ID   MED25_SOLLC             Reviewed;         805 AA.
AC   A0A3Q7JC00;
DT   03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT   10-APR-2019, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 25 {ECO:0000305};
GN   Name=MED25 {ECO:0000303|PubMed:30610166};
GN   OrderedLocusNames=Solyc12g070100 {ECO:0000305};
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Heinz 1706;
RX   PubMed=22660326; DOI=10.1038/nature11119;
RG   Tomato Genome Consortium;
RT   "The tomato genome sequence provides insights into fleshy fruit
RT   evolution.";
RL   Nature 485:635-641(2012).
RN   [2]
RP   FUNCTION, AND INTERACTION WITH MYC2.
RX   PubMed=30610166; DOI=10.1105/tpc.18.00405;
RA   Liu Y., Du M., Deng L., Shen J., Fang M., Chen Q., Lu Y., Wang Q., Li C.,
RA   Zhai Q.;
RT   "MYC2 regulates the termination of jasmonate signaling via an
RT   autoregulatory negative feedback loop.";
RL   Plant Cell 31:106-127(2019).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional pre-initiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). Plays a positive
CC       role in wound-induced activation of jasmonate-responsive genes whose
CC       promoters are targeted by MYC2 (PubMed:30610166).
CC       {ECO:0000250|UniProtKB:Q7XYY2, ECO:0000269|PubMed:30610166}.
CC   -!- SUBUNIT: Interacts with MYC2 (via N-terminus) (PubMed:30610166). MED25
CC       competes with JAZ7 for binding to MYC2 (PubMed:30610166).
CC       {ECO:0000269|PubMed:30610166}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 25 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CM001075; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_004252687.1; XM_004252639.3.
DR   AlphaFoldDB; A0A3Q7JC00; -.
DR   SMR; A0A3Q7JC00; -.
DR   STRING; 4081.Solyc12g070100.1.1; -.
DR   EnsemblPlants; Solyc12g070100.2.1; Solyc12g070100.2.1; Solyc12g070100.2.
DR   GeneID; 101258928; -.
DR   Gramene; Solyc12g070100.2.1; Solyc12g070100.2.1; Solyc12g070100.2.
DR   KEGG; sly:101258928; -.
DR   OMA; TPGMTQQ; -.
DR   Proteomes; UP000004994; Chromosome 12.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR045105; Med25/PTOV1.
DR   InterPro; IPR021419; Mediator_Med25_VWA.
DR   PANTHER; PTHR12433; PTHR12433; 1.
DR   Pfam; PF11265; Med25_VWA; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..805
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   25"
FT                   /id="PRO_0000447558"
FT   REGION          430..455
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          786..805
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   805 AA;  86438 MW;  ED1CC7E56852E138 CRC64;
     MVDKLIVAVE GTAVLGPYWK IIVSDYLDKI IRCFFGVDST SQKSSAADVE VSMVMFNTHG
     PYSACLVQRS GWTKDMDTFL QWLSAIPFSG GGFNDAAVAE GLAEALVMFS VPNGNQTQQK
     MEGKKHCILI SGSNPYPLPT PVYRPQMQKL EQNENIEAQT DSRLADAETV AKTFPQCSIS
     LSVICPKKLP KLRAIYDAGK HNPRAADPPI DTAKNPNFLV LISENFIEAR AAFSRSGLTN
     LASNHSPVKM DVSSVLPVSG TQSISNSAAN VSVISRPPIS AGNIPPATVK IEPNTVTPMT
     GPGFSHIPSV RPALQPVPSL QASSPLSVSQ EMVSHTENVQ EMKPIVSGMT QSLRPVAAAA
     ANVKILNGVA QAHQVLGGGT SIGLQSMGGT PMLSSMISSG MASSVPASQA VLSSGQSGVT
     TMTGAVPLAG SAQNTQNSAP SSFTSTAPSM SGQTVPAMSQ GNIPGTQMMP SGTGMNQNML
     TGLGATGLPS GTGTMMPTPG MSQQGQPGMQ PVGVNSTSAN MPLSQQQTSG ALPSAQSKYV
     KVWEGNLSGQ RQGQPVFITR LEGYRSASAS ESLAANWPPT MQIVRLISQD HMNNKQYVGK
     ADFLVFRAMN QHGFLSQLQE KKLCAVIQLP SQTLLLSVSD KACRLIGMLF PGDMVVFKPQ
     IPSQQQQQQQ QQQLQAQHPQ LQQQQQQQQQ HLTQLQQQPL QQLQQQQQQQ PLMQLQQQQQ
     IPLQQSQVPQ MQQQQIHQMQ QQQQIPQMQQ QQQIPQMQQQ QQQQPMVGTG MNQTYMQGPA
     RSQLMSQSQG SSQGLPITPG GGFMN