MED23_CAEEL
ID MED23_CAEEL Reviewed; 1587 AA.
AC Q10669; O45497; Q9U3G5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 23;
DE AltName: Full=Mediator complex subunit 23;
DE AltName: Full=Mediator complex subunit sur-2;
GN Name=sur-2; Synonyms=mdt-23; ORFNames=F39B2.4;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=Bristol N2; TISSUE=Embryo;
RX PubMed=7557379; DOI=10.1101/gad.9.18.2251;
RA Singh N., Han M.;
RT "sur-2, a novel gene, functions late in the let-60 ras-mediated signaling
RT pathway during Caenorhabditis elegans vulval induction.";
RL Genes Dev. 9:2251-2265(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION.
RX PubMed=12130541; DOI=10.1101/gad.998402;
RA Howard R.M., Sundaram M.V.;
RT "C. elegans EOR-1/PLZF and EOR-2 positively regulate Ras and Wnt signaling
RT and function redundantly with LIN-25 and the SUR-2 Mediator component.";
RL Genes Dev. 16:1815-1827(2002).
RN [4]
RP FUNCTION, AND MUTAGENESIS OF GLY-1347.
RX PubMed=15268855; DOI=10.1016/j.cub.2004.07.022;
RA Nicholas H.R., Hodgkin J.;
RT "The ERK MAP kinase cascade mediates tail swelling and a protective
RT response to rectal infection in C. elegans.";
RL Curr. Biol. 14:1256-1261(2004).
RN [5]
RP FUNCTION, AND INTERACTION WITH LET-19.
RX PubMed=15790964; DOI=10.1242/dev.01776;
RA Yoda A., Kouike H., Okano H., Sawa H.;
RT "Components of the transcriptional Mediator complex are required for
RT asymmetric cell division in C. elegans.";
RL Development 132:1885-1893(2005).
RN [6]
RP FUNCTION.
RX PubMed=15901674; DOI=10.1101/gad.333505;
RA Berset T.A., Hoier E.F., Hajnal A.;
RT "The C. elegans homolog of the mammalian tumor suppressor Dep-1/Scc1
RT inhibits EGFR signaling to regulate binary cell fate decisions.";
RL Genes Dev. 19:1328-1340(2005).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC regulated gene transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional pre-initiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Functions
CC downstream of receptor let-23 and let-60/Ras during vulval induction
CC likely by down-regulating the expression of phosphatase dep-1 and lin-
CC 12/Notch in vulva precursor cell descendants with a primary cell fate
CC (PubMed:7557379, PubMed:15901674). Acts to repress beta-catenin target
CC genes (PubMed:12130541). Required for asymmetric division of T-cells
CC (PubMed:15790964). Plays a role in responses to M.nematophilum-mediated
CC bacterial infection by promoting tail swelling and preventing
CC constipation (PubMed:15268855). {ECO:0000250|UniProtKB:Q15648,
CC ECO:0000269|PubMed:12130541, ECO:0000269|PubMed:15268855,
CC ECO:0000269|PubMed:15790964, ECO:0000269|PubMed:15901674,
CC ECO:0000269|PubMed:7557379}.
CC -!- SUBUNIT: Component of the Mediator complex (By similarity). Interacts
CC with let-19/mdt-13. {ECO:0000250, ECO:0000269|PubMed:15790964}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q10669-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q10669-2; Sequence=VSP_004435;
CC -!- DEVELOPMENTAL STAGE: Highest levels in embryos and larvae. Expressed in
CC vulval precursor cells at the time of vulval determination.
CC {ECO:0000269|PubMed:7557379}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 23 family.
CC {ECO:0000305}.
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DR EMBL; U33051; AAA85507.1; -; mRNA.
DR EMBL; Z92834; CAB07385.2; -; Genomic_DNA.
DR EMBL; Z92834; CAB07394.2; -; Genomic_DNA.
DR PIR; T21986; T21986.
DR PIR; T21993; T21993.
DR RefSeq; NP_001021463.1; NM_001026292.3. [Q10669-2]
DR RefSeq; NP_493575.2; NM_061174.5. [Q10669-1]
DR AlphaFoldDB; Q10669; -.
DR SMR; Q10669; -.
DR BioGRID; 38728; 13.
DR IntAct; Q10669; 1.
DR STRING; 6239.F39B2.4b; -.
DR EPD; Q10669; -.
DR PaxDb; Q10669; -.
DR PeptideAtlas; Q10669; -.
DR EnsemblMetazoa; F39B2.4a.1; F39B2.4a.1; WBGene00006349. [Q10669-1]
DR EnsemblMetazoa; F39B2.4b.1; F39B2.4b.1; WBGene00006349. [Q10669-2]
DR GeneID; 173345; -.
DR KEGG; cel:CELE_F39B2.4; -.
DR UCSC; F39B2.4b; c. elegans. [Q10669-1]
DR CTD; 173345; -.
DR WormBase; F39B2.4a; CE28023; WBGene00006349; sur-2. [Q10669-1]
DR WormBase; F39B2.4b; CE28024; WBGene00006349; sur-2. [Q10669-2]
DR eggNOG; KOG1883; Eukaryota.
DR GeneTree; ENSGT00390000010380; -.
DR HOGENOM; CLU_244777_0_0_1; -.
