ID   MED23_DANRE             Reviewed;        1376 AA.
AC   Q5RIW8; B8A4G5; Q68EG9;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 2.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 23;
DE   AltName: Full=Cofactor required for Sp1 transcriptional activation subunit 3;
DE            Short=CRSP complex subunit 3;
DE   AltName: Full=Mediator complex subunit 23;
GN   Name=med23; Synonyms=crsp3; ORFNames=si:ch211-272f15.2, zgc:91979;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q5RIW8-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q5RIW8-2; Sequence=VSP_028386;
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 23 family.
CC       {ECO:0000305}.
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DR   EMBL; BX072559; CAX13819.1; -; Genomic_DNA.
DR   EMBL; BC080264; AAH80264.1; -; mRNA.
DR   RefSeq; NP_001003990.1; NM_001003990.1. [Q5RIW8-2]
DR   AlphaFoldDB; Q5RIW8; -.
DR   SMR; Q5RIW8; -.
DR   STRING; 7955.ENSDARP00000116237; -.
DR   PaxDb; Q5RIW8; -.
DR   PRIDE; Q5RIW8; -.
DR   Ensembl; ENSDART00000135760; ENSDARP00000119918; ENSDARG00000029157. [Q5RIW8-1]
DR   GeneID; 445483; -.
DR   KEGG; dre:445483; -.
DR   CTD; 9439; -.
DR   ZFIN; ZDB-GENE-040724-82; med23.
DR   eggNOG; KOG1883; Eukaryota.
DR   GeneTree; ENSGT00390000010380; -.
DR   HOGENOM; CLU_002773_0_0_1; -.
DR   InParanoid; Q5RIW8; -.
DR   PhylomeDB; Q5RIW8; -.
DR   Reactome; R-DRE-212436; Generic Transcription Pathway.
DR   PRO; PR:Q5RIW8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 20.
DR   Bgee; ENSDARG00000029157; Expressed in gastrula and 22 other tissues.
DR   ExpressionAtlas; Q5RIW8; baseline.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0072359; P:circulatory system development; IMP:ZFIN.
DR   GO; GO:0050768; P:negative regulation of neurogenesis; IMP:ZFIN.
DR   GO; GO:0043473; P:pigmentation; IMP:ZFIN.
DR   GO; GO:0010628; P:positive regulation of gene expression; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR021629; Mediator_Med23.
DR   PANTHER; PTHR12691; PTHR12691; 1.
DR   Pfam; PF11573; Med23; 1.
PE   2: Evidence at transcript level;
KW   Activator; Alternative splicing; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..1376
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   23"
FT                   /id="PRO_0000305933"
FT   REGION          1346..1376
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..24
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.2"
FT                   /id="VSP_028386"
SQ   SEQUENCE   1376 AA;  156882 MW;  9F064EABE2FB3048 CRC64;
     METQLQSIFE DVVKTEVIEE AFAGMFMDSP EDEKTKLTSC LGAFRQFWST LPQESHEQCV
     QWIVRFIHGQ HSPKRIAFLY DCLAMAVETS LLPPRMVCQA LISSDSLEWE RTQLWALTFK
     LIRKIIGGVD YKGVRDLLKA VLDKIQTIPN FVSSAVVQQL LAAREVVEYI LDRNACLLPA
     YFAVTEIRKL CPEGALSHWL LGSLISDFVD SFRPTARINS ICGRCSLLPV VNNSGAICNS
     WKLDPTTLRF PLRGMLPYDK DLFDPQTGLL RYVLEQPYSR DMVCNMLGLN KQHKQRCPVL
     EDQLVDLVVY AMERSETEEQ FDDGGTSQLL WQHLSSQLIF FVLFQFASFP HMVLSLHQKL
     AGRGLIKGRD HLMWVLLQFI SGSIQKNALG DFLPVMKLFD LLYPEKECIP VPDINKPQST
     HAFAMTCIWI HLNRKAQNDN SKLQIPIPHS LKLHHEFLQQ SLRNKSLPMT DYKIALLCNA
     YSTNSECFTL PMGVLVETIY GNGSMRITLP GTNCMASGSV TPLPMNLLDS LTVHAKMSLI
     HSIATRVIKL AHAKSSLALA PALVETYSRL LVYMEIESLG IKGFISQLLP NVFKSHAWGI
     LHTLLEMFSY RMHHIQPHYR VQLLSHLHSL AAVPQTNQNQ LHLCVESTAL RLITALGSSE
     VQPQFTRFLN DPKTVLSAES EELNRALILT LARATHVTDF FTGSDSIQGT WCKDILQTIM
     NFTPHNWASH TLSCFPAPLQ AFFKQNNVPQ ESRFNLKKNV EEEYRKWKSM TNENDIITHF
     SMQGSPPLFL CLLWKMLLET DHINQIGFRV LERIGARALV AHVRTFADFL VYEFSTSAGG
     QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLLL LKPNDFRNRV
     SDFVKENAPE HWLQSDWHTK HMTYHKKYPE KLYFEGLAEQ VNPPIQLQSQ YLPIYFGNVC
     LRFLPVFDIV IHRFLELLPV SKSLETLLDH LGGLYKFHDR PVTYLYNTLH YYERHLRDRT
     NLKRKLVHAI MSSLKDNRTP GWCLSETYLK CGMNAREDNV WIPDDTYYCK LIGRLVDTMA
     GKSPGPFPNC DWRFNEFPNP AAHALHVTCV ELMALAVPGK DVGNALLNVV LKSQPLVPRE
     NITAWMNAIG LVITALPEPY WIVLHDRIVS VISSAVLSSG SDWLGYPFQL LDFTACHQSY
     SEMHCSYVLA LAHAVWHHSS IGQLSLIPKF LSEVLKPIVK TEFQLLYVYH LVGPFLQRFQ
     QERTRCMLEI GVAFYEMLQA VDQHSNHLAY MDPICDFLYH IKYMFTGDSV KDQVERIICS
     LRPAMRLRLR FITHISKMEP AAVPVSNSSS VQQTSSASSP TAQSTAGAAI PLSVTQ