ARH38_MOUSE
ID ARH38_MOUSE Reviewed; 770 AA.
AC Q80VK6; E2QRQ2; Q9D274;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Rho guanine nucleotide exchange factor 38;
GN Name=Arhgef38;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Cecum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-34, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May act as a guanine-nucleotide releasing factor.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=3;
CC IsoId=Q80VK6-3; Sequence=Displayed;
CC Name=1;
CC IsoId=Q80VK6-1; Sequence=VSP_053831;
CC Name=2;
CC IsoId=Q80VK6-2; Sequence=VSP_031334, VSP_031335;
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DR EMBL; AK020284; BAB32054.1; -; mRNA.
DR EMBL; AC124593; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC124605; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC049886; AAH49886.1; -; mRNA.
DR CCDS; CCDS17849.1; -. [Q80VK6-1]
DR CCDS; CCDS89690.1; -. [Q80VK6-3]
DR RefSeq; NP_084229.1; NM_029953.1. [Q80VK6-1]
DR AlphaFoldDB; Q80VK6; -.
DR SMR; Q80VK6; -.
DR STRING; 10090.ENSMUSP00000114238; -.
DR iPTMnet; Q80VK6; -.
DR PhosphoSitePlus; Q80VK6; -.
DR MaxQB; Q80VK6; -.
DR PaxDb; Q80VK6; -.
DR PeptideAtlas; Q80VK6; -.
DR PRIDE; Q80VK6; -.
DR ProteomicsDB; 283202; -. [Q80VK6-3]
DR ProteomicsDB; 283203; -. [Q80VK6-1]
DR ProteomicsDB; 283204; -. [Q80VK6-2]
DR Antibodypedia; 50147; 11 antibodies from 6 providers.
DR DNASU; 77669; -.
DR Ensembl; ENSMUST00000147041; ENSMUSP00000114238; ENSMUSG00000040969. [Q80VK6-3]
DR Ensembl; ENSMUST00000161022; ENSMUSP00000124280; ENSMUSG00000040969. [Q80VK6-1]
DR GeneID; 77669; -.
DR KEGG; mmu:77669; -.
DR UCSC; uc008rkl.1; mouse. [Q80VK6-1]
DR CTD; 54848; -.
DR MGI; MGI:1924919; Arhgef38.
DR VEuPathDB; HostDB:ENSMUSG00000040969; -.
DR eggNOG; KOG3519; Eukaryota.
DR GeneTree; ENSGT00950000183088; -.
DR HOGENOM; CLU_005159_1_0_1; -.
DR InParanoid; Q80VK6; -.
DR OMA; KQHFTAC; -.
DR OrthoDB; 207472at2759; -.
DR PhylomeDB; Q80VK6; -.
DR TreeFam; TF317245; -.
DR Reactome; R-MMU-193648; NRAGE signals death through JNK.
DR Reactome; R-MMU-416482; G alpha (12/13) signalling events.
DR BioGRID-ORCS; 77669; 0 hits in 59 CRISPR screens.
DR ChiTaRS; Arhgef38; mouse.
DR PRO; PR:Q80VK6; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q80VK6; protein.
DR Bgee; ENSMUSG00000040969; Expressed in right kidney and 16 other tissues.
DR ExpressionAtlas; Q80VK6; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.1270.60; -; 1.
DR Gene3D; 1.20.900.10; -; 1.
DR InterPro; IPR027267; AH/BAR_dom_sf.
DR InterPro; IPR004148; BAR_dom.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR036028; SH3-like_dom_sf.
DR InterPro; IPR001452; SH3_domain.
DR Pfam; PF03114; BAR; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR Pfam; PF14604; SH3_9; 1.
DR SMART; SM00721; BAR; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SMART; SM00326; SH3; 2.
DR SUPFAM; SSF103657; SSF103657; 1.
DR SUPFAM; SSF48065; SSF48065; 1.
DR SUPFAM; SSF50044; SSF50044; 2.
DR PROSITE; PS51021; BAR; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50002; SH3; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Guanine-nucleotide releasing factor;
KW Phosphoprotein; Reference proteome; Repeat; SH3 domain.
FT CHAIN 1..770
FT /note="Rho guanine nucleotide exchange factor 38"
FT /id="PRO_0000318988"
FT DOMAIN 94..285
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT DOMAIN 327..542
FT /note="BAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00361"
FT DOMAIN 581..644
FT /note="SH3 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT DOMAIN 706..769
FT /note="SH3 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT REGION 33..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..83
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 34
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 171..175
FT /note="EVFLQ -> GNYAK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031334"
FT VAR_SEQ 176..770
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031335"
FT VAR_SEQ 220..770
FT /note="Missing (in isoform 1)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_053831"
FT CONFLICT 143
FT /note="S -> F (in Ref. 1; BAB32054)"
FT /evidence="ECO:0000305"
FT CONFLICT 145
FT /note="H -> R (in Ref. 1; BAB32054)"
FT /evidence="ECO:0000305"
FT CONFLICT 161
FT /note="D -> Y (in Ref. 1; BAB32054)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 770 AA; 87679 MW; AD271F09ACCA2147 CRC64;
MEPREAAGKE TMGSKKKNLT FLRSRLYMLE RRKTDTVVDS SVSGDHSGSL RRSQSDRTEY
NQKLQEKMTP QAECSSAETP TPEDEQQVGR MMAKRAKIIR ELIQTERDYL TDLELCLREV
VQPLRSKQID RLDVEGLFSN IESVHQISAK LLSLLEEATT DVEPAMQVIG EVFLQIKGPL
EDIYKIYCYH HDEAHSVLES YEKEEELKQH LSHCLQSLKN IYLQEGKPNL LDMGSLMIKP
IQRVMKYPLL LCELRNSTPP SHPDYRALGE AFAAVKDINV NINELKRRKD LVLKYKKNEE
EESLKDKLSK LNIHSISKKS KRVTNHLKIL TRGESQVKDN TFNREEKLFR SLEKTVRLCV
KNISSCLQHI EEATPFTLQS GAELREISYQ DGEKNGTEPQ DQASKPYQDF AARSQRLILN
PLSALLSLFP GPQKLIQKRY DKLLDYNSSL PRSATTESDL AKREYEALNA QLVEELQAFN
QAAKTILLNC LCSFVTLLRD LMQVALQAYS TVKTVPLPVL GISEIQSRVL EEVHSLTFVK
ENSATFIERK LSFEKKKPAQ ILPEVPHQTD AHRSKLLSTY GAEELYRAKR KCNATQEHDI
NLLEGELVAV LEQKDPLGST SRWFVDTGCV KGYVYSSFLK PHNPGKGQKV DADNRFCDDD
FENISLFVSC RPAGDRVSIS DSSSSLSGSC GKFETNGADA DNFQEVDEQI FYAVHAFQAR
SDHELSLQEY QRVHILRFCD LSGNKEWWLA EAQGQKGYVP ANYLGKMTYA
