MED23_DROME
ID MED23_DROME Reviewed; 1439 AA.
AC Q9W1X7; A2RVE3;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 23;
DE AltName: Full=Mediator complex subunit 23;
DE AltName: Full=dSUR2;
DE AltName: Full=dTRAP150;
GN Name=MED23; Synonyms=Trap150; ORFNames=CG3695;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, AND INTERACTION WITH HSF.
RX PubMed=15297616; DOI=10.1073/pnas.0401985101;
RA Kim T.W., Kwon Y.-J., Kim J.M., Song Y.-H., Kim S.N., Kim Y.-J.;
RT "MED16 and MED23 of Mediator are coactivators of lipopolysaccharide- and
RT heat-shock-induced transcriptional activators.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:12153-12158(2004).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC transcriptional activation in response to heat shock. {ECO:0000250,
CC ECO:0000269|PubMed:15297616}.
CC -!- SUBUNIT: Component of the Mediator complex (By similarity). Interacts
CC with Hsf. {ECO:0000250, ECO:0000269|PubMed:15297616}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 23 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABM92808.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE013599; AAF46925.1; -; Genomic_DNA.
DR EMBL; BT029934; ABM92808.1; ALT_INIT; mRNA.
DR RefSeq; NP_611735.1; NM_137891.3.
DR AlphaFoldDB; Q9W1X7; -.
DR SMR; Q9W1X7; -.
DR BioGRID; 63250; 34.
DR STRING; 7227.FBpp0289089; -.
DR PaxDb; Q9W1X7; -.
DR PRIDE; Q9W1X7; -.
DR EnsemblMetazoa; FBtr0071903; FBpp0071814; FBgn0034795.
DR GeneID; 37639; -.
DR KEGG; dme:Dmel_CG3695; -.
DR CTD; 9439; -.
DR FlyBase; FBgn0034795; MED23.
DR VEuPathDB; VectorBase:FBgn0034795; -.
DR eggNOG; KOG1883; Eukaryota.
DR GeneTree; ENSGT00390000010380; -.
DR HOGENOM; CLU_002773_0_0_1; -.
DR InParanoid; Q9W1X7; -.
DR OMA; HALYVTC; -.
DR OrthoDB; 94121at2759; -.
DR PhylomeDB; Q9W1X7; -.
DR BioGRID-ORCS; 37639; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37639; -.
DR PRO; PR:Q9W1X7; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034795; Expressed in wing disc and 18 other tissues.
DR Genevisible; Q9W1X7; DM.
DR GO; GO:0016592; C:mediator complex; ISS:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR InterPro; IPR021629; Mediator_Med23.
DR PANTHER; PTHR12691; PTHR12691; 1.
DR Pfam; PF11573; Med23; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1439
FT /note="Mediator of RNA polymerase II transcription subunit
FT 23"
FT /id="PRO_0000305937"
FT REGION 358..625
FT /note="Interaction with Hsf"
FT REGION 1338..1372
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1401..1439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 282..318
FT /evidence="ECO:0000255"
FT COMPBIAS 1401..1432
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 3
FT /note="T -> A (in Ref. 3; ABM92808)"
FT /evidence="ECO:0000305"
FT CONFLICT 504
FT /note="G -> S (in Ref. 3; ABM92808)"
FT /evidence="ECO:0000305"
FT CONFLICT 1319
FT /note="M -> L (in Ref. 3; ABM92808)"
FT /evidence="ECO:0000305"
FT CONFLICT 1366
FT /note="H -> HQ (in Ref. 3; ABM92808)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1439 AA; 167065 MW; 1E07DD5F62EE5A81 CRC64;
METQVIDTVN EFLKVDSLDE AFVSVIVFKP NTEQERATRF ANDLVTAFGN VAQENREQVL
RLYLLRAAGA SGYHIKVLMA ALVKLVDAHV ITARMLCDKV LMCEKLDFEH RTFWIESFRL
IKRVIVQVDY KGVREIMKVC RDKAQWFPLN VNVTYMPQLL AVEDILRFIF DRNNCLLPAY
FIANEIMRPF PYHWKLNRLM TDFVEEFRTT AQMVSIIGHA SMLPIVEHFG YADHMMNSWR
LDHNTLKFNF KGSLPYEPEL LEEQKPLLRY VLEQPYSREM VSQMLNLQKH QKQRYNALEE
QLVNLIVQAM EMTEANDATA GSGFNSSDEQ ITPYEWMWLH LSSQLIYFVL FQFVSFMHIV
LALHEKLSKL ELRKGRDQLM WILLQFISGS IQKNPITNFL PVFRLFDLLY PELEPLKLPD
INKSSMVRHM APICVWIHLM KKARVENMNI TRPLPIALKN HYDFLQHLVT ANTMMNMTLG
NDFRIILICN AYSTNQEYFG RPMGLLLDAL NGTSKSPNGG QIPAVTFSVT VLDSLTVHSK
MSLIHSFVTQ MLKQAQSKGQ VPAAALLETY ARLLVYTEIE SLGIKGFLSQ LMPTVFKNHA
WAMLHTLMEM FSYRLHHVPT HYRVQLLSLL HSLSSVPQTN KMQLNLCFES TALRLITSIG
SAEFQPQFSR YFNDKSPGAV ASNESEELNR VLILTLARSM HVHGGGDEMQ GWCKDFLSNI
IQHTPHSWPM HSLACFPPAL NEYFTQNNQP PENKQQLKKA VEEEYRTWTS MTNENDIIAH
FLRPTTNPLF LCLLFKIIWE TENISPVAYK ILEGISARAL STHLRKFCDY LVAEVASSSD
GRDFIHKCVD TINNMIWKFN VVTIDRVVLC LALRTHEGNE AQVCFLIIQL LLLKASELRN
RVQEFCKDNN PDHWKQSNWQ DKHLSFHQKY PEKFALDESA SQIPLPVYFS NVCLRFLPVL
DVVVHRFIEL TITNVHQILG FILDHLSILY KFHDRPITYL YNTLHYYERI LRDRPALKKK
LVGAITSAFS EIRPPNWSVS EPYKVYLQSQ DSLWTPELSY YMSLIRRLAD TISGKNVFYS
TDWRFNEFPN APTHALYVTC VELLGLPVAP PLVASNLIDV IVSGYAVIPQ KDIHSYINAV
GIVLAALPEP YWSGIYDRLQ DMLNTPNMLN WTYRFNAFEL FNFKTVREAM LEKTYAVVLA
VAHSVFHHMG AFKLAAMTRY LKEKLKPCVR TEQQLLYLCH VFGPFLQRIE LEKPNAVAGI
AVLLYEILEI VDKHHGPKPL QYMDQICDFL YHIKYIHVGN IIKNESEAII KRLRPLLQMR
LRFITHLNLE DIHTEKINDN TSNNAITSQT QSPMQTQHQQ QPQQPHQQQQ QQQQQQQQQQ
QQQQQQQQMQ QQQINAVQTT SVPLGSGGNL QQQQQINQQQ QMYMQHMQQH QHMQNMRHN
