ID   MED25_XENTR             Reviewed;         805 AA.
AC   A4IHD9;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 25;
DE   AltName: Full=Mediator complex subunit 25;
GN   Name=med25;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 25 family.
CC       {ECO:0000305}.
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DR   EMBL; BC135482; AAI35483.1; -; mRNA.
DR   RefSeq; NP_001096266.1; NM_001102796.1.
DR   AlphaFoldDB; A4IHD9; -.
DR   SMR; A4IHD9; -.
DR   STRING; 8364.ENSXETP00000000590; -.
DR   PaxDb; A4IHD9; -.
DR   DNASU; 100124831; -.
DR   GeneID; 100124831; -.
DR   KEGG; xtr:100124831; -.
DR   CTD; 81857; -.
DR   Xenbase; XB-GENE-5830877; med25.
DR   eggNOG; ENOG502QRN5; Eukaryota.
DR   HOGENOM; CLU_007594_0_0_1; -.
DR   InParanoid; A4IHD9; -.
DR   OMA; NDQQKIP; -.
DR   OrthoDB; 340324at2759; -.
DR   PhylomeDB; A4IHD9; -.
DR   TreeFam; TF329598; -.
DR   Proteomes; UP000008143; Chromosome 7.
DR   Proteomes; UP000790000; Unplaced.
DR   ExpressionAtlas; A4IHD9; baseline and differential.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 2.40.290.30; -; 1.
DR   InterPro; IPR045105; Med25/PTOV1.
DR   InterPro; IPR021394; Med25_PTOV.
DR   InterPro; IPR038196; Med25_PTOV_sf.
DR   InterPro; IPR021406; Mediator_Med25_NR-box.
DR   InterPro; IPR021397; Mediator_Med25_SD1.
DR   InterPro; IPR021419; Mediator_Med25_VWA.
DR   InterPro; IPR002035; VWF_A.
DR   InterPro; IPR036465; vWFA_dom_sf.
DR   PANTHER; PTHR12433; PTHR12433; 1.
DR   Pfam; PF11232; Med25; 1.
DR   Pfam; PF11244; Med25_NR-box; 1.
DR   Pfam; PF11235; Med25_SD1; 1.
DR   Pfam; PF11265; Med25_VWA; 1.
DR   SUPFAM; SSF53300; SSF53300; 1.
DR   PROSITE; PS50234; VWFA; 1.
PE   2: Evidence at transcript level;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..805
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   25"
FT                   /id="PRO_0000304956"
FT   REGION          308..332
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          647..691
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           691..695
FT                   /note="LXXLL motif"
FT   COMPBIAS        317..331
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        647..679
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   805 AA;  87144 MW;  38C28F1279F9A1CB CRC64;
     MDAATPGNGG IISDVVFVIE GTANLGPYFE SLRKHYLLPA IEYFNGGPPA ETDFGGDYGG
     TQYSLVVFNT VDCAPESYVQ CHAPTSSAYE FVQWLDSIRF MGGGGESCSL IAEGLSTALQ
     LFDDFKKMRE QIGQTHKVCI LICNSPPYLL PAVESTTYSG YTTENLVQKI GERGIHFSVI
     SPRKLPALRT LFEKAMPVGL IEPQPKDYSQ DPRHMILVRG MVLPVGGATS VPGVIPPKQP
     ISQPPLPVVP PQIANAPSHQ LPPVQPPYMQ VPQQNTLTTA HAAAQSAVEA AKNQKNNLPN
     RFPLPNLNQM PPTSAVGTPF NQPPPPAMPQ NTSVPKMVTS TASLMTPASQ PSLVTTVTTG
     PGPAPVQLQQ QGAQQQPVPP SMPITGAAGG VQAPQPSQPQ IGTAQLPCTQ TPVNGNKLLA
     WSGVLEWQEK PRSVSVDNNA KLTRSLPCQV YVNPGENLKT DQWPQKLIMQ LIPQQLLTTL
     GPLFRNSRMV QFHFTNKDLE SLKGLYRIMG SGFQAGCVHF PHTTPCEVRV LMLLYSSKKK
     IFMGLIPNDQ SGFVNGIRQV ITNHKQVQMQ KIDQQRNMGA GQAVGTGNVP ANTQAFLQKQ
     PGALPVAQAV PQQMQGQQVA PGMPSISQVT MMDEQQRTQN LLHIRVQQPQ QAASQAPPQA
     TQTTVQAPGQ PQNPQPGAML RPQNPGANPQ LRNLLLSQQP PQTSVPQTQQ PLHHMQQAAQ
     GMLPHQAMGQ QMQHQAPGQQ QLQLPGQTLM HQAPAQQWGN QMQQRAPIPG TLMMSAGPRG
     PVPQQGLQQV QAQSVMEDDI LMDLI