MED24_DROME
ID MED24_DROME Reviewed; 993 AA.
AC Q9VSF2; Q8MQZ3; Q9GYW5;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 24;
DE AltName: Full=Mediator complex subunit 24;
DE AltName: Full=dMED24;
DE AltName: Full=dTRAP100;
GN Name=MED24; Synonyms=Trap100; ORFNames=CG7999;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Southworth J.W., Kennison J.A.;
RT "Transcriptional coactivators in Drosophila.";
RL Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND INTERACTION WITH MED6 AND MED17.
RX PubMed=12021283; DOI=10.1074/jbc.m204144200;
RA Gu J.-Y., Park J.M., Song E.J., Mizuguchi G., Yoon J.H., Kim-Ha J.,
RA Lee K.-J., Kim Y.-J.;
RT "Novel Mediator proteins of the small Mediator complex in Drosophila SL2
RT cells.";
RL J. Biol. Chem. 277:27154-27161(2002).
RN [7]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-827 AND SER-829, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. Required for activated
CC transcription of the MtnA, MtnB and MtnD genes.
CC {ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex, which includes at least
CC CDK8, MED4, MED6, MED11, MED14, MED17, MED18, MED20, MED21, MED22,
CC MED27, MED28, MED30 and MED31. {ECO:0000269|PubMed:12021283}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 24 family.
CC {ECO:0000305}.
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DR EMBL; AF289998; AAG02463.1; -; mRNA.
DR EMBL; AE014296; AAF50469.2; -; Genomic_DNA.
DR EMBL; AY122223; AAM52735.1; -; mRNA.
DR EMBL; BT025232; ABF17923.1; -; mRNA.
DR RefSeq; NP_648185.1; NM_139928.5.
DR AlphaFoldDB; Q9VSF2; -.
DR SMR; Q9VSF2; -.
DR BioGRID; 64334; 5.
DR IntAct; Q9VSF2; 4.
DR STRING; 7227.FBpp0076417; -.
DR iPTMnet; Q9VSF2; -.
DR PaxDb; Q9VSF2; -.
DR PRIDE; Q9VSF2; -.
DR DNASU; 38914; -.
DR EnsemblMetazoa; FBtr0076694; FBpp0076417; FBgn0035851.
DR GeneID; 38914; -.
DR KEGG; dme:Dmel_CG7999; -.
DR CTD; 9862; -.
DR FlyBase; FBgn0035851; MED24.
DR VEuPathDB; VectorBase:FBgn0035851; -.
DR eggNOG; ENOG502QPJD; Eukaryota.
DR GeneTree; ENSGT00390000016438; -.
DR HOGENOM; CLU_007484_0_0_1; -.
DR InParanoid; Q9VSF2; -.
DR OMA; TWQDICL; -.
DR OrthoDB; 739187at2759; -.
DR PhylomeDB; Q9VSF2; -.
DR SignaLink; Q9VSF2; -.
DR BioGRID-ORCS; 38914; 0 hits in 1 CRISPR screen.
DR ChiTaRS; MED24; fly.
DR GenomeRNAi; 38914; -.
DR PRO; PR:Q9VSF2; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035851; Expressed in oviduct (Drosophila) and 26 other tissues.
DR Genevisible; Q9VSF2; DM.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:FlyBase.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB.
DR GO; GO:0035072; P:ecdysone-mediated induction of salivary gland cell autophagic cell death; IMP:FlyBase.
DR GO; GO:0043068; P:positive regulation of programmed cell death; IMP:FlyBase.
DR GO; GO:0035209; P:pupal development; IMP:FlyBase.
DR GO; GO:0043067; P:regulation of programmed cell death; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0035070; P:salivary gland histolysis; IMP:FlyBase.
DR InterPro; IPR021429; Mediator_Med24.
DR PANTHER; PTHR12898; PTHR12898; 1.
DR Pfam; PF11277; Med24_N; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..993
FT /note="Mediator of RNA polymerase II transcription subunit
FT 24"
FT /id="PRO_0000305919"
FT MOD_RES 827
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 829
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 684
FT /note="P -> T (in Ref. 4; AAM52735)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 993 AA; 111743 MW; 63146DB943200E7A CRC64;
MKENNKILQL IYVAWRERWT DSQWGINIKK VLPRGVSGDV YNLADCLMQQ ALIGSTANPL
VLNYLKHSLC AHLVSHAAVI RCIAKYDKLE RVYCITALLE FLASIVDGVT CRIKSEEAVL
PSSVVHLVYW LLQIFARTVQ HYELYGEISA EQSYMLDQTC VVIDRLSQQQ FLLSMLYVGC
HEELEICGRI RDKYATIKGS LTNSNFTLNA PQVEQQLQQL AFIEAKHLEM QPLNPPSTLE
KISCCVQPLL AVEVLLNPCK DTSYYVAELQ MLQRLKKYSN TRLFYEIIRA GFLTLSNVAD
TSPDTMWGAF MFFKMPHIIK QLHALQRIPG EQPPPADYIP ELVEALELLI EDNLLLDFMD
TKCSCNMIEF LLNDWTKQQL VNDVHVKKFA SQREAASQLL KKCDNGQQTP SNINFIKRAE
VPLSGVLKTL CTNKVQDMVN VLCQVPVGNS FELILSVATV EGRLKTFVSR LIQCNENSKP
VPGDLGKLCV IRSTLFDVSF LMLTSIVQTY GSDVVLSERG DSFFEKWVRE CMVERNKLKN
PRQILALCDD SIVDELLLSF SKPEASQLKP NNLSWQETCL NLPGVLHHVL IAWEQETLSS
ADVKSILDNI KRRLFSFSVC ATSFLCAYMY SVKETELLKP LNMIQQFLAP LTSEELSSQE
NAKERLALSY QIIRKMQHDV HPAPSTKSRL ISHSPLVEQF REVWRTVVDA GHLPVRAAQS
LESLLLAGGA AWLSTQLVEQ LLACKYTRDM SRTMDVVFAV MHLDIEKTTE ALLQYVVAPL
ILRRQGEDIN EPQSLVLARL CVYCIISCLE SRKGNGTSAL TAMKKRSRSH DDEELAANAA
KVRKVIGDGS DNSSDFTDTT TGAGLAALLG STSTSELRTT PLTLREPLQT SVQHIFAVFL
QFVSGDELSP KAVFVYQFIS LLVECGGERV APVLRLLPNG LVQQLLKVLV TDDIKVGLIS
RLYDLRIQAG RLSAVSDLCL WRNMQMARHS IHL
