MED26_DROME
ID MED26_DROME Reviewed; 1483 AA.
AC Q9V4F9; Q6AWG8; Q86P16; Q8SX84;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 26;
DE AltName: Full=Mediator complex subunit 26;
DE AltName: Full=dARC70;
GN Name=MED26; Synonyms=Arc70; ORFNames=CG1793, CG1823;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 680-1483.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-204; SER-258; SER-421;
RP THR-541; SER-551; SER-1177 AND THR-1179, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC activated transcription of the MtnA gene. {ECO:0000250,
CC ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex (By similarity). Interacts
CC with MED6 and MED17. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 26 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM11139.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAO39538.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014135; AAF59314.2; -; Genomic_DNA.
DR EMBL; AE014135; AAF59315.2; -; Genomic_DNA.
DR EMBL; BT003534; AAO39538.1; ALT_SEQ; mRNA.
DR EMBL; BT015280; AAT94509.1; ALT_TERM; mRNA.
DR EMBL; AY094786; AAM11139.1; ALT_INIT; mRNA.
DR RefSeq; NP_651936.1; NM_143679.4.
DR RefSeq; NP_726610.1; NM_166791.2.
DR AlphaFoldDB; Q9V4F9; -.
DR SMR; Q9V4F9; -.
DR BioGRID; 68645; 14.
DR IntAct; Q9V4F9; 5.
DR STRING; 7227.FBpp0088313; -.
DR iPTMnet; Q9V4F9; -.
DR PaxDb; Q9V4F9; -.
DR EnsemblMetazoa; FBtr0089253; FBpp0088313; FBgn0039923.
DR EnsemblMetazoa; FBtr0089254; FBpp0088314; FBgn0039923.
DR GeneID; 43816; -.
DR KEGG; dme:Dmel_CG1793; -.
DR CTD; 9441; -.
DR FlyBase; FBgn0039923; MED26.
DR VEuPathDB; VectorBase:FBgn0039923; -.
DR eggNOG; ENOG502S9CY; Eukaryota.
DR GeneTree; ENSGT00390000000259; -.
DR HOGENOM; CLU_249548_0_0_1; -.
DR InParanoid; Q9V4F9; -.
DR OMA; NCDDVNN; -.
DR OrthoDB; 246541at2759; -.
DR PhylomeDB; Q9V4F9; -.
DR SignaLink; Q9V4F9; -.
DR BioGRID-ORCS; 43816; 0 hits in 1 CRISPR screen.
DR ChiTaRS; MED26; fly.
DR GenomeRNAi; 43816; -.
DR PRO; PR:Q9V4F9; -.
DR Proteomes; UP000000803; Chromosome 4.
DR Bgee; FBgn0039923; Expressed in cleaving embryo and 31 other tissues.
DR Genevisible; Q9V4F9; DM.
DR GO; GO:0000785; C:chromatin; IDA:FlyBase.
DR GO; GO:0070847; C:core mediator complex; IPI:FlyBase.
DR GO; GO:0016592; C:mediator complex; ISS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IDA:FlyBase.
DR GO; GO:0005721; C:pericentric heterochromatin; IPI:FlyBase.
DR GO; GO:0005701; C:polytene chromosome chromocenter; IDA:FlyBase.
DR GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR Gene3D; 1.20.930.10; -; 1.
DR InterPro; IPR042376; MED26.
DR InterPro; IPR003617; TFIIS/CRSP70_N_sub.
DR InterPro; IPR035441; TFIIS/LEDGF_dom_sf.
DR InterPro; IPR017923; TFIIS_N.
DR PANTHER; PTHR15201; PTHR15201; 1.
DR Pfam; PF08711; Med26; 1.
DR SMART; SM00509; TFS2N; 1.
DR SUPFAM; SSF47676; SSF47676; 1.
