ID   MED22_RAT               Reviewed;         200 AA.
AC   A0JPN6;
DT   20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 22;
DE   AltName: Full=Mediator complex subunit 22;
DE   AltName: Full=Surfeit locus protein 5;
GN   Name=Med22; Synonyms=Surf5;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1] {ECO:0000312|EMBL:AAI27520.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Heart {ECO:0000312|EMBL:AAI27520.1};
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC       MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC       MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC       MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC       CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC       module termed the CDK8 module. Mediator containing the CDK8 module is
CC       less active than Mediator lacking this module in supporting
CC       transcriptional activation. Individual preparations of the Mediator
CC       complex lacking one or more distinct subunits have been variously
CC       termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=Surf5B;
CC         IsoId=A0JPN6-1; Sequence=Displayed;
CC       Name=2; Synonyms=Surf5A;
CC         IsoId=A0JPN6-2; Sequence=VSP_028995, VSP_028996;
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 22 family.
CC       {ECO:0000305}.
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DR   EMBL; BC127519; AAI27520.1; -; mRNA.
DR   EMBL; DN932977; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; NP_001071147.1; NM_001077679.1. [A0JPN6-1]
DR   RefSeq; XP_003749478.1; XM_003749430.3. [A0JPN6-1]
DR   AlphaFoldDB; A0JPN6; -.
DR   SMR; A0JPN6; -.
DR   STRING; 10116.ENSRNOP00000006720; -.
DR   PaxDb; A0JPN6; -.
DR   PeptideAtlas; A0JPN6; -.
DR   Ensembl; ENSRNOT00000006720; ENSRNOP00000006720; ENSRNOG00000005059. [A0JPN6-1]
DR   GeneID; 499762; -.
DR   KEGG; rno:499762; -.
DR   UCSC; RGD:1564893; rat. [A0JPN6-1]
DR   CTD; 6837; -.
DR   RGD; 1564893; Med22.
DR   eggNOG; KOG3304; Eukaryota.
DR   GeneTree; ENSGT00390000004339; -.
DR   HOGENOM; CLU_117242_0_0_1; -.
DR   InParanoid; A0JPN6; -.
DR   OMA; TQCEQDT; -.
DR   OrthoDB; 1573728at2759; -.
DR   PhylomeDB; A0JPN6; -.
DR   TreeFam; TF323390; -.
DR   PRO; PR:A0JPN6; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   Bgee; ENSRNOG00000005059; Expressed in cerebellum and 19 other tissues.
DR   Genevisible; A0JPN6; RN.
DR   GO; GO:0070847; C:core mediator complex; ISO:RGD.
DR   GO; GO:0016592; C:mediator complex; ISO:RGD.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR009332; Med22.
DR   PANTHER; PTHR12434; PTHR12434; 1.
DR   Pfam; PF06179; Med22; 1.
PE   2: Evidence at transcript level;
KW   Activator; Alternative splicing; Coiled coil; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..200
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   22"
FT                   /id="PRO_0000278122"
FT   REGION          167..200
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          93..122
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         138..140
FT                   /note="SSS -> RYK (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_028995"
FT   VAR_SEQ         141..200
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_028996"
SQ   SEQUENCE   200 AA;  22312 MW;  BE3802B745AE5FC2 CRC64;
     MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE
     MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ RNQQLRALQE ECDRKLITLR
     DEVSIDLYEL EEEYYSSSSS LCEANDLPLC EAYWRLDLDT DSADGLSAPL LASPETGAGP
     LQSAAPVHSH GGGPGPTEHT