MED25_ARATH
ID MED25_ARATH Reviewed; 836 AA.
AC Q7XYY2; F4ICJ2; Q9C6M3;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 25;
DE Short=AtMED25;
DE AltName: Full=Phytochrome and flowering time 1 protein;
DE AltName: Full=Phytochrome and flowering time regulatory protein 1;
GN Name=MED25; Synonyms=MED25_1, PFT1; OrderedLocusNames=At1g25540;
GN ORFNames=F2J7.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE, AND
RP SUBCELLULAR LOCATION.
RX PubMed=12815435; DOI=10.1038/nature01636;
RA Cerdan P.D., Chory J.;
RT "Regulation of flowering time by light quality.";
RL Nature 423:881-885(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP INDUCTION BY METHYL JASMONATE AND FUNGAL PATHOGEN.
RX PubMed=16183832; DOI=10.1104/pp.105.068544;
RA McGrath K.C., Dombrecht B., Manners J.M., Schenk P.M., Edgar C.I.,
RA Maclean D.J., Scheible W.R., Udvardi M.K., Kazan K.;
RT "Repressor- and activator-type ethylene response factors functioning in
RT jasmonate signaling and disease resistance identified via a genome-wide
RT screen of Arabidopsis transcription factor gene expression.";
RL Plant Physiol. 139:949-959(2005).
RN [5]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE
RP MEDIATOR COMPLEX, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [6]
RP FUNCTION.
RX PubMed=18790998; DOI=10.1104/pp.108.125468;
RA Wollenberg A.C., Strasser B., Cerdan P.D., Amasino R.M.;
RT "Acceleration of flowering during shade avoidance in Arabidopsis alters the
RT balance between FLOWERING LOCUS C-mediated repression and photoperiodic
RT induction of flowering.";
RL Plant Physiol. 148:1681-1694(2008).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19671879; DOI=10.1105/tpc.109.066910;
RA Kidd B.N., Edgar C.I., Kumar K.K., Aitken E.A., Schenk P.M., Manners J.M.,
RA Kazan K.;
RT "The mediator complex subunit PFT1 is a key regulator of jasmonate-
RT dependent defense in Arabidopsis.";
RL Plant Cell 21:2237-2252(2009).
RN [8]
RP INTERACTION WITH BBX20; PHL1; RAP2-2; ERF1B; ERF091; ERF095; ERF098 AND
RP ERF109.
RX PubMed=21343311; DOI=10.1093/mp/ssr002;
RA Ou B., Yin K.Q., Liu S.N., Yang Y., Gu T., Wing Hui J.M., Zhang L.,
RA Miao J., Kondou Y., Matsui M., Gu H.Y., Qu L.J.;
RT "A high-throughput screening system for Arabidopsis transcription factors
RT and its application to Med25-dependent transcriptional regulation.";
RL Mol. Plant 4:546-555(2011).
RN [9]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [10]
RP FUNCTION, INTERACTION WITH HB29; PHL1 AND DREB2A, AND DISRUPTION PHENOTYPE.
RX PubMed=21536906; DOI=10.1073/pnas.1002981108;
RA Elfving N., Davoine C., Benlloch R., Blomberg J., Braennstroem K.,
RA Mueller D., Nilsson A., Ulfstedt M., Ronne H., Wingsle G., Nilsson O.,
RA Bjoerklund S.;
RT "The Arabidopsis thaliana Med25 mediator subunit integrates environmental
RT cues to control plant development.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:8245-8250(2011).
RN [11]
RP FUNCTION.
RX PubMed=21985558; DOI=10.1111/j.1365-313x.2011.04815.x;
RA Inigo S., Alvarez M.J., Strasser B., Califano A., Cerdan P.D.;
RT "PFT1, the MED25 subunit of the plant Mediator complex, promotes flowering
RT through CONSTANS dependent and independent mechanisms in Arabidopsis.";
RL Plant J. 69:601-612(2012).
RN [12]
RP INTERACTION WITH DREB2A.
RX PubMed=22447446; DOI=10.1093/nar/gks265;
RA Blomberg J., Aguilar X., Braennstroem K., Rautio L., Olofsson A.,
RA Wittung-Stafshede P., Bjoerklund S.;
RT "Interactions between DNA, transcriptional regulator Dreb2a and the Med25
RT mediator subunit from Arabidopsis thaliana involve conformational
RT changes.";
RL Nucleic Acids Res. 40:5938-5950(2012).
RN [13]
RP INTERACTION WITH ABI5 AND MYC2.
