ID   MED23_RAT               Reviewed;        1367 AA.
AC   Q5EB59;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 23;
DE   AltName: Full=Cofactor required for Sp1 transcriptional activation subunit 3;
DE            Short=CRSP complex subunit 3;
DE   AltName: Full=Mediator complex subunit 23;
GN   Name=Med23; Synonyms=Crsp3;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RX   PubMed=15057822; DOI=10.1038/nature02426;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 751-1367.
RC   TISSUE=Liver;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional pre-initiation complex with RNA polymerase II
CC       and the general transcription factors. Also required for
CC       transcriptional activation subsequent to the assembly of the pre-
CC       initiation complex. Required for transcriptional activation by
CC       adenovirus E1A protein. Required for ELK1-dependent transcriptional
CC       activation in response to activated Ras signaling (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC       MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC       MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC       MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC       CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC       module termed the CDK8 module. Mediator containing the CDK8 module is
CC       less active than Mediator lacking this module in supporting
CC       transcriptional activation. Individual preparations of the Mediator
CC       complex lacking one or more distinct subunits have been variously
CC       termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CDK8, CEBPB,
CC       CTNNB1, ELK1 and GLI3. Interacts with the adenovirus E1A protein (By
CC       similarity). {ECO:0000250|UniProtKB:Q80YQ2,
CC       ECO:0000250|UniProtKB:Q9ULK4}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 23 family.
CC       {ECO:0000305}.
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DR   EMBL; AABR03000314; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC090022; AAH90022.1; -; mRNA.
DR   AlphaFoldDB; Q5EB59; -.
DR   SMR; Q5EB59; -.
DR   STRING; 10116.ENSRNOP00000036610; -.
DR   iPTMnet; Q5EB59; -.
DR   PhosphoSitePlus; Q5EB59; -.
DR   jPOST; Q5EB59; -.
DR   PaxDb; Q5EB59; -.
DR   PRIDE; Q5EB59; -.
DR   RGD; 1307671; Med23.
DR   eggNOG; KOG1883; Eukaryota.
DR   HOGENOM; CLU_002773_0_0_1; -.
DR   InParanoid; Q5EB59; -.
DR   PRO; PR:Q5EB59; -.
DR   Proteomes; UP000002494; Unplaced.
DR   Genevisible; Q5EB59; RN.
DR   GO; GO:0070847; C:core mediator complex; ISO:RGD.
DR   GO; GO:0016592; C:mediator complex; ISO:RGD.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0005667; C:transcription regulator complex; ISO:RGD.
DR   GO; GO:0000151; C:ubiquitin ligase complex; IDA:RGD.
DR   GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR   GO; GO:2000409; P:positive regulation of T cell extravasation; ISO:RGD.
DR   GO; GO:0016567; P:protein ubiquitination; IDA:RGD.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:RGD.
DR   InterPro; IPR021629; Mediator_Med23.
DR   PANTHER; PTHR12691; PTHR12691; 1.
DR   Pfam; PF11573; Med23; 1.
PE   2: Evidence at transcript level;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1367
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   23"
FT                   /id="PRO_0000305932"
FT   REGION          1343..1367
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1348..1367
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1367 AA;  156232 MW;  5C067F7FD80F3132 CRC64;
     METQLQSIFE EVVKTEIIEE AFPGMFMDTP EDEKTKLISC LAAFRQFWNG LSQESHEQCV
     QWIVKFIHGQ HSPKRISFLY DCLAMAVETG LLPPRMVCES LINSDTLEWE RTQLWALTFK
     LVRKIIGGVD YKGVRDLLKV ILEKILTIPN TVSSAVVQQL LAAREVIAYI LERNACLLPA
     YFAVTEIRKL YPEGKLPHWL LGNLVSDFVD TFRPTARINS ICGRCSLLPV VNNSGAICNS
     WKLDPATLRF PLKGLLPYDK DLFEPQTALL RYVLEQPYSR DMVCNMLGLN KQHKQRCPVL
     EDQLVDLVVY AMERSETEEK FDDGGTSQLL WQHLSSQLIF FVLFQFASFP HMVLSLHQKL
     AGRGLIKGRD HLMWVLLQFI SGSIQKNALA DFLPVMKLFD LLYPEKECIP VPDINKPQST
     HAFAMTCIWI HLNRKAQNDN SKLQIPIPHS LKLHHEFLQQ SLRNKSLQMN DYKIALLCNA
     YSTNSECFTL PMGALVETIY GNGIMRVPLP GTSCLASASV TPLPMNLLDS LTVHAKMSLI
     HSIATRVIKL AHTKSSVALA PALVETYSRL LVYMEIESLG IKGFISQLLP TVFKSHAWGI
     LHTLLEMFSH RMHHIQPHYR VQLLSHLHTL AAVAQTNQNQ LHLCVESTAL RLITALGSSE
     VQPQFTRFLN DPKTVLSAES EELNRALILT LARATHVTDF FTGSDSIQGT WCKDILQTIM
     TFTPHNWASH TLSCFPAPLQ AFFKQNNVPQ ESRFNLKKNV EEEYRKWKSM TDENEIITQF
     SMQGFPPLFL CLLWKMLLET DHISQIGYKV LERIGARALV AHVRTFADFL VYEFSTSAGG
     QQLNKCIEIL NDMVWKYNIV TLDRLILCLA MRSHEGNEAQ VCYFIIQLLL LKPNDFRNRV
     SDFVKENSPE HWLQSDWHTK HMSYHKKYPE KLYFEGLAEQ VDPPVPIQSP YLPIYFGNVC
     LRFLPVFDIV IHRFLELLPV SKSLETLLDH LGGLYKFHDR PVTYLYNTLH YYEMCLRNRD
     HLKRKLVHAI IGSLKDNRPQ GWCLSDTYLK HAMNAREDNP WVPEDSYYCK LIGRLVDTMA
     GKSPGPFPNC DWRFNEFPNP AAHALHVTCV ELMALAVPGK DVGNALLNVV LKSQPLVPRE
     NITAWMNAIG LIITALPEPY WIVLHDRIVS VISSSSLTSE TEWVGYPFRL FDFTACHQSY
     SEMSCSYTLA LAHAVWHHSS IGQLSLIPKF LTEVLLPVVK TEFQLLYVYH LVGPFLQRFQ
     QERTRCMIEI GVAFYDMLLN VDQCSTHLNY MDPICDFLYH MKYMFTGDSV KEQVEKIICN
     LKPALKLRLR FITHISKMEP AVPPQALSSG SPAPQANQVP TALPVTQ