MED27_DROME
ID MED27_DROME Reviewed; 293 AA.
AC Q9VNG0;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 27;
DE AltName: Full=Mediator complex subunit 27;
DE AltName: Full=dCRSP34;
DE AltName: Full=dMED27;
DE AltName: Full=dTRAP37;
GN Name=MED27; Synonyms=Trap37; ORFNames=CG1245;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=11259581; DOI=10.1128/mcb.21.7.2312-2323.2001;
RA Park J.M., Gim B.S., Kim J.M., Yoon J.H., Kim H.-S., Kang J.-G., Kim Y.-J.;
RT "Drosophila Mediator complex is broadly utilized by diverse gene-specific
RT transcription factors at different types of core promoters.";
RL Mol. Cell. Biol. 21:2312-2323(2001).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND FUNCTION OF THE MEDIATOR COMPLEX.
RX PubMed=12021283; DOI=10.1074/jbc.m204144200;
RA Gu J.-Y., Park J.M., Song E.J., Mizuguchi G., Yoon J.H., Kim-Ha J.,
RA Lee K.-J., Kim Y.-J.;
RT "Novel Mediator proteins of the small Mediator complex in Drosophila SL2
RT cells.";
RL J. Biol. Chem. 277:27154-27161(2002).
RN [6]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. Required for activated
CC transcription of the MtnA gene. {ECO:0000269|PubMed:12021283,
CC ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex, which includes at least
CC CDK8, MED4, MED6, MED11, MED14, MED17, MED18, MED20, MED21, MED22,
CC MED27, MED28, MED30 and MED31. {ECO:0000269|PubMed:12021283}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Maternally encoded. Expression decreases during
CC larval stages then rises during mid-pupal metamorphosis.
CC {ECO:0000269|PubMed:11259581}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 27 family.
CC {ECO:0000305}.
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DR EMBL; AE014297; AAF51976.1; -; Genomic_DNA.
DR EMBL; AY075500; AAL68310.1; -; mRNA.
DR RefSeq; NP_649569.1; NM_141312.4.
DR AlphaFoldDB; Q9VNG0; -.
DR SMR; Q9VNG0; -.
DR BioGRID; 65902; 31.
DR IntAct; Q9VNG0; 2.
DR STRING; 7227.FBpp0078361; -.
DR PaxDb; Q9VNG0; -.
DR PRIDE; Q9VNG0; -.
DR DNASU; 40696; -.
DR EnsemblMetazoa; FBtr0078712; FBpp0078361; FBgn0037359.
DR GeneID; 40696; -.
DR KEGG; dme:Dmel_CG1245; -.
DR CTD; 9442; -.
DR FlyBase; FBgn0037359; MED27.
DR VEuPathDB; VectorBase:FBgn0037359; -.
DR eggNOG; ENOG502QS6H; Eukaryota.
DR GeneTree; ENSGT00390000012207; -.
DR HOGENOM; CLU_056015_0_0_1; -.
DR InParanoid; Q9VNG0; -.
DR OMA; HAAMLHF; -.
DR OrthoDB; 1256412at2759; -.
DR PhylomeDB; Q9VNG0; -.
DR BioGRID-ORCS; 40696; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40696; -.
DR PRO; PR:Q9VNG0; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037359; Expressed in testis and 30 other tissues.
DR Genevisible; Q9VNG0; DM.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR InterPro; IPR021627; Mediator_Med27.
DR PANTHER; PTHR13130; PTHR13130; 2.
DR Pfam; PF11571; Med27; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..293
FT /note="Mediator of RNA polymerase II transcription subunit
FT 27"
FT /id="PRO_0000305018"
SQ SEQUENCE 293 AA; 33868 MW; 926ED4D544BD2443 CRC64;
MDKLNSTLTA VKNLRSNVRL CFEHLADGTD GESGEESRNK FVNDFQERFA AINSQIREVE
QLINGLPVPP TPYSLGNTAY LAQETSQDRQ ALYPQLVNSY KWMDKVHDHS FLAFNNLNQN
TLRRSYNYCS QKRQRLPFSS FNNDPDHIDK LLSEINTPPH TSYRIFRPFG TNAVTIVTIS
NVMKAAIVFK GVLIEWVTVK GFDEPLEHDD LWAESRYEVF RKVQDHAHSA MLHFFSPTLP
DLAVKSYITW LNSHVKLFLE PCKRCGKFVV NGLPPTWRDL RTLEPFHEDC RNC
