ID   MED25_DANRE             Reviewed;         701 AA.
AC   Q6PEH8;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 25;
DE   AltName: Full=Mediator complex subunit 25;
GN   Name=med25;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 25 family.
CC       {ECO:0000305}.
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DR   EMBL; BC058057; AAH58057.1; -; mRNA.
DR   RefSeq; NP_999931.1; NM_214766.2.
DR   AlphaFoldDB; Q6PEH8; -.
DR   SMR; Q6PEH8; -.
DR   STRING; 7955.ENSDARP00000055392; -.
DR   PaxDb; Q6PEH8; -.
DR   GeneID; 406793; -.
DR   KEGG; dre:406793; -.
DR   CTD; 81857; -.
DR   ZFIN; ZDB-GENE-040426-2850; med25.
DR   eggNOG; ENOG502QRN5; Eukaryota.
DR   InParanoid; Q6PEH8; -.
DR   OrthoDB; 340324at2759; -.
DR   PhylomeDB; Q6PEH8; -.
DR   Reactome; R-DRE-212436; Generic Transcription Pathway.
DR   PRO; PR:Q6PEH8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0060021; P:roof of mouth development; IMP:ZFIN.
DR   Gene3D; 2.40.290.30; -; 1.
DR   Gene3D; 3.40.50.410; -; 1.
DR   InterPro; IPR045105; Med25/PTOV1.
DR   InterPro; IPR021394; Med25_PTOV.
DR   InterPro; IPR038196; Med25_PTOV_sf.
DR   InterPro; IPR021406; Mediator_Med25_NR-box.
DR   InterPro; IPR021419; Mediator_Med25_VWA.
DR   InterPro; IPR002035; VWF_A.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   PANTHER; PTHR12433; PTHR12433; 1.
DR   Pfam; PF11232; Med25; 1.
DR   Pfam; PF11244; Med25_NR-box; 1.
DR   Pfam; PF11265; Med25_VWA; 1.
DR   SUPFAM; SSF53300; SSF53300; 1.
DR   PROSITE; PS50234; VWFA; 1.
PE   2: Evidence at transcript level;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..701
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   25"
FT                   /id="PRO_0000304954"
FT   REGION          277..388
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          580..617
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          650..689
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           621..625
FT                   /note="LXXLL motif"
FT   COMPBIAS        283..302
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        310..366
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        367..381
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        580..603
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   701 AA;  75463 MW;  07153C0E499D04FE CRC64;
     MDPTSKSGLN QVSDVVFVIE GTANLGPYFE SLRNNYILPT IEYFNGGPPA ETDFGGDYGG
     TQYGLVVFNT VDCAPESYVQ CHAPTSSAFE FVSWIDSIQF MGGGAESCSL IAEGLSVALQ
     LFDDFKKMRE QIGQTHKVCV LLCNSPPYLL PAVESVSYTG CTADSLVQII RDRGIHFSVI
     APRKLPALRS LFERASSVAG LPESSHPDYS QDPFHMVLVR GIALPVAPGG GSVPVSLKPV
     LPPQSLPVNQ NPLVPVASTA PQMNTFQNVS LHAAHDAAQK ALEEAANQQQ KNRFGQISTP
     PFSQSPVLPP GGKPSLSTVT TVSPPVLTQQ QVPPQQPQQQ TQVPQPGLPA VNQQPPQASQ
     QQPNNQQTPP PTQPGMAAVP APPPGAQQGV ANKVVAWSGV LEWQEKPKAS SMDSNTKLTR
     SLPCQVQVSQ GENLNTDQWP QKLIMQLIPQ QLLTTLGPLF RNSRMVQFLF TNKDMESLKG
     LFRIMTSGFA GCVHFPHSAP CEVRVLMLLY SSRKRIFMGL IPNDQSGFVN GIRQVITNHK
     QVQQQRSMQP GQVQPNQNFL NRPPGPIPVT HGNVPQQLRA AATGNQQAPV TGAPPNQVQG
     QAQAPGGGML RLQNPGANPQ LRSLLLSQQP QGGMQGMQGM HPIQQMVHAA PGGGAQMQPQ
     WRQPHQGPLM VPTGPRGPVT QNPGMPSVSS VMEDEILMDL I