ARH40_MOUSE
ID ARH40_MOUSE Reviewed; 1517 AA.
AC Q3UPH7; Q3U1X1; Q3UGT7; Q3UYX3; Q6KAS1; Q6PD04; Q6PDY3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Rho guanine nucleotide exchange factor 40;
DE AltName: Full=Protein SOLO;
GN Name=Arhgef40; Synonyms=Solo;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15449545; DOI=10.1093/dnares/11.2.127;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of FLJ genes: the
RT complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified
RT by screening of terminal sequences of cDNA clones randomly sampled from
RT size-fractionated libraries.";
RL DNA Res. 11:127-135(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-593.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1130-1517 (ISOFORM 6).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16143467; DOI=10.1016/j.gene.2005.06.025;
RA Tse S.-W., Broderick J.A., Wei M.-L., Luo M.-H., Smith D., McCaffery P.,
RA Stamm S., Andreadis A.;
RT "Identification, expression analysis, genomic organization and cellular
RT location of a novel protein with a RhoGEF domain.";
RL Gene 359:63-72(2005).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-959, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May act as a guanine nucleotide exchange factor (GEF).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Note=Concentrated in the
CC perinuclear region. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q3UPH7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3UPH7-2; Sequence=VSP_030387, VSP_030388;
CC Name=3;
CC IsoId=Q3UPH7-3; Sequence=VSP_030387;
CC Name=6;
CC IsoId=Q3UPH7-6; Sequence=VSP_030387, VSP_030389;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed but enriched in brain. In
CC brain, it is expressed at higher level in olfactory bulb and cerebellum
CC and peaks perinatally (from 16 dpc to P0.5).
CC {ECO:0000269|PubMed:16143467}.
CC -!- MISCELLANEOUS: [Isoform 6]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH58405.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAD21386.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAE22088.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
CC Sequence=BAE33372.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AK131136; BAD21386.1; ALT_INIT; mRNA.
DR EMBL; AK134295; BAE22088.1; ALT_SEQ; mRNA.
DR EMBL; AK143527; BAE25418.1; -; mRNA.
DR EMBL; AK147761; BAE28120.1; -; mRNA.
DR EMBL; AK155659; BAE33372.1; ALT_SEQ; mRNA.
DR EMBL; BC058405; AAH58405.1; ALT_INIT; mRNA.
DR EMBL; BC059018; AAH59018.1; -; mRNA.
DR CCDS; CCDS49488.1; -. [Q3UPH7-1]
DR CCDS; CCDS49489.1; -. [Q3UPH7-2]
DR CCDS; CCDS59616.1; -. [Q3UPH7-3]
DR RefSeq; NP_001139393.1; NM_001145921.1. [Q3UPH7-2]
DR RefSeq; NP_001139394.1; NM_001145922.1. [Q3UPH7-3]
DR RefSeq; NP_937892.2; NM_198249.4. [Q3UPH7-1]
DR RefSeq; XP_006519107.1; XM_006519044.3. [Q3UPH7-1]
DR RefSeq; XP_006519109.1; XM_006519046.2. [Q3UPH7-2]
DR AlphaFoldDB; Q3UPH7; -.
DR SMR; Q3UPH7; -.
DR IntAct; Q3UPH7; 2.
DR STRING; 10090.ENSMUSP00000091331; -.
DR iPTMnet; Q3UPH7; -.
DR PhosphoSitePlus; Q3UPH7; -.
DR EPD; Q3UPH7; -.
DR jPOST; Q3UPH7; -.
DR MaxQB; Q3UPH7; -.
DR PaxDb; Q3UPH7; -.
DR PeptideAtlas; Q3UPH7; -.
DR PRIDE; Q3UPH7; -.
DR ProteomicsDB; 283205; -. [Q3UPH7-1]
DR ProteomicsDB; 283206; -. [Q3UPH7-2]
DR ProteomicsDB; 283207; -. [Q3UPH7-3]
DR ProteomicsDB; 283208; -. [Q3UPH7-6]
DR Antibodypedia; 22091; 32 antibodies from 12 providers.
DR DNASU; 268739; -.
DR Ensembl; ENSMUST00000093813; ENSMUSP00000091331; ENSMUSG00000004562. [Q3UPH7-1]
DR Ensembl; ENSMUST00000100639; ENSMUSP00000098204; ENSMUSG00000004562. [Q3UPH7-2]
DR Ensembl; ENSMUST00000182061; ENSMUSP00000138128; ENSMUSG00000004562. [Q3UPH7-3]
DR Ensembl; ENSMUST00000182909; ENSMUSP00000138635; ENSMUSG00000004562. [Q3UPH7-1]
DR GeneID; 268739; -.
DR KEGG; mmu:268739; -.
DR UCSC; uc007tnr.2; mouse. [Q3UPH7-1]
DR UCSC; uc007tns.2; mouse. [Q3UPH7-2]
DR UCSC; uc007tnt.2; mouse. [Q3UPH7-3]
DR CTD; 55701; -.
DR MGI; MGI:2685515; Arhgef40.
DR VEuPathDB; HostDB:ENSMUSG00000004562; -.
DR eggNOG; KOG0689; Eukaryota.
DR GeneTree; ENSGT00940000161599; -.
DR InParanoid; Q3UPH7; -.
DR OMA; CMAGPTG; -.
DR OrthoDB; 578732at2759; -.
DR PhylomeDB; Q3UPH7; -.
DR TreeFam; TF334329; -.
DR Reactome; R-MMU-193648; NRAGE signals death through JNK.
DR Reactome; R-MMU-416482; G alpha (12/13) signalling events.
