ID   MED27_DANRE             Reviewed;         311 AA.
AC   Q6PFL0;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 27;
DE   AltName: Full=Cofactor required for Sp1 transcriptional activation subunit 8;
DE            Short=CRSP complex subunit 8;
DE   AltName: Full=Mediator complex subunit 27;
GN   Name=med27; Synonyms=crsp34, crsp8;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=16582438; DOI=10.1534/genetics.105.055152;
RA   Duerr K., Holzschuh J., Filippi A., Ettl A.-K., Ryu S., Shepherd I.T.,
RA   Driever W.;
RT   "Differential roles of transcriptional mediator complex subunits
RT   Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 during zebrafish retinal
RT   development.";
RL   Genetics 174:693-705(2006).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). Required for the
CC       development of dopaminergic amacrine cells in the retina. May also
CC       negatively regulate the development of rod photoreceptor cells.
CC       {ECO:0000250, ECO:0000269|PubMed:16582438}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Maternally expressed. Ubiquitously expressed
CC       throughout development. {ECO:0000269|PubMed:16582438}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 27 family.
CC       {ECO:0000305}.
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DR   EMBL; BC057508; AAH57508.1; -; mRNA.
DR   RefSeq; NP_956954.1; NM_200660.1.
DR   AlphaFoldDB; Q6PFL0; -.
DR   SMR; Q6PFL0; -.
DR   STRING; 7955.ENSDARP00000005238; -.
DR   PaxDb; Q6PFL0; -.
DR   Ensembl; ENSDART00000005140; ENSDARP00000005238; ENSDARG00000009681.
DR   GeneID; 393633; -.
DR   KEGG; dre:393633; -.
DR   CTD; 9442; -.
DR   ZFIN; ZDB-GENE-040426-1601; med27.
DR   eggNOG; ENOG502QS6H; Eukaryota.
DR   GeneTree; ENSGT00390000012207; -.
DR   HOGENOM; CLU_056015_0_0_1; -.
DR   InParanoid; Q6PFL0; -.
DR   OMA; HAAMLHF; -.
DR   OrthoDB; 1256412at2759; -.
DR   PhylomeDB; Q6PFL0; -.
DR   TreeFam; TF323728; -.
DR   Reactome; R-DRE-212436; Generic Transcription Pathway.
DR   PRO; PR:Q6PFL0; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 8.
DR   Bgee; ENSDARG00000009681; Expressed in blastula and 21 other tissues.
DR   ExpressionAtlas; Q6PFL0; baseline.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR   GO; GO:0043010; P:camera-type eye development; IMP:ZFIN.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0046549; P:retinal cone cell development; IMP:ZFIN.
DR   InterPro; IPR021627; Mediator_Med27.
DR   PANTHER; PTHR13130; PTHR13130; 1.
DR   Pfam; PF11571; Med27; 1.
PE   2: Evidence at transcript level;
KW   Activator; Developmental protein; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..311
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   27"
FT                   /id="PRO_0000305012"
SQ   SEQUENCE   311 AA;  35672 MW;  F3EDEA32D8DA25FB CRC64;
     MADVMNVGVN LEAFSQAINA IQALRSSVTR VFDFLKDGMK NKETLESREK VFITEFQENL
     QSVNRDLNEL ERLSTLVGRP SESHPLHNSG LLSLDPVQDK TPLYSQLLQA YKWSNKLQYH
     AGLASSLLNQ QSLKRSANQM GTSAKRRPKV QPSTLALPTQ YVDDVISRIG RMFPDMSIEL
     FRPNGTSAVL LVTLGKVLKA IVVMRSLFID RTIVRGFHEN IYTEDRKLDI WSKSNYQAFQ
     KVTDHATTAL LHYQLPQMPD VVVRSFMTWL RSYIKLFQTP CQRCGKYLQE GLPPTWRDFR
     TLEAFHDTCR Q