MED22_SCHPO
ID MED22_SCHPO Reviewed; 136 AA.
AC O14010;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 22;
DE AltName: Full=Mediator complex subunit 22;
DE AltName: Full=Suppressor of RNA polymerase B 6 homolog;
GN Name=med22; Synonyms=srb6; ORFNames=SPAC29A4.07;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RX PubMed=11572939; DOI=10.1073/pnas.211253898;
RA Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT subunits conserved between yeast and metazoan cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:11572939}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 22 family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAB10134.1; -; Genomic_DNA.
DR PIR; T38482; T38482.
DR RefSeq; NP_594875.1; NM_001020304.2.
DR PDB; 4H63; X-ray; 3.40 A; V=2-136.
DR PDB; 5N9J; X-ray; 3.40 A; Z=1-136.
DR PDB; 5U0P; EM; 4.40 A; V=1-136.
DR PDB; 5U0S; EM; 7.80 A; V=1-136.
DR PDBsum; 4H63; -.
DR PDBsum; 5N9J; -.
DR PDBsum; 5U0P; -.
DR PDBsum; 5U0S; -.
DR AlphaFoldDB; O14010; -.
DR SMR; O14010; -.
DR BioGRID; 278877; 3.
DR DIP; DIP-38756N; -.
DR IntAct; O14010; 3.
DR STRING; 4896.SPAC29A4.07.1; -.
DR MaxQB; O14010; -.
DR PaxDb; O14010; -.
DR EnsemblFungi; SPAC29A4.07.1; SPAC29A4.07.1:pep; SPAC29A4.07.
DR GeneID; 2542413; -.
DR KEGG; spo:SPAC29A4.07; -.
DR PomBase; SPAC29A4.07; -.
DR VEuPathDB; FungiDB:SPAC29A4.07; -.
DR eggNOG; ENOG502SD63; Eukaryota.
DR HOGENOM; CLU_1866296_0_0_1; -.
DR InParanoid; O14010; -.
DR OMA; YQIECHT; -.
DR PRO; PR:O14010; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:PomBase.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:PomBase.
DR InterPro; IPR009332; Med22.
DR PANTHER; PTHR12434; PTHR12434; 1.
DR Pfam; PF06179; Med22; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..136
FT /note="Mediator of RNA polymerase II transcription subunit
FT 22"
FT /id="PRO_0000096378"
FT HELIX 6..38
FT /evidence="ECO:0007829|PDB:4H63"
FT HELIX 46..83
FT /evidence="ECO:0007829|PDB:4H63"
FT HELIX 100..111
FT /evidence="ECO:0007829|PDB:4H63"
FT TURN 112..114
FT /evidence="ECO:0007829|PDB:4H63"
SQ SEQUENCE 136 AA; 15382 MW; 52EA5A74C06C9FE1 CRC64;
MSSDSFQRQL VQRTNTLNSS IDNATLTILS RFQDILDIAI NEGKDKYTVA PEVYQIECHT
VSMVRAVEQL LDVSRQIKSY WLTNSLSTSF PTVDYSEPDL EKVKRTLTKL QNHLLEVSLI
EPEASETTEA PTVSDT
