MED25_BOVIN
ID MED25_BOVIN Reviewed; 746 AA.
AC A2VE44;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 25;
DE AltName: Full=Mediator complex subunit 25;
GN Name=MED25;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Thymus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. Required for RARA/RXRA-mediated
CC transcription (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CREBBP.
CC Interacts with ESR1, GR, RARA, RXRA and THRB in a ligand-dependent
CC fashion. Binds the Herpes simplex virus activator VP16 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 25 family.
CC {ECO:0000305}.
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DR EMBL; BC133565; AAI33566.1; -; mRNA.
DR RefSeq; NP_001075914.1; NM_001082445.1.
DR AlphaFoldDB; A2VE44; -.
DR BMRB; A2VE44; -.
DR SMR; A2VE44; -.
DR STRING; 9913.ENSBTAP00000040773; -.
DR PRIDE; A2VE44; -.
DR Ensembl; ENSBTAT00000043183; ENSBTAP00000040773; ENSBTAG00000008518.
DR GeneID; 533865; -.
DR KEGG; bta:533865; -.
DR CTD; 81857; -.
DR VEuPathDB; HostDB:ENSBTAG00000008518; -.
DR VGNC; VGNC:31362; MED25.
DR eggNOG; ENOG502QRN5; Eukaryota.
DR GeneTree; ENSGT00940000160439; -.
DR InParanoid; A2VE44; -.
DR OMA; NDQQKIP; -.
DR OrthoDB; 340324at2759; -.
DR Proteomes; UP000009136; Chromosome 18.
DR Bgee; ENSBTAG00000008518; Expressed in laryngeal cartilage and 104 other tissues.
DR ExpressionAtlas; A2VE44; baseline.
DR GO; GO:0070847; C:core mediator complex; IEA:Ensembl.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0046965; F:nuclear retinoid X receptor binding; IEA:Ensembl.
DR GO; GO:0001223; F:transcription coactivator binding; IEA:Ensembl.
DR GO; GO:0048147; P:negative regulation of fibroblast proliferation; IEA:Ensembl.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0035563; P:positive regulation of chromatin binding; IEA:Ensembl.
DR GO; GO:2001178; P:positive regulation of mediator complex assembly; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 2.40.290.30; -; 1.
DR InterPro; IPR045105; Med25/PTOV1.
DR InterPro; IPR021394; Med25_PTOV.
DR InterPro; IPR038196; Med25_PTOV_sf.
DR InterPro; IPR021406; Mediator_Med25_NR-box.
DR InterPro; IPR021397; Mediator_Med25_SD1.
DR InterPro; IPR021419; Mediator_Med25_VWA.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12433; PTHR12433; 1.
DR Pfam; PF11232; Med25; 1.
DR Pfam; PF11244; Med25_NR-box; 1.
DR Pfam; PF11235; Med25_SD1; 1.
DR Pfam; PF11265; Med25_VWA; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
PE 2: Evidence at transcript level;
KW Activator; Methylation; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..746
FT /note="Mediator of RNA polymerase II transcription subunit
FT 25"
FT /id="PRO_0000304951"
FT REGION 1..226
FT /note="Interaction with the Mediator complex"
FT /evidence="ECO:0000250"
FT REGION 233..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 298..390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 389..543
FT /note="Interaction with VP16"
FT /evidence="ECO:0000250"
FT REGION 395..545
FT /note="Interaction with CREBBP"
FT /evidence="ECO:0000250"
FT REGION 548..746
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 563..652
FT /note="Interaction with RARA"
FT /evidence="ECO:0000250"
FT REGION 639..706
FT /note="Interaction with RARA"
FT /evidence="ECO:0000250"
FT MOTIF 645..649
FT /note="LXXLL motif"
FT COMPBIAS 306..324
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 325..345
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..601
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 607..637
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 648..705
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 724
FT /note="Asymmetric dimethylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q8VCB2"
SQ SEQUENCE 746 AA; 78110 MW; F2F43D6AE943496A CRC64;
MVPGSEGPAR AGGLVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD
YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST
ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTETLV QKIGERGIYF
SIVSPRKLPA LRLLFEKAAP PAMLEPLQPP ADVSQDPRHM VLVRGLVLPV GGGSAPGPLQ
PKQPVPLPPA APAGATLSTA PQQPLPPVPQ QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG
PRFSPINPLQ QATPGVGPPY SQTQATQLPP GPPGAPKPPP ASQPSLVSTV APGPGLAPPA
QPGAPSMAGT VAPGGVSGPS PAQLGAPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN
TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL
DSLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV
ITNHKQVQQQ KLEQQRGMGA QQAPPGLGPI LEDQARPSQN LLQLRPPQPQ PQGTVGASAA
AGQPQPQGAA PAPPGAPQGP PGAAPGPPPP GPLLRPQNPG ANPQLRSLLL NPPPPQTGVP
PPQASLHHLQ PPGAPALLPP PHQGLGQPQL GPPLLHPPPA QSWPAQLPPR ASLPGQMLLS
GGPRGPVPQP GLQPSVMEDD ILMDLI
