;  22381 MW;  477D4242E3A84684 CRC64;
     MAQQRALPQS KETLLQSYNK RLKDDVKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE
     MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ RNQQLRALQE ECDRKLIALR
     DEVSIDLYEL EEEYYSSSSS LCEANDLPLC EAYWRLDPDT DSTDCLSAPL LASPEPSAGG
     PLQAAAPTHS HAGGPGPTEH A