ID   MED22_TAKRU             Reviewed;         200 AA.
AC   O57595; O73882;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1999, sequence version 2.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 22;
DE   AltName: Full=Mediator complex subunit 22;
DE   AltName: Full=Surfeit locus protein 5;
GN   Name=med22; Synonyms=surf5;
OS   Takifugu rubripes (Japanese pufferfish) (Fugu rubripes).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Eupercaria; Tetraodontiformes; Tetradontoidea; Tetraodontidae; Takifugu.
OX   NCBI_TaxID=31033;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RX   PubMed=9414319; DOI=10.1101/gr.7.12.1138;
RA   Armes N., Gilley J., Fried M.;
RT   "The comparative genomic structure and sequence of the surfeit gene
RT   homologs in the puffer fish Fugu rubripes and their association with CpG-
RT   rich islands.";
RL   Genome Res. 7:1138-1152(1997).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Surf5B;
CC         IsoId=O57595-1; Sequence=Displayed;
CC       Name=Surf5A;
CC         IsoId=O57595-2; Sequence=VSP_006310;
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 22 family.
CC       {ECO:0000305}.
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DR   EMBL; Y15172; CAA75447.1; -; Genomic_DNA.
DR   EMBL; Y15172; CAA75448.1; -; Genomic_DNA.
DR   AlphaFoldDB; O57595; -.
DR   SMR; O57595; -.
DR   STRING; 31033.ENSTRUP00000007223; -.
DR   eggNOG; KOG3304; Eukaryota.
DR   InParanoid; O57595; -.
DR   Proteomes; UP000005226; Unplaced.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR009332; Med22.
DR   PANTHER; PTHR12434; PTHR12434; 1.
DR   Pfam; PF06179; Med22; 1.
PE   3: Inferred from homology;
KW   Activator; Alternative splicing; Coiled coil; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..200
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   22"
FT                   /id="PRO_0000178840"
FT   REGION          159..200
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          93..123
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        159..176
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        183..200
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         138..200
FT                   /note="SYSQWDTDLPLCEAYHRVDNWASPSSSSSSTQGDQEEVEILPSQETEPQHHL
FT                   NGQGTSSLEKM -> RYK (in isoform Surf5A)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_006310"
SQ   SEQUENCE   200 AA;  22923 MW;  09558487C91504D2 CRC64;
     MATQRVLPQS KETLLQSYNK RLKDDIKSIL ENFTEIIKTA KIEDETQVSR PAQAEQDHYE
     MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNDAISL QNQQLRSLQE ECDKKLISLR
     DEIAIDLYEL EEEYYSSSYS QWDTDLPLCE AYHRVDNWAS PSSSSSSTQG DQEEVEILPS
     QETEPQHHLN GQGTSSLEKM