MED24_RAT
ID MED24_RAT Reviewed; 987 AA.
AC Q4V8B3;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 24;
DE AltName: Full=Mediator complex subunit 24;
DE AltName: Full=Thyroid hormone receptor-associated protein 4;
GN Name=Med24; Synonyms=Thrap4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-860 AND SER-871, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with AR (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 24 family.
CC {ECO:0000305}.
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DR EMBL; BC097461; AAH97461.1; -; mRNA.
DR RefSeq; NP_001029251.1; NM_001034079.1.
DR AlphaFoldDB; Q4V8B3; -.
DR SMR; Q4V8B3; -.
DR STRING; 10116.ENSRNOP00000011947; -.
DR iPTMnet; Q4V8B3; -.
DR PhosphoSitePlus; Q4V8B3; -.
DR jPOST; Q4V8B3; -.
DR PaxDb; Q4V8B3; -.
DR PRIDE; Q4V8B3; -.
DR GeneID; 619436; -.
DR KEGG; rno:619436; -.
DR UCSC; RGD:1564565; rat.
DR CTD; 9862; -.
DR RGD; 1564565; Med24.
DR VEuPathDB; HostDB:ENSRNOG00000008711; -.
DR eggNOG; ENOG502QPJD; Eukaryota.
DR HOGENOM; CLU_007484_0_0_1; -.
DR InParanoid; Q4V8B3; -.
DR OMA; TWQDICL; -.
DR OrthoDB; 739187at2759; -.
DR PRO; PR:Q4V8B3; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000008711; Expressed in skeletal muscle tissue and 20 other tissues.
DR Genevisible; Q4V8B3; RN.
DR GO; GO:0070847; C:core mediator complex; ISO:RGD.
DR GO; GO:0016592; C:mediator complex; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0000151; C:ubiquitin ligase complex; IDA:RGD.
DR GO; GO:0004402; F:histone acetyltransferase activity; IDA:RGD.
DR GO; GO:0046966; F:nuclear thyroid hormone receptor binding; ISO:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:RGD.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:RGD.
DR GO; GO:0003712; F:transcription coregulator activity; ISO:RGD.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0016567; P:protein ubiquitination; IDA:RGD.
DR GO; GO:0019827; P:stem cell population maintenance; ISO:RGD.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISO:RGD.
DR InterPro; IPR021429; Mediator_Med24.
DR PANTHER; PTHR12898; PTHR12898; 1.
DR Pfam; PF11277; Med24_N; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW Transcription; Transcription regulation.
FT CHAIN 1..987
FT /note="Mediator of RNA polymerase II transcription subunit
FT 24"
FT /id="PRO_0000305913"
FT MOTIF 128..132
FT /note="LXXLL motif 1"
FT MOTIF 344..348
FT /note="LXXLL motif 2"
FT MOTIF 446..450
FT /note="LXXLL motif 3"
FT MOTIF 555..559
FT /note="LXXLL motif 4"
FT MOTIF 786..790
FT /note="LXXLL motif 5"
FT MOTIF 855..859
FT /note="LXXLL motif 6"
FT MOD_RES 860
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 871
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 987 AA; 110081 MW; 0456BD64D5245E56 CRC64;
MKVVNLKQAI LQAWKERWSD YQWAINMKKF FPKGATWDIL NLAEALLEQA MIGPSPNPLI
LSYLKYAISS QMVSYSSVLT AISKFDDFSR DLCVQALLDI MDMFCDRLSC HGKAEECIGL
CRALLSALHW LLRCTAASAE RLQEGLEAGS AVTGEKQLAL CLQCLEKTLS STKNRALLHI
AKLEEASSWT AIEHCLLKLG EILANLSNPQ LRSQAEHCGT LIRSIPTMLS VHSEQLHKTG
FPTIHALILL EGTMNLTGEM QPLVEQLMMV KRMQHIPTPL FVLEIWKACF VGLIESPEGT
QELKWTAFTY LKIPQVLVKL KKYFHGDKDF TEDVNCAFEF LLKLTPLLDK ADQRCNCDCT
NFLLQECNKQ GLLSEASFAS LVSKRTADRD PQLKSSENAN IQPNPGLILR AEPTVTNILK
TMDADHSKSP EGLLGVLGHM LSGKSLDLLL AAAAATGKLK SFARKFINLN EFTTHGSGES
TKTASVRALL FDISFLMLCH VAQTYGSEVI LSESSSGEEV PFFETWMQTC MPEEGKILNP
DHPCFRPDST KVESLVALLN NSSEMKLVQM KWHEACLSIS AAILEILNAW ENGVLAFESI
QKITDNIKGK VCSLAVCAVA WLVAHVRMLG LDEREKSLQM IRQLAGPLYS ENTLQFYNER
VVIMNSILEH MCADVLQQTA TQIKFPSTGV DTMPYWNLLP PKRPIKEVLT DIFAKVLEKG
WVDSRSIHIL DTLLHMGGVY WFCNNLIKEL LKETRKEHTL RAVQLLYSIF CLDMQQVTLV
LLGHILPGLL TDSSKWHSLM DPPGTALAKL AVWCALSSYS SHKGQASSRQ KKRHREDIED
YISLFPVEDM QPSKLMRLLS SNEDDASILS SPTDRSMNSS LSASQLHTVN MRDPLNRVLA
NLFLLISSVL GSRTAGPHTQ FVQWFMEECV DCLEQDSRGS ILQFMPFTTV SELVKVSAMS
SPKVVLAITD LSLPLGRQVA AKAIAAL
