MED26_MOUSE
ID MED26_MOUSE Reviewed; 588 AA.
AC Q7TN02; Q8BUP9; Q8R1G7; Q9CS67;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 26;
DE AltName: Full=Cofactor required for Sp1 transcriptional activation subunit 7;
DE Short=CRSP complex subunit 7;
DE AltName: Full=Mediator complex subunit 26;
GN Name=Med26; Synonyms=Crsp7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-435, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP INTERACTION WITH CEBPB.
RX PubMed=20111005; DOI=10.1038/emboj.2010.3;
RA Kowenz-Leutz E., Pless O., Dittmar G., Knoblich M., Leutz A.;
RT "Crosstalk between C/EBPbeta phosphorylation, arginine methylation, and
RT SWI/SNF/Mediator implies an indexing transcription factor code.";
RL EMBO J. 29:1105-1115(2010).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional pre-initiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity). Interacts
CC with CEBPB (when not methylated)(PubMed:20111005).
CC {ECO:0000250|UniProtKB:O95402, ECO:0000269|PubMed:20111005}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 26 family.
CC {ECO:0000305}.
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DR EMBL; AK017726; BAB30898.1; -; mRNA.
DR EMBL; AK083035; BAC38738.1; -; mRNA.
DR EMBL; BC024555; AAH24555.1; -; mRNA.
DR EMBL; BC054737; AAH54737.1; -; mRNA.
DR CCDS; CCDS22416.1; -.
DR RefSeq; NP_081761.2; NM_027485.4.
DR AlphaFoldDB; Q7TN02; -.
DR SMR; Q7TN02; -.
DR BioGRID; 214175; 5.
DR ComplexPortal; CPX-3264; Core mediator complex.
DR IntAct; Q7TN02; 6.
DR MINT; Q7TN02; -.
DR STRING; 10090.ENSMUSP00000058697; -.
DR iPTMnet; Q7TN02; -.
DR PhosphoSitePlus; Q7TN02; -.
DR EPD; Q7TN02; -.
DR jPOST; Q7TN02; -.
DR MaxQB; Q7TN02; -.
DR PaxDb; Q7TN02; -.
DR PeptideAtlas; Q7TN02; -.
DR PRIDE; Q7TN02; -.
DR ProteomicsDB; 293452; -.
DR DNASU; 70625; -.
DR Ensembl; ENSMUST00000058534; ENSMUSP00000058697; ENSMUSG00000045248.
DR GeneID; 70625; -.
DR KEGG; mmu:70625; -.
DR UCSC; uc009mgg.2; mouse.
DR CTD; 9441; -.
DR MGI; MGI:1917875; Med26.
DR VEuPathDB; HostDB:ENSMUSG00000045248; -.
DR eggNOG; KOG1105; Eukaryota.
DR GeneTree; ENSGT00390000000259; -.
DR HOGENOM; CLU_478915_0_0_1; -.
DR InParanoid; Q7TN02; -.
DR OMA; NMVVVLE; -.
DR OrthoDB; 459216at2759; -.
DR PhylomeDB; Q7TN02; -.
DR TreeFam; TF328436; -.
DR Reactome; R-MMU-212436; Generic Transcription Pathway.
DR BioGRID-ORCS; 70625; 35 hits in 74 CRISPR screens.
DR ChiTaRS; Med26; mouse.
DR PRO; PR:Q7TN02; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q7TN02; protein.
DR Bgee; ENSMUSG00000045248; Expressed in seminiferous tubule of testis and 177 other tissues.
DR Genevisible; Q7TN02; MM.
DR GO; GO:0070847; C:core mediator complex; ISO:MGI.
DR GO; GO:0016592; C:mediator complex; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IBA:GO_Central.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR Gene3D; 1.20.930.10; -; 1.
DR InterPro; IPR042376; MED26.
DR InterPro; IPR031416; Med26_C.
DR InterPro; IPR031417; Med26_Mid.
DR InterPro; IPR003617; TFIIS/CRSP70_N_sub.
DR InterPro; IPR035441; TFIIS/LEDGF_dom_sf.
DR InterPro; IPR017923; TFIIS_N.
DR PANTHER; PTHR15201; PTHR15201; 1.
DR Pfam; PF08711; Med26; 1.
DR Pfam; PF15693; Med26_C; 1.
DR Pfam; PF15694; Med26_M; 1.
DR SMART; SM00509; TFS2N; 1.
DR SUPFAM; SSF47676; SSF47676; 1.
DR PROSITE; PS51319; TFIIS_N; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..588
FT /note="Mediator of RNA polymerase II transcription subunit
FT 26"
FT /id="PRO_0000304959"
FT DOMAIN 10..87
FT /note="TFIIS N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00649"
FT REGION 112..393
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 412..441
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..161
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 179..193
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 208..222
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..328
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 375..390
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 435
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 458
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O95402"
FT CONFLICT 64
FT /note="T -> N (in Ref. 1; BAC38738)"
FT /evidence="ECO:0000305"
FT CONFLICT 212..214
FT /note="SDN -> VII (in Ref. 1; BAB30898)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 588 AA; 64680 MW; 0CAFE5930234B293 CRC64;
MTAAPASPQQ MRDRLLQAID SQSNIRNMVA VLEVISSLER YPITKEALEE TRLGKLINDV
RKKTKNEELA KRAKRLLRSW QKLIEPVHQN EVALRALAGA AGSANGGAHN CRPEMGVAGA
PKSIHDLKNR NDIQRLPGQR LDRLGSRKRR GDQRDLGHPG PPHKVSKGSP DPLVPNASPL
PTNGISGSPE SLPSPLDGSG HLGPDGSRLE PSDNEKHSTK IPVNAVRPRP SSPGLGKPPV
PCLQTKAAQL QQLDRADESP GPPYPRGSSR CSFSPRNSRH EGSFSRHRSS YIPKGQVSSP
SPWPQPPDNT QVPSPLPLAQ PPTPPVRRQE LLPNAESPVH WPEQSEGHPR LTGPACRAGF
SPDSSKADSD ATSSGGSDSK KKKRYRPRDY TVNLDGQVAE AGVKPVRLKE RKLTFDPMTR
QIRPLTQKEP VRADSPVPTE QLPRTELEQQ EVKASLQSPF EQTNWKELSR NEIIQSYLSR
QSSLLSSSGA QTPGAHHFMA EYLKQEESSR QGARQPHVLL PLPTPTDLPG LTREVTQDDL
DRIQAQQWPG VNGCEDTQGN WYDWTQCISL DPHGDDGRLN ILPYVCLD