DR PROSITE; PS51319; TFIIS_N; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1483
FT /note="Mediator of RNA polymerase II transcription subunit
FT 26"
FT /id="PRO_0000304962"
FT DOMAIN 8..85
FT /note="TFIIS N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00649"
FT REGION 227..278
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 414..438
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 480..518
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 541..575
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 989..1041
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 227..242
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 561..575
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 989..1014
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 204
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 258
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 421
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 541
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 551
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1177
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 1179
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 597
FT /note="L -> V (in Ref. 3; AAO39538)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1483 AA; 165516 MW; A84C676B3806E4D1 CRC64;
MNQIEIQELT THLSQALDQN YDVVNMDAVL CVICALEGTT ITKEQLEATR LAKYINQLRR
RTKNEHLARR AKSLLKKWRE MVGIQQTATE NLAHPSQISS SQPALDLVKS PITSFITEPI
APSQQIVSDL HSNIDSAEPP LSGEHTLLHP NFSNLVNSIK DSDRHENIAI TTLHTHKDRR
HSHSIRSCHV ASPQPIVIDH SVNSVINLTD DSTVKINEAS VVIDIASDSD ENDNNGSLNP
KKLNSIVPAP LPIPSTPSSR QRKLKKEKRS KEREGQVATN TRFGAKASDG FQQAALTTDS
EIFSLSNSSM SSIVSGDATS SYSQNKSRLN SNELTFTGRF KAVDHLFRST ESGKHKIDEY
SAYDSNASCS RLSPSTVDEV KKAEHLFDAQ LTNTTANQIP MSAVSIGYES NTRHEYLESD
SPSQIPKRRG RKKGSKGVDA LIAKESSSLS QQIFFGGSTV KKVKTTKELF NEIQSRKLSV
SMQSSASNLS NSSTNRDLPS HTTFPRQTSS CSDTSMNSPH ILETLSGSAI FASKIDDLGN
TDSDTVTSDP SHDSNKSQEI KECTSLDSNS NSIQSLPLAN NRENLIPTNV NDITTQLMHL
IHSLNSPLSE IEIERVYQEK IIPCTCIVIE GVQGTLGESN NSTLDEQSYN AKISLNSKDN
LSCHNQDNYS TSQRSVNGEV IDTQPKLVKS IFDLDFDDND DPLYFIMDEI QKPIARADEL
KNNKSDTKNI NFGASAQNAS LNIIHFNDDA QQDNSNQERG KNETQNAIPA FTVHEDPDCL
ARQRFYIQTN KVTSFHINAL HNYYIPNING NWDSVESFTS FQSEHTIMDT MESYTVTNGA
DVVPKYGLLT SDRIRKDLSS LKSIKPYRVK NFTSLISPFL GVAKCLPTCR RARRRFKNFV
NSSTANDKIS NNFESLENMK TSFKNCNPLN VKIDGTTSAI YSHNENHVPM QVNTNVEQLP
PSQSFSSNAI SYNLLKLADD KVCHDGISDK SSNTGCQGNS PYSSSSSSSY SGRKEDQHII
TKNLQNKNIQ LNSRKSDMKR RKRIYLENDS KKKKHYRKRI KTAVNRTFSN LHRNLYSIDS
ENGMKNDYKN EYGNSNNEEY AIVQRPAGDG ENCSNHIVLT IKKTPSKINS PANSMTAFSP
PSTSDGANLQ KSILDLRLED TNISQSITEI PQKKDLSKTD RIVHQSRSSA YRRIFRKPDK
SQTKFIDLDL KHLFHLKANS PCISANAKLH NKLFFPHELS RDNTSGIRER LINYSSSSSS
SYDDDSEVEN TSFLKKANVN KFSASNNRNL KTETETCAAT AISEFKFESD EDSILTSLGD
SDIDIQDEIQ VINDKVDYCN DFQNNKMLES FNSIFVDSLR TNTKNASDNM GSSAHITSLE
SLKPENADAI TRYCNNNNLG VNLSNELSNI SAKPLKSFDS TMKLPSLGIE HCTGIKTSSD
MASTNIAEKK LTRIQQFKEW HQVLQLRSYN NEPLIVLPYV LLE