RX PubMed=22822206; DOI=10.1105/tpc.112.098277;
RA Chen R., Jiang H., Li L., Zhai Q., Qi L., Zhou W., Liu X., Li H., Zheng W.,
RA Sun J., Li C.;
RT "The Arabidopsis mediator subunit MED25 differentially regulates jasmonate
RT and abscisic acid signaling through interacting with the MYC2 and ABI5
RT transcription factors.";
RL Plant Cell 24:2898-2916(2012).
RN [14]
RP INTERACTION WITH MBR1 AND MBR2.
RX PubMed=22992513; DOI=10.1104/pp.112.205500;
RA Inigo S., Giraldez A.N., Chory J., Cerdan P.D.;
RT "Proteasome-mediated turnover of Arabidopsis MED25 is coupled to the
RT activation of FLOWERING LOCUS T transcription.";
RL Plant Physiol. 160:1662-1673(2012).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. Positive regulator of shade
CC avoidance and of jasmonate signaling. Acts in repression of PhyB-
CC mediated light signaling and regulates the expression of FLOWERING
CC LOCUS T (FT) and of CONSTANS (CO). {ECO:0000269|PubMed:12815435,
CC ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:18790998,
CC ECO:0000269|PubMed:19671879, ECO:0000269|PubMed:21536906,
CC ECO:0000269|PubMed:21985558}.
CC -!- SUBUNIT: Component of the Mediator complex. Interacts with the
CC transcription factors BBX20, RAP2-2, ERF1B, ERF091, ERF095, ERF098,
CC ERF109, HB29, PHL1, DREB2A, ABI5 and MYC2. Interacts with the E3
CC ubiquitin-protein ligases MBR1 and MBR2. {ECO:0000269|PubMed:17560376,
CC ECO:0000269|PubMed:21343311, ECO:0000269|PubMed:21536906,
CC ECO:0000269|PubMed:22447446, ECO:0000269|PubMed:22822206,
CC ECO:0000269|PubMed:22992513}.
CC -!- INTERACTION:
CC Q7XYY2; P06492: UL48; Xeno; NbExp=2; IntAct=EBI-1386292, EBI-7489933;
CC Q7XYY2-1; O82132: DREB2A; NbExp=3; IntAct=EBI-15924435, EBI-1786840;
CC Q7XYY2-1; Q9FIP9: MYC3; NbExp=2; IntAct=EBI-15924435, EBI-15845995;
CC Q7XYY2-1; Q8GUN5-2: PHL1; NbExp=2; IntAct=EBI-15924435, EBI-15924466;
CC Q7XYY2-1; Q9SEZ1: ZHD11; NbExp=3; IntAct=EBI-15924435, EBI-1806317;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12815435}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7XYY2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7XYY2-2; Sequence=VSP_043993;
CC -!- INDUCTION: Down-regulated by methyl jasmonate and by infection with an
CC incompatible isolate of a necrotrophic fungal pathogen.
CC {ECO:0000269|PubMed:16183832}.
CC -!- DISRUPTION PHENOTYPE: Late-flowering phenotype when grown under long-
CC day conditions. Increased drought tolerance and sensitivity to
CC necrotrophic pathogens. Increased resistance to hemibiotrophic fungal
CC pathogen F.oxysporum. {ECO:0000269|PubMed:12815435,
CC ECO:0000269|PubMed:19671879, ECO:0000269|PubMed:21536906}.
CC -!- MISCELLANEOUS: The affinity between DREB2A and MED25 is reduced when
CC DREB2A is pre-bound to DNA.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 25 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50810.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY170377; AAO39425.1; -; mRNA.
DR EMBL; AC079281; AAG50810.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30639.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30640.1; -; Genomic_DNA.
DR PIR; G86385; G86385.
DR RefSeq; NP_001077596.1; NM_001084127.1. [Q7XYY2-2]
DR RefSeq; NP_173925.3; NM_102365.5. [Q7XYY2-1]
DR AlphaFoldDB; Q7XYY2; -.
DR SMR; Q7XYY2; -.
DR BioGRID; 24378; 27.
DR DIP; DIP-39923N; -.
DR IntAct; Q7XYY2; 6.
DR MINT; Q7XYY2; -.
DR STRING; 3702.AT1G25540.1; -.
DR iPTMnet; Q7XYY2; -.
DR PaxDb; Q7XYY2; -.
DR PRIDE; Q7XYY2; -.
