MED25_DROME
ID MED25_DROME Reviewed; 863 AA.
AC Q9VDR1;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 25;
DE AltName: Full=Mediator complex subunit 25;
DE AltName: Full=dmPTOV2;
GN Name=MED25; Synonyms=Arc92; ORFNames=CG12254;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC activated transcription of the MtnA, MtnB and MtnD genes. {ECO:0000250,
CC ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 25 family.
CC {ECO:0000305}.
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DR EMBL; AE014297; AAF55729.1; -; Genomic_DNA.
DR EMBL; AY069370; AAL39515.1; -; mRNA.
DR RefSeq; NP_650854.1; NM_142597.4.
DR AlphaFoldDB; Q9VDR1; -.
DR SMR; Q9VDR1; -.
DR BioGRID; 67371; 15.
DR IntAct; Q9VDR1; 3.
DR STRING; 7227.FBpp0083234; -.
DR PaxDb; Q9VDR1; -.
DR PRIDE; Q9VDR1; -.
DR EnsemblMetazoa; FBtr0083825; FBpp0083234; FBgn0038760.
DR GeneID; 42385; -.
DR KEGG; dme:Dmel_CG12254; -.
DR CTD; 81857; -.
DR FlyBase; FBgn0038760; MED25.
DR VEuPathDB; VectorBase:FBgn0038760; -.
DR eggNOG; ENOG502QRN5; Eukaryota.
DR GeneTree; ENSGT00940000168585; -.
DR HOGENOM; CLU_007594_1_1_1; -.
DR InParanoid; Q9VDR1; -.
DR OMA; NDQQKIP; -.
DR OrthoDB; 540705at2759; -.
DR PhylomeDB; Q9VDR1; -.
DR BioGRID-ORCS; 42385; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 42385; -.
DR PRO; PR:Q9VDR1; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038760; Expressed in eye disc (Drosophila) and 22 other tissues.
DR Genevisible; Q9VDR1; DM.
DR GO; GO:0016592; C:mediator complex; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISS:FlyBase.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IMP:FlyBase.
DR GO; GO:0006963; P:positive regulation of antibacterial peptide biosynthetic process; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR Gene3D; 2.40.290.30; -; 1.
DR InterPro; IPR045105; Med25/PTOV1.
DR InterPro; IPR021394; Med25_PTOV.
DR InterPro; IPR038196; Med25_PTOV_sf.
DR InterPro; IPR021419; Mediator_Med25_VWA.
DR PANTHER; PTHR12433; PTHR12433; 2.
DR Pfam; PF11232; Med25; 1.
DR Pfam; PF11265; Med25_VWA; 1.
PE 2: Evidence at transcript level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..863
FT /note="Mediator of RNA polymerase II transcription subunit
FT 25"
FT /id="PRO_0000304957"
FT REGION 224..408
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 456..520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 669..743
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 817..821
FT /note="LXXLL motif"
FT COMPBIAS 231..356
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 377..408
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 669..725
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 863 AA; 96642 MW; 4CE3AC9532892461 CRC64;
MEVDQIPLAD VVFVIEGSAI NGAYINELKT NYILPTLEHF TTGSIDEREY LIAERFATLY
GIVVYRTAAN LLEPVCSTYG PFLQPQKVME TIERLPLVGG GMESCAHMAE GFAAAHGCFD
DISERRQLLD QTSVQRHCIL ICNSPPYQMP TTESWKYPGK SCEQLAALFN ERKINLSIIA
PRKMPVLFKL FMKADGDQPI TSKNYAKNIR HLVLLKGYSL KERAPSPNSM AAQMAAPNAA
QATVQQQQQQ QQNQAGQQQQ GQGMPMDTTP AQQQQQQQQQ QQQQQQQGNP QQQVMNMNTM
QQQQPGPNPP AGLLNPQQQQ QLLQQQQQNQ FVSNQMQNQN FQQNVGPGQN RWMYPNQPGQ
ARPPFMQGAG NVGGVGQGGG MQQNPNSALI SRINAPPPNQ TVTSLQQQQQ QQAQQQQQQQ
QQAQQQQQQR MQMLSQQQML NHQQLQQQQQ LAQQQQQQQQ GQQQQQVNPN AGNNMMPASN
AGNMSNPQQQ QQQVGQQSNP QQQGNPQQQQ QGNSQQEQAS LREKIWTGVL EWSEKPKSDQ
QKIPHTLQCT VCTNIKDGEP EIKAENWPPK LLMQLMPKHL VGNIGGQFLK DSKMVVFRPT
PGEALDSLAK MMTSGYAGCV HFSSIPNSPA CDLKVLILLY TPDRNAFLGF IPNNQAMFVE
RLRKVIQQKQ HGNMQQQQQQ QQMMQQQGKS PMELQQQQQQ QQQQQQMQQD NSQQQHYNQF
QLNMQMGGGG PGGGPGPGPG GMPMQQNQMQ MNMMQQQRMP LGVGVGVGVG PGGVPNPNLQ
QQLQQVAPNV AAMQQQQAQQ QQQRMVRPMM SNNNPGLRQL LQHQTTPGNQ FRPQMGGQNP
NQMGAGGPMV GNRNFDDGNY EFM