DR Reactome; R-MMU-8980692; RHOA GTPase cycle.
DR Reactome; R-MMU-9013106; RHOC GTPase cycle.
DR BioGRID-ORCS; 268739; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Arhgef40; mouse.
DR PRO; PR:Q3UPH7; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q3UPH7; protein.
DR Bgee; ENSMUSG00000004562; Expressed in ventricular zone and 207 other tissues.
DR ExpressionAtlas; Q3UPH7; baseline and differential.
DR Genevisible; Q3UPH7; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR Gene3D; 1.20.900.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF48065; SSF48065; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm;
KW Guanine-nucleotide releasing factor; Phosphoprotein; Reference proteome.
FT CHAIN 1..1517
FT /note="Rho guanine nucleotide exchange factor 40"
FT /id="PRO_0000314823"
FT DOMAIN 1083..1251
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT DOMAIN 1263..1370
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 194..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 254..514
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 952..1011
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1464..1517
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 754..792
FT /evidence="ECO:0000255"
FT COMPBIAS 202..216
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 275..290
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 303..318
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 423..473
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 474..505
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 952..971
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 975..993
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1487..1517
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 262
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TER5"
FT MOD_RES 367
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8TER5"
FT MOD_RES 415
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TER5"
FT MOD_RES 929
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TER5"
FT MOD_RES 959
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1080
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TER5"
FT MOD_RES 1431
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TER5"
FT MOD_RES 1436
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TER5"
FT MOD_RES 1472
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TER5"
FT MOD_RES 1490
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q8TER5"
FT VAR_SEQ 1413..1460
FT /note="Missing (in isoform 2, isoform 3 and isoform 6)"
FT /evidence="ECO:0000303|PubMed:15449545,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_030387"
FT VAR_SEQ 1504
FT /note="Q -> QAWLRGIK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15449545"
FT /id="VSP_030388"
FT VAR_SEQ 1505..1517
FT /note="SHSRALSDPTTPL -> AWLRGIKVRDQKQS (in isoform 6)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_030389"
FT CONFLICT 381
FT /note="A -> T (in Ref. 1; BAD21386 and 2; BAE33372)"
FT /evidence="ECO:0000305"
FT CONFLICT 392
FT /note="E -> K (in Ref. 2; BAE22088)"
FT /evidence="ECO:0000305"
FT CONFLICT 501
FT /note="N -> D (in Ref. 1; BAD21386 and 2; BAE33372)"
FT /evidence="ECO:0000305"
FT CONFLICT 862
FT /note="A -> V (in Ref. 1; BAD21386)"
FT /evidence="ECO:0000305"
FT CONFLICT 1108
FT /note="L -> P (in Ref. 1; BAD21386)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1517 AA; 165132 MW; 63A0F5900AC42D2E CRC64;
MEPEPVEDCV QSTLAALYPP FEATAPTLLG QVFQVVERTY QEDALRYTLD FLVPAKHLLA
KVQQEACAQY SGFLFFHEGW PLCLHEQVVV QLAALPWQLL RPGDFYLQVV PSAAQAPRLA
LKCLAPGGGR VQELPVPSEA CAYLFTPEWL QGINKDRPTG RLSTCLLSAP SGIQRLPWAE
LVCPRFVHKE GLMVGHQPST LPPELPSGPP GLPSSPLPEE VLGTRSPGDG HNAPAEGPEG
EYVELLEVTL PVRGSPVDAE ASGLSRTRTV PARKSTGGKG RHRRHRAWMN QKGLGSRDQD
GTRPPGEGSS TGASSDSPSG AEADPDATAL QASEPPAEAL GEAPESCLLS GEAVGGVGQG
AEGPPGTPRR TGKGNRRKKR AAGRGAGSRG GEGTSLSPRD KEETRQQEVL VSLPSPSEQE
PAECSLVKEK EDSGKQESES KEELKPADEK EPARPEDYEP PEEEIRESEK EELTPQCTAG
STGPEWFPSE PSTQPLETVQ NVKGDSLPEE TPPVSVLDDP VVAWDLMASG FFVLTGGVDQ
TGRALLTITP PPPCLPEESS PSQETLSTAL RYLHSLLRPD LQLLGLTILL DLRKAPPLPP
ALLPALSQLQ DSGEPPLIQR LVILIHEDPP AELCGFQGAE LLSEKDLKRV AKPEELQWDL
GGHRDLSPNH WAEIHQEVAR LCTLCQGVLT SVRQAIEELE GTAEPKEEEA VGMPEPLQKV
LADPRLTALQ RNGGAILMRL RSAHSSKLEG PGPAELYQEV DEAIHQLVRL SNLRVQQQEE
RQRLRQVQQV LQWLSGPGEE QLASFSMPGN SLSVLQETEL RFRAFSTEVQ ERLVQAREAL
ALEEDLTSQK VLDIFEQRLE QAESGLHRAL RLQRFFQQAH EWVDEGSARL AGAGSGREAL
LAALALRRAP EPSAGTFQEM RALALDLGSP AALREWGRCR ARCQELERRI QQQLGEEASP
RSHRRRRADS ASSAGAQHGA HSPSPSLSSL LLPSSPGPRA APSHCSLTPC GEDYEEEGLE
LAPETDGRPP RAVLIRGLEV TSTEVVDRTC SPREHVLLGR AGGPDGPWGI GTPRMERKRS
ISAQQRLVSE LIACEQEYVT TLNEPVPLPG PELTPELRCT WAAALSVRER LRSFHGTHFL
QELQGCAAHP LRIGACFLRH GDQFNLYAQF VKHRