ID   MED24_DANRE             Reviewed;         989 AA.
AC   Q2PW47; Q6NUW0;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 2.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 24;
DE   AltName: Full=Mediator complex subunit 24;
DE   AltName: Full=Protein lessen;
DE   AltName: Full=Thyroid hormone receptor-associated protein 4 homolog;
DE   AltName: Full=Trap100 homolog;
GN   Name=med24; Synonyms=lsn, thrap4, trap100;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=16396911; DOI=10.1242/dev.02215;
RA   Pietsch J., Delalande J.-M., Jakaitis B., Stensby J.D., Dohle S.,
RA   Talbot W.S., Raible D.W., Shepherd I.T.;
RT   "Lessen encodes a zebrafish trap100 required for enteric nervous system
RT   development.";
RL   Development 133:395-406(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-989.
RC   TISSUE=Pharyngula;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION.
RX   PubMed=16582438; DOI=10.1534/genetics.105.055152;
RA   Duerr K., Holzschuh J., Filippi A., Ettl A.-K., Ryu S., Shepherd I.T.,
RA   Driever W.;
RT   "Differential roles of transcriptional mediator complex subunits
RT   Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal
RT   development.";
RL   Genetics 174:693-705(2006).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). Required for
CC       proliferation of enteric nervous system precursors. Required for the
CC       development of dopaminergic amacrine cells and rod photoreceptor cells
CC       in the retina. {ECO:0000250, ECO:0000269|PubMed:16396911,
CC       ECO:0000269|PubMed:16582438}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Maternally expressed. Ubiquitously expressed
CC       through the 10 somite stage. Expression subsequently decreases in the
CC       trunk and tail while remaining high in anterior parts of the embryo. By
CC       24 hours post-fertilization (hpf) expression is no longer detectable in
CC       the trunk and tail, but is maintained in anterior parts of the embryo.
CC       At 48 hpf expression extends along almost the entire length of the gut
CC       and is particularly high in the dorsal forebrain, ventral midbrain, and
CC       the dorsal posterior part of the midbrain. At 60 hpf, expression in the
CC       gut is restricted to intestinal epithelial cells. At 72 hpf, expression
CC       in the CNS is limited to cells in the diencephalon, the ventral
CC       midbrain and the dorsal part of the midbrain, while expression outside
CC       the CNS is restricted to the pharyngeal endoderm. Expression in the
CC       pharyngeal endoderm persists through 96 hpf.
CC       {ECO:0000269|PubMed:16396911}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 24 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DQ300265; ABC17786.1; -; mRNA.
DR   EMBL; BC068408; AAH68408.1; -; mRNA.
DR   RefSeq; NP_001034151.1; NM_001039062.1.
DR   RefSeq; XP_009304761.1; XM_009306486.2.
DR   AlphaFoldDB; Q2PW47; -.
DR   SMR; Q2PW47; -.
DR   STRING; 7955.ENSDARP00000097153; -.
DR   PaxDb; Q2PW47; -.
DR   Ensembl; ENSDART00000157919; ENSDARP00000132097; ENSDARG00000099431.
DR   GeneID; 323621; -.
DR   KEGG; dre:323621; -.
DR   CTD; 9862; -.
DR   ZFIN; ZDB-GENE-030131-2341; med24.
DR   eggNOG; ENOG502QPJD; Eukaryota.
DR   GeneTree; ENSGT00390000016438; -.
DR   HOGENOM; CLU_007484_0_0_1; -.
DR   InParanoid; Q2PW47; -.
DR   OMA; TWQDICL; -.
DR   OrthoDB; 739187at2759; -.
DR   PhylomeDB; Q2PW47; -.
DR   Reactome; R-DRE-212436; Generic Transcription Pathway.
DR   PRO; PR:Q2PW47; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 12.
DR   Bgee; ENSDARG00000099431; Expressed in mature ovarian follicle and 33 other tissues.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0048484; P:enteric nervous system development; IMP:ZFIN.
DR   GO; GO:0061453; P:interstitial cell of Cajal differentiation; IMP:ZFIN.
DR   GO; GO:0046549; P:retinal cone cell development; IMP:ZFIN.
DR   GO; GO:0048538; P:thymus development; IMP:ZFIN.
DR   InterPro; IPR021429; Mediator_Med24.
DR   PANTHER; PTHR12898; PTHR12898; 1.
DR   Pfam; PF11277; Med24_N; 1.
PE   2: Evidence at transcript level;
KW   Activator; Developmental protein; Differentiation; Neurogenesis; Nucleus;
KW   Reference proteome; Repeat; Transcription; Transcription regulation.
FT   CHAIN           1..989
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   24"
FT                   /id="PRO_0000305915"
FT   MOTIF           343..347
FT                   /note="LXXLL motif 1"
FT   MOTIF           359..363
FT                   /note="LXXLL motif 2"
FT   MOTIF           447..451
FT                   /note="LXXLL motif 3"
FT   MOTIF           556..560
FT                   /note="LXXLL motif 4"
FT   MOTIF           787..791
FT                   /note="LXXLL motif 5"
FT   MOTIF           857..861
FT                   /note="LXXLL motif 6"
FT   CONFLICT        281
FT                   /note="E -> G (in Ref. 1; ABC17786)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   989 AA;  110862 MW;  D0B82E9018580A42 CRC64;
     MKVVNLKQAI LQAWKERWSD YQWAINIKKN CPKGATWDYL NLAEALLEQA MIGPSPNPLI
     LSYLKYAISS QMVSYSSVLT AISKFDDFSR ELCVKSLLEI MDMFSNRLSC HGKAEECIGL
     CRAMLCTVVW LLQGCAWYCE RLRDPNDFSA LESSLRACLE RMANLLHSTK NRALVHIARL
     EEQASWTNVE QAVLKLTENL SAVTNPTLKE SLEECVSLVK SIPQMLCLQC DPPVHTTFPS
     VHAFIMLEGT MNLTGETQPL VEQLMMIKRM QRIPAPLFVL EIWKACFTGL IESPEGNEEL
     KWTAFTFLKI PQVLLRLKKY PQGEKTQDFT EDVNVAFEYL LNLTPLLDKA DQRCNCDCLS
     LLLQECNKLG LLSEANTATL TAKRSEDREH APRLKTAENA NIQPNPGLIL RAEPTVTNIL
     KTVDADHSKS PEGLLGVLGH MLSGKSLDLL LAAAAATGKL KSFARKFIKL NEFPKHISGE
     GSKSASVRAL LFDISFLMLC HVVQTYGSEV ILSDPSPSSE TPFFETWLQT CMPEEGKTLN
     PDHPCFRPES GKVESLVALL NSSSEMKLVQ MKWHEICLST PAAILEVLNA WENGVLSVEA
     VQKITDNIKG KVCSMAICAV AWLVAHVRML GLDEREKPQT MIRQLMTPMS GENTLQFYHE
     RVVIMSSILE HMCADVFQQT GLSLRPAVEG QEPIPYRNLL PARLPIRQAL QSQFRKVLEK
     GWVDSHAFHL FENLLHMGGV FWFTNNLVKE LLKETRKEWA SRVVELLYSI FCLDTQQITL
     TLLGHILPRL LTDPAHWHSL ADPPGRALAK LSVWCALSSY SSHHKGQASA RQRKRHREDI
     EDYTSLFPLD DTQPSKLMRL LSSNEDENSM LSSPGDRSIS SSLSASQLHT VNMRDPLNRV
     LANLFLLISS QLGSKTAGPH TQFVQSFVEE CVDCSEQGSR SSILQFMPFT MISELVKLQS
     LAKPKTVLSI TDLSLPLGRR MAAKAIAAL