MED23_ARATH
ID MED23_ARATH Reviewed; 1615 AA.
AC F4I4P3; F4I4P4; O49295; Q9LR38;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 23;
GN Name=MED23; Synonyms=MED23_1; OrderedLocusNames=At1g23230;
GN ORFNames=F26F24.8, T26J12.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [4]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4I4P3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4I4P3-2; Sequence=VSP_043991, VSP_043992;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 23 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC00603.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF86997.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002311; AAC00603.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC005292; AAF86997.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30359.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30360.1; -; Genomic_DNA.
DR PIR; F86366; F86366.
DR RefSeq; NP_001185071.1; NM_001198142.2. [F4I4P3-2]
DR RefSeq; NP_173737.1; NM_102172.2. [F4I4P3-1]
DR AlphaFoldDB; F4I4P3; -.
DR SMR; F4I4P3; -.
DR STRING; 3702.AT1G23230.1; -.
DR PaxDb; F4I4P3; -.
DR PRIDE; F4I4P3; -.
DR EnsemblPlants; AT1G23230.1; AT1G23230.1; AT1G23230. [F4I4P3-1]
DR EnsemblPlants; AT1G23230.2; AT1G23230.2; AT1G23230. [F4I4P3-2]
DR GeneID; 838932; -.
DR Gramene; AT1G23230.1; AT1G23230.1; AT1G23230. [F4I4P3-1]
DR Gramene; AT1G23230.2; AT1G23230.2; AT1G23230. [F4I4P3-2]
DR KEGG; ath:AT1G23230; -.
DR Araport; AT1G23230; -.
DR TAIR; locus:2028040; AT1G23230.
DR eggNOG; KOG1883; Eukaryota.
DR InParanoid; F4I4P3; -.
DR OMA; RGHQIQD; -.
DR OrthoDB; 31667at2759; -.
DR PRO; PR:F4I4P3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I4P3; baseline and differential.
DR Genevisible; F4I4P3; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0010628; P:positive regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR021629; Mediator_Med23.
DR PANTHER; PTHR12691; PTHR12691; 1.
DR Pfam; PF11573; Med23; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1615
FT /note="Mediator of RNA polymerase II transcription subunit
FT 23"
FT /id="PRO_0000418123"
FT REGION 40..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1230..1270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..64
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1230..1246
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1253..1270
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 477..495
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043991"
FT VAR_SEQ 754..758
FT /note="SQLAQ -> S (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043992"
SQ SEQUENCE 1615 AA; 180096 MW; 67EF3CD2CC11602C CRC64;
MDQSQRTVAA TPSSSRSYQF HPARAAIIDL FNLYLGRGSR QKPDESLRDP PNKSQKRVHA
PNRDLPPRNE QFLLDFELLQ SQFNDPEQLR TITESVLISL VVQCSNHAPR AEFLLFALRT
LCRISYINWD TFLPSLLSSV SAAEASLSQG VQAAAATAGS SATSSQSVVP VSVNPTSLLP
SAHGIGSPSA SEVKSVENGQ QIARAGQIVR ENAMRNSQRI RAAAVNSLRQ LSCKIILIGV
ESSLKPVTHA EIFQYMMNWL VNWDRRDLGT EDSVGKSWRS EKTLAEWLRS CLDVIWLLVE
EGESRIPFYE LLRSGLQFIE NIPDDEALFT LIMEIHRRRD AMAMHMLMLD QHLHCPSFGT
HRIVSQITAN VPPEAVPHLR HSPITYPSVL GEPLYGEDLA MSIPKGSLDW ERAVRCIRHA
IRTTPSPDWW KRVLVVAPCY RPSTQAGPIP GAVFTSDMIC EAIIDRIVEL LKLTNSGNDC
FGIDLVSVTF PPLYADANCW QEWLVFSDIF FFLIKSGCTD FVDFIDKLVL RLNGVDNHIL
RTNHVTWLLA QIIRVELVMT ALNSDAKKVE TTRKILSFHR EDRNSDPNNP QSVLLDFVSS
CQNLRIWSLS TTTRAYLNNE QLLKGKQIDE WWRSKGERMM DYMNMDDRSI GMFWVVSYTM
AQPACETVIN WLSSAGMAEL PGLQPNDRVM MTQEVTPLPM SLLSGFSMNL CLKLALQMEE
ALFVSQVVPS IAMVETYTRL LLISPHSMFR SHFSQLAQRN ASLLSKPGVT LLVLEILNYR
LLPLYRYQGK SKTLMYDVTK IISALKGKRG DHRIFRLAEN LCMNLILSLR DFFSVKREGK
GPTEFTETLN RITIMTLAIT IKTRGIADPD HMVYLQTMLE QILATSQHTW SEKTMRHFPS
LLRETLKGRV DKRGLSIQAW QQAETTVINQ CTQLLSPSAE PAYVSTYLSH SFPQHRQYLC
AGACLLMQGH AENINSTNLA RVLREVSPEE VTANIYTLVD VLLHHVHVDL QQGQSLEAVL
DKAGANLAFF FWTHEMLPLD IFLLALIDRD DDPHALIIAM SLLKTPDLLL RIKNYCQNRG
SPEHWLVTQV FKRNELQKAL GNHLSWKDRY PTFFDDIAAR LLPVIPLVLY RLIENNAMEQ
ADNLLLAHSH FLAYHPLRFT FVRDILAYFY GHLPGKLVLR MLKVLDLSKI PFSESFPQYI
SPTGAPVCPP LDYFASLLLN LVNNVIPPLS SSSNCSSRSG SMADILNSSA RPPHGKTPGT
SQPGPANASE GQKAFYQIQD PGTYTQLVLE TAVIEILSLP VSAAQIVSSL VQIIVNIQST
LIQSGNGFHG AANGVGQGSV LPTSPSGGST DSMSASRSTC LIPGINTASF VSRSGYTCQQ
LSCLLIQACG LLLAQLPPDF HVQLYLEAAR VTRETWWLKD GKRSQGELDS AVGYALMDPT
WAAQDNTSTA IGNIVALLHA FFSNLPQEWL DGTNAIITNL RPVTSVAMLR VVFRIMGPLL
PRLASTHTLF NKTLMLLLSA LVDVFGKTAQ TTAPVEASQI ADLIDFLHHI IHYEGQGGAV
QTSSKPRPDI LALIGRAAET LRPDVQHLLA HLKTNPNSSI YAAAHQQNTA KTNTS
