ID   MED25_XENLA             Reviewed;         801 AA.
AC   Q6GP15;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 25;
DE   AltName: Full=Mediator complex subunit 25;
GN   Name=med25;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Spleen;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 25 family.
CC       {ECO:0000305}.
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DR   EMBL; BC073333; AAH73333.1; -; mRNA.
DR   RefSeq; NP_001085778.1; NM_001092309.1.
DR   RefSeq; XP_018082410.1; XM_018226921.1.
DR   AlphaFoldDB; Q6GP15; -.
DR   SMR; Q6GP15; -.
DR   DNASU; 444205; -.
DR   GeneID; 444205; -.
DR   KEGG; xla:444205; -.
DR   CTD; 444205; -.
DR   Xenbase; XB-GENE-5831033; med25.S.
DR   OMA; FRNQAPQ; -.
DR   OrthoDB; 340324at2759; -.
DR   Proteomes; UP000186698; Chromosome 7S.
DR   Bgee; 444205; Expressed in ovary and 19 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.40.290.30; -; 1.
DR   InterPro; IPR045105; Med25/PTOV1.
DR   InterPro; IPR021394; Med25_PTOV.
DR   InterPro; IPR038196; Med25_PTOV_sf.
DR   InterPro; IPR021406; Mediator_Med25_NR-box.
DR   InterPro; IPR021397; Mediator_Med25_SD1.
DR   InterPro; IPR021419; Mediator_Med25_VWA.
DR   InterPro; IPR002035; VWF_A.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   PANTHER; PTHR12433; PTHR12433; 1.
DR   Pfam; PF11232; Med25; 1.
DR   Pfam; PF11244; Med25_NR-box; 1.
DR   Pfam; PF11235; Med25_SD1; 1.
DR   Pfam; PF11265; Med25_VWA; 1.
DR   SUPFAM; SSF53300; SSF53300; 1.
DR   PROSITE; PS50234; VWFA; 1.
PE   2: Evidence at transcript level;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..801
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   25"
FT                   /id="PRO_0000304955"
FT   REGION          647..735
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           689..693
FT                   /note="LXXLL motif"
FT   COMPBIAS        647..677
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        684..735
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   801 AA;  86512 MW;  255518A1935C4E9F CRC64;
     MDAATPGNGI ISDVVFVIEG TANLGPYFES LRKHYLLPAI EYFNGGPPAE TDFGGDYGGT
     QYSLVVFNTV DCAPESYVQC HAPTSSAYEF VQWLDSIKFM GGGGESCSLI AEGLSTALQL
     FDDFKKMREQ IGQTHKVCIL ICNSPPYLLP AVESTTYSGY TTENLVQKIG ERGIHFSIIS
     PRKLPALRTL FEKAMPVGLI EPQPKDYSQD PRHMILVRGM VLPVGGATSV PGVMPPKQHI
     SQPPLPVVPP QIASAPSHQL PPVQPPYMQV PQQSTLTPAH AAAHSAVEAA KNQKNSPSNR
     FLLPNLNPMP NAPAVGTPFN QPPAPTLPPN ASVPKMVPSP SSLMTPASQS SLITTVTTGP
     GPAPVQLQQQ GAPQQPVPPS MPITGAVGGV QAPQPSQPQI GTVQLPCTQT PVNGNKLLAW
     SGVLEWQEKP RSVSVDNNAK LTRSLPCQVY INPGENLKTD QWPQKLIMQL IPQQLLTTLG
     PLFRNSRMVQ FHFTNKDLES LKGLYRIMGS GFAGCVHFPH TTPCEVRVLM LLYSSKKKIF
     MGLIPNDQSG FVNGIRQVIT NHKQVQMQKI DQQRNMGAVQ GVGAGSVPAN SQGFLQKQPG
     TLPVGQAVSQ QMQGQQVAPG MPSISQVAMM DEQQRSQNLL HIRVQQPQQL ASQAPPQATQ
     SSVQAPGQPQ NPQPGAMLRP QNPGANPQLR NLLLSQQPPQ SSVPQTQQPL HHMQQAAQSM
     LPHQAMGQQM QHQAPGQLQL PGQSLMHQTP AQQWGNQMQQ RAPIPGTLMM SAGPRGPVPQ
     SGLQQVQAQS VMEDDILMDL I