ID   MED27_DICDI             Reviewed;         450 AA.
AC   Q55BX7;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 27;
DE   AltName: Full=Putative mediator complex subunit 27;
DE   AltName: Full=Putative mediator complex subunit 3;
DE   AltName: Full=Putative mediator of RNA polymerase II transcription subunit 3;
GN   Name=med27; Synonyms=med3; ORFNames=DDB_G0271072;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 27 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000005; EAL72886.1; -; Genomic_DNA.
DR   RefSeq; XP_646704.1; XM_641612.1.
DR   AlphaFoldDB; Q55BX7; -.
DR   SMR; Q55BX7; -.
DR   STRING; 44689.DDB0266847; -.
DR   PaxDb; Q55BX7; -.
DR   PRIDE; Q55BX7; -.
DR   EnsemblProtists; EAL72886; EAL72886; DDB_G0271072.
DR   GeneID; 8617678; -.
DR   KEGG; ddi:DDB_G0271072; -.
DR   dictyBase; DDB_G0271072; med27.
DR   eggNOG; ENOG502QS6H; Eukaryota.
DR   HOGENOM; CLU_608944_0_0_1; -.
DR   InParanoid; Q55BX7; -.
DR   OMA; SWIWSFR; -.
DR   PRO; PR:Q55BX7; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; ISS:dictyBase.
DR   InterPro; IPR021627; Mediator_Med27.
DR   PANTHER; PTHR13130; PTHR13130; 1.
DR   Pfam; PF11571; Med27; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..450
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 27"
FT                   /id="PRO_0000388643"
FT   COILED          15..118
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   450 AA;  51864 MW;  88A14293096D1225 CRC64;
     MQVQQPQQIQ QQHLQQQQAQ QHQQQQVQQH QQQQQQQQQQ QQIYQQIVNQ PVNNVHQLPS
     LQQQQLLEQY DELLKQVSLI RSNVSDFFNS FIEDAKQQQT IVNKEEMKQK LKKSMDLVLG
     SISSIDKVSK RISSYSFQIA SSNFSTQEAS WAYSTGIYDN RNIKVQLEIR NENYWRSQTS
     YKSSLASSNL ENKLKDLFPI IFQEDEESSS LSKKRKQISS LTSNSLSTTT ITTTTTPSPL
     FKLPSTPPTL LLSGTISPFY DYFAQIERVI HSIRQESALE IFEIQKQSSG APKGLFVECP
     DVFKCLICFG IPNDRKDCFT IDRISFFGVK ENFDSLWASS KYNIFKKISE NSFEAISYYS
     ANPNGGSILK CILSWIWSFR GLFLEECKGC QDILHLDSPQ YMYLPPSFRT FDNYTPYHPS
     CYQNHLLSCN SFSSSTSSSI QQSPFSPLNK