DR ProteomicsDB; 250843; -. [Q7XYY2-1]
DR EnsemblPlants; AT1G25540.1; AT1G25540.1; AT1G25540. [Q7XYY2-1]
DR EnsemblPlants; AT1G25540.2; AT1G25540.2; AT1G25540. [Q7XYY2-2]
DR GeneID; 839141; -.
DR Gramene; AT1G25540.1; AT1G25540.1; AT1G25540. [Q7XYY2-1]
DR Gramene; AT1G25540.2; AT1G25540.2; AT1G25540. [Q7XYY2-2]
DR KEGG; ath:AT1G25540; -.
DR Araport; AT1G25540; -.
DR TAIR; locus:2031200; AT1G25540.
DR eggNOG; ENOG502QR8W; Eukaryota.
DR HOGENOM; CLU_010796_0_0_1; -.
DR InParanoid; Q7XYY2; -.
DR OMA; TPGMTQQ; -.
DR OrthoDB; 291305at2759; -.
DR PhylomeDB; Q7XYY2; -.
DR PRO; PR:Q7XYY2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q7XYY2; baseline and differential.
DR Genevisible; Q7XYY2; AT.
DR GO; GO:0016592; C:mediator complex; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; NAS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0031349; P:positive regulation of defense response; IMP:TAIR.
DR GO; GO:0009911; P:positive regulation of flower development; IMP:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0009585; P:red, far-red light phototransduction; IMP:TAIR.
DR GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR GO; GO:0010218; P:response to far red light; IMP:TAIR.
DR GO; GO:0010114; P:response to red light; IMP:TAIR.
DR GO; GO:0010091; P:trichome branching; IMP:TAIR.
DR GO; GO:1905499; P:trichome papilla formation; IMP:TAIR.
DR InterPro; IPR045105; Med25/PTOV1.
DR InterPro; IPR021419; Mediator_Med25_VWA.
DR PANTHER; PTHR12433; PTHR12433; 1.
DR Pfam; PF11265; Med25_VWA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Flowering;
KW Jasmonic acid signaling pathway; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..836
FT /note="Mediator of RNA polymerase II transcription subunit
FT 25"
FT /id="PRO_0000418124"
FT REGION 260..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 435..466
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 518..547
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 731..836
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 680..761
FT /evidence="ECO:0000255"
FT COMPBIAS 266..285
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 731..825
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..109
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043993"
SQ SEQUENCE 836 AA; 89566 MW; 9A9C81527BE4F384 CRC64;
MSSEVKQLIV VAEGTAALGP YWQTIVSDYL EKIIRSFCGS ELNGERNPVS TVELSLVIFN
SHGSYCACLV QRSGWTRDVD IFLHWLSSIQ FGGGGFNEVA TAEGLAEALM MFSPPSGQAQ
PSNDLKRHCI LITASNPHIL PTPVYRPRLQ NVERNENGDA QAESRLSDAE TVASYFAKCS
VSLSVVCPKQ LPTIRALYNA GKPNQQSADL SIDTAKNTFY LVLISENFVE ACAALSHSAT
NLPQTQSPVK VDRATVAPSI PVTGQPPAPV SSANGPIQNR QPVSVGPVPT ATVKVEPSTV
TSMAPVPSFP HIPAVARPAT QAIPSIQTSS ASPVSQDMVS NAENAPDIKP VVVSGMTPPL
RTGPPGGANV NLLNNLSQVR QVMSSAALAG AASSVGQSAV AMHMSNMIST GMATSLPPSQ
TVFSTGQQGI TSMAGSGALM GSAQTGQSPG PNNAFSPQTT SNVASNLGVS QPMQGMNQGS
HSGAMMQGGI SMNQNMMSGL GQGNVSSGTG GMMPTPGVGQ QAQSGIQQLG GSNSSAPNMQ
LSQPSSGAMQ TSQSKYVKVW EGNLSGQRQG QPVLITRLEG YRSASASDSL AANWPPTMQI
VRLISQDHMN NKQYVGKADF LVFRAMSQHG FLGQLQDKKL CAVIQLPSQT LLLSVSDKAC
RLIGMLFPGD MVVFKPQIPN QQQQQQQQLH QQQQQQQQIQ QQQQQQQHLQ QQQMPQLQQQ
QQQHQQQQQQ QHQLSQLQHH QQQQQQQQQQ QQQHQLTQLQ HHHQQQQQAS PLNQMQQQTS
PLNQMQQQTS PLNQMQQQQQ PQQMVMGGQA FAQAPGRSQQ GGGGGQPNMP GAGFMG
