MED27_ARATH
ID MED27_ARATH Reviewed; 402 AA.
AC Q8RWM3; Q8LFG5; Q9S7S1;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 27;
DE AltName: Full=Mediator of RNA polymerase II transcription subunit 3;
GN Name=MED27; Synonyms=MED3, MED3_1; OrderedLocusNames=At3g09180;
GN ORFNames=F3L24.5, MZB10.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kim C.J., Chen H., Cheuk R., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [7]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 27 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD56333.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF14019.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC009326; AAD56333.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC011436; AAF14019.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74734.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63373.1; -; Genomic_DNA.
DR EMBL; AY093000; AAM12999.1; -; mRNA.
DR EMBL; BT020371; AAV85726.1; -; mRNA.
DR EMBL; AY084863; AAM61426.1; -; mRNA.
DR RefSeq; NP_001325465.1; NM_001337806.1.
DR RefSeq; NP_566345.1; NM_111753.4.
DR AlphaFoldDB; Q8RWM3; -.
DR BioGRID; 5408; 7.
DR IntAct; Q8RWM3; 2.
DR STRING; 3702.AT3G09180.1; -.
DR MetOSite; Q8RWM3; -.
DR PaxDb; Q8RWM3; -.
DR PRIDE; Q8RWM3; -.
DR ProteomicsDB; 238253; -.
DR EnsemblPlants; AT3G09180.1; AT3G09180.1; AT3G09180.
DR EnsemblPlants; AT3G09180.3; AT3G09180.3; AT3G09180.
DR GeneID; 820074; -.
DR Gramene; AT3G09180.1; AT3G09180.1; AT3G09180.
DR Gramene; AT3G09180.3; AT3G09180.3; AT3G09180.
DR KEGG; ath:AT3G09180; -.
DR Araport; AT3G09180; -.
DR TAIR; locus:2083534; AT3G09180.
DR eggNOG; ENOG502QS7K; Eukaryota.
DR HOGENOM; CLU_058530_0_0_1; -.
DR InParanoid; Q8RWM3; -.
DR OMA; FRHITEH; -.
DR OrthoDB; 1256412at2759; -.
DR PhylomeDB; Q8RWM3; -.
DR PRO; PR:Q8RWM3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8RWM3; baseline and differential.
DR Genevisible; Q8RWM3; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR021627; Mediator_Med27.
DR PANTHER; PTHR13130; PTHR13130; 1.
DR Pfam; PF11571; Med27; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..402
FT /note="Mediator of RNA polymerase II transcription subunit
FT 27"
FT /id="PRO_0000418142"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 28..49
FT /evidence="ECO:0000255"
FT COMPBIAS 1..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 11
FT /note="Q -> L (in Ref. 5; AAM61426)"
FT /evidence="ECO:0000305"
FT CONFLICT 283
FT /note="G -> A (in Ref. 5; AAM61426)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 402 AA; 44511 MW; F780151B45FF3A11 CRC64;
MQTLHQSQLL QNPAEAANNQ SESDAPPKQV AQAMERLNQA ARVIADIRLG ADRILEAMFV
ASQPRHTDMP LQLFLREDAS MRQHLQDLRL IGKKLEESGV LTESLRSRSN SWGLHMPLVC
PDGAVVAYAW KRQLAGQAGA SAVDRTRLAL KAFTDQKRRF FPHIDDGLKM EPSSKKHRAS
HLLLENGREE PVDYKTLPDI QSRLEKLVPS VKVSTYGRLN WLKRANSLPG SGSDDPTEAS
KPIFQSSSKL RSGLQTEVVD KIAVIELSFP SLFRAIVSLS PAGSVDPDAV AFFSPDEGGS
YLHARGFSVY HVYKHITEHA ATALQYFLGF GTGTALYSLL LWICSFESVF SKPCTKCGRL
LAMDKKSALI LPPLHRAYQE LPLALNLDVC EAYHSSCSQD DT
