MED22_DANRE
ID MED22_DANRE Reviewed; 198 AA.
AC A5WUL3; Q6IQK8;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 22;
DE AltName: Full=Mediator complex subunit 22;
DE AltName: Full=Surfeit locus protein 5;
GN Name=med22; Synonyms=surf5; ORFNames=si:ch73-278c20.1, si:dkey-226m8.2;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=A5WUL3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A5WUL3-2; Sequence=VSP_028997, VSP_028998;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 22 family.
CC {ECO:0000305}.
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DR EMBL; BX927184; CAN88486.1; -; Genomic_DNA.
DR EMBL; CU019641; CAN88486.1; JOINED; Genomic_DNA.
DR EMBL; CR382323; CAN88767.1; -; Genomic_DNA.
DR EMBL; CR382323; CAN88768.1; -; Genomic_DNA.
DR EMBL; BC071397; AAH71397.1; -; mRNA.
DR RefSeq; NP_001002144.1; NM_001002144.1. [A5WUL3-2]
DR AlphaFoldDB; A5WUL3; -.
DR SMR; A5WUL3; -.
DR STRING; 7955.ENSDARP00000111825; -.
DR PaxDb; A5WUL3; -.
DR PeptideAtlas; A5WUL3; -.
DR Ensembl; ENSDART00000125093; ENSDARP00000109672; ENSDARG00000014148. [A5WUL3-2]
DR Ensembl; ENSDART00000146759; ENSDARP00000113772; ENSDARG00000014148. [A5WUL3-2]
DR GeneID; 415234; -.
DR KEGG; dre:415234; -.
DR CTD; 6837; -.
DR ZFIN; ZDB-GENE-040625-156; med22.
DR eggNOG; KOG3304; Eukaryota.
DR GeneTree; ENSGT00390000004339; -.
DR HOGENOM; CLU_117242_0_0_1; -.
DR InParanoid; A5WUL3; -.
DR OMA; TQCEQDT; -.
DR PhylomeDB; A5WUL3; -.
DR TreeFam; TF323390; -.
DR PRO; PR:A5WUL3; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 5.
DR Bgee; ENSDARG00000014148; Expressed in swim bladder and 27 other tissues.
DR ExpressionAtlas; A5WUL3; baseline.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR InterPro; IPR009332; Med22.
DR PANTHER; PTHR12434; PTHR12434; 1.
DR Pfam; PF06179; Med22; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..198
FT /note="Mediator of RNA polymerase II transcription subunit
FT 22"
FT /id="PRO_0000308569"
FT REGION 159..198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 170..184
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 138..140
FT /note="SCG -> RYK (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_028997"
FT VAR_SEQ 141..198
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_028998"
SQ SEQUENCE 198 AA; 22681 MW; 81CF04AB77110A0D CRC64;
MSTPRVLPQS KETLLQNYNK RLKDDIRSIL DNFTEIIKTA KVEDETQVSR ATQAEQDHFE
MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAISL RNQQLRTLQE ECDKKLISLR
DEIAIDLYEL EEEYYSSSCG QWDGVELPLC EAFHRQDSWG SPEMTSDPSH ANHEVSDHLG
SQESMQRHRN GSGTSEQS