DR InParanoid; Q10669; -.
DR OMA; HALYVTC; -.
DR OrthoDB; 94121at2759; -.
DR PhylomeDB; Q10669; -.
DR SignaLink; Q10669; -.
DR PRO; PR:Q10669; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00006349; Expressed in embryo and 4 other tissues.
DR GO; GO:0016592; C:mediator complex; IDA:WormBase.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IBA:GO_Central.
DR GO; GO:0042176; P:regulation of protein catabolic process; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:WormBase.
DR InterPro; IPR021629; Mediator_Med23.
DR PANTHER; PTHR12691; PTHR12691; 1.
DR Pfam; PF11573; Med23; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Developmental protein; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..1587
FT /note="Mediator of RNA polymerase II transcription subunit
FT 23"
FT /id="PRO_0000072315"
FT REGION 1374..1484
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1387..1404
FT /note="Acidic"
FT REGION 1567..1587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1382..1406
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1407..1430
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1438..1484
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 505
FT /note="Y -> FLD (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_004435"
FT MUTAGEN 1347
FT /note="G->I: In e2706; lack of tail swelling and mild
FT constipation following M.nematophilium infection.
FT Hyperinduction of vulval epidermal cells."
FT /evidence="ECO:0000269|PubMed:15268855"
SQ SEQUENCE 1587 AA; 183907 MW; A125FCA74922B11C CRC64;
MTDKPGSAED TIEMDDTCSP QFVQEVNRIK SQIKSLVEEN HTRKFFRPLT SNIGDETAIL
RIQFNNMMSK MEEKEKQSLV KELIKMVHHV AEKNSPDRVF VGTNYERVVD QLLRYAHQKG
AISTNLCAEG LIMTSDFRLC SRICQEKWKF INDCIPKIDY KGIRNILRYI LESQLRRLPY
SLSPEQVNEI RIVENVILHI VDRDSNLMPP LITLSEIMRG MPKQALMLPR LTEKLASLSV
HFRPIADLSH VCGRGFVYPI PLHPSFYPLT SCWEEHGLNA SNTIVQPHHT LPYRPEHTST
YLYTLYMILR QPLGKDSLHP PSKTKTKTNW EMLISVMICE SMAEAESLPE TEPIPRYQWD
NIVNIVIYGI TQHLLVPKTF FNVLKNLIKR CKYTRARDEV MWIVFQVVGS LSNLTRLDDA
VTEIVELYNE LFDGDVVWMG ASDHPALFAR FLAAAGTWMI LEKDFADKMP PANETIKSHI
KFIQDGVDNF DSSNTAMLAV LSNIYRTDTK MGKLIVPTLQ QMLESIDDTK SLFELSYKRM
AVNCFSAFPV EFMEALTFRS KKTLLIQCFQ PLRSFSTVRL PSPAVFETVA KICESEDYEM
AVKDMEHLAQ RSLHVATAAD RSSGEHQNVQ AKDQCYFLFD FLVYRLPHLH AYSKYSSTVN
ALSYFTQHIP NNPQNHQIYR LMEQFLMRRW CWAGFHGCIT AHTQMFGTSY KDNTMMRHMA
YPKTFSVPDQ YPFAINPEIF KMAIYSFLRA VKITAQDIAI EKTMFPTIIN GFGWPEKSTS
YFPKWALDAI KASDTSNAVN TEEILSDVRN TARMHTSLTP NQFVIRYGED RDPATSHCML
AVLFHFAYNS VDSTYNITSE FYEVMEKKTP KEIVVMGNYL VDYIIADAKT QDCNEKTFKN
IAKAAALLVF QFQVLRADRL LLSLIMHPAT DEDALICIQI ANEFILTPEF QERIRWYHQN
VPKKDHFPTE YIKAIVKYHD AFPEFEACEL VRSYDSSSNV HMPTYYGCLI ERLLPIMDQY
LHVALEQQGY KLNPQILQSV SMLYKFHAMP IHFMYSVLFT SHGLMSGPDA KSFVLAFATQ
IEECHLTEAF EKFNHQKSSR EQLIMELIDR MSASLDFILT PPPFVAKDWK IAELSPGAQT
LYLACIELMA SPHSPETLVA AMINVMQMKP HARPFNIVNL TALLLTALPA AYSNALHDEF
VAVFVNGETA NLKFEEIVFD NYEESLLLNL PNRARTINVI SQQYWLHCSL SLLNFFSHEY
VPRILEHVKT EKDLWYTLRL VMPYLRRYYE NWDTAKQMRS QRENFGPLHI VKLVFQKLGS
MAEEGVEIVY EQHLCDLFYN CKYFFAGDFL RNTAITEFAK LPEKMRDRLK FYVSQSEPTA
EQETPPEKEK SPEKEKEQEQ EQHVKAHQPL ESTPSVSSLP QMQHHLQQAP LLPSHQMMPP
PQQHSSSLQH HLQHHTSTHQ MMDTSQHQTI QQQSNHPTQQ QLQHQIPNMS MHQQMGPQYP
GAVFHHPSGP VGHVPMQYGM GHHMQQHPHL PHHQQMPAPM HTMNPMMQNM TPQQQYLYMQ
QLQQHQQHQQ YMQQQQQHHH QHQQQPH
