MED22_MOUSE
ID MED22_MOUSE Reviewed; 200 AA.
AC Q62276; A2ALA2; Q3TTZ9;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 22;
DE AltName: Full=Mediator complex subunit 22;
DE AltName: Full=Surfeit locus protein 5;
DE Short=Surf-5;
GN Name=Med22; Synonyms=Surf5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BALB/cJ;
RX PubMed=8586415; DOI=10.1006/geno.1995.9889;
RA Garson K., Duhig T., Armes N., Colombo P., Fried M.;
RT "Surf5: a gene in the tightly clustered mouse surfeit locus is highly
RT conserved and transcribed divergently from the rpL7A (Surf3) gene.";
RL Genomics 30:163-170(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Lung, Mammary gland, and Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=Surf5B;
CC IsoId=Q62276-1; Sequence=Displayed;
CC Name=2; Synonyms=Surf5A;
CC IsoId=Q62276-2; Sequence=VSP_028993, VSP_028994;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 22 family.
CC {ECO:0000305}.
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DR EMBL; X85169; CAA59453.1; -; Genomic_DNA.
DR EMBL; AK144744; BAE26044.1; -; mRNA.
DR EMBL; AK161053; BAE36173.1; -; mRNA.
DR EMBL; AK166250; BAE38661.1; -; mRNA.
DR EMBL; AK166474; BAE38795.1; -; mRNA.
DR EMBL; AL773563; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC018225; AAH18225.1; -; mRNA.
DR CCDS; CCDS15813.1; -. [Q62276-1]
DR CCDS; CCDS15814.1; -. [Q62276-2]
DR RefSeq; NP_001029080.1; NM_001033908.1. [Q62276-1]
DR RefSeq; NP_035643.1; NM_011513.2. [Q62276-2]
DR PDB; 6W1S; EM; 4.02 A; Q=9-139.
DR PDBsum; 6W1S; -.
DR AlphaFoldDB; Q62276; -.
DR SMR; Q62276; -.
DR BioGRID; 203582; 34.
DR ComplexPortal; CPX-3264; Core mediator complex.
DR IntAct; Q62276; 33.
DR STRING; 10090.ENSMUSP00000015920; -.
DR iPTMnet; Q62276; -.
DR PhosphoSitePlus; Q62276; -.
DR EPD; Q62276; -.
DR MaxQB; Q62276; -.
DR PaxDb; Q62276; -.
DR PRIDE; Q62276; -.
DR ProteomicsDB; 295862; -. [Q62276-1]
DR ProteomicsDB; 295863; -. [Q62276-2]
DR Antibodypedia; 18330; 178 antibodies from 26 providers.
DR DNASU; 20933; -.
DR Ensembl; ENSMUST00000015920; ENSMUSP00000015920; ENSMUSG00000015776. [Q62276-1]
DR Ensembl; ENSMUST00000102899; ENSMUSP00000099963; ENSMUSG00000015776. [Q62276-2]
DR GeneID; 20933; -.
DR KEGG; mmu:20933; -.
DR UCSC; uc008iwd.1; mouse. [Q62276-1]
DR UCSC; uc008iwe.1; mouse. [Q62276-2]
DR CTD; 6837; -.
DR MGI; MGI:98446; Med22.
DR VEuPathDB; HostDB:ENSMUSG00000015776; -.
DR eggNOG; KOG3304; Eukaryota.
DR GeneTree; ENSGT00390000004339; -.
DR HOGENOM; CLU_117242_0_0_1; -.
DR InParanoid; Q62276; -.
DR OMA; TQCEQDT; -.
DR OrthoDB; 1573728at2759; -.
DR PhylomeDB; Q62276; -.
DR TreeFam; TF323390; -.
DR BioGRID-ORCS; 20933; 22 hits in 73 CRISPR screens.
DR ChiTaRS; Med22; mouse.
DR PRO; PR:Q62276; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q62276; protein.
DR Bgee; ENSMUSG00000015776; Expressed in embryonic brain and 253 other tissues.
DR ExpressionAtlas; Q62276; baseline and differential.
DR Genevisible; Q62276; MM.
DR GO; GO:0070847; C:core mediator complex; ISO:MGI.
DR GO; GO:0016592; C:mediator complex; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR InterPro; IPR009332; Med22.
DR PANTHER; PTHR12434; PTHR12434; 1.
DR Pfam; PF06179; Med22; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative splicing; Coiled coil; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..200
FT /note="Mediator of RNA polymerase II transcription subunit
FT 22"
FT /id="PRO_0000178839"
FT REGION 169..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 93..122
FT /evidence="ECO:0000255"
FT VAR_SEQ 138..140
FT /note="SSS -> RYK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_028993"
FT VAR_SEQ 141..200
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_028994"
SQ SEQUENCE 200 AA; 22282 MW; AF3802A645AE5FC2 CRC64;
MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE
MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ RNQQLRALQE ECDRKLITLR
DEVSIDLYEL EEEYYSSSSS LCEANDLPLC EAYWRLDLDA DSADGLSAPL LASPETGAGP
LQSAAPVHSH GGGPGPTEHT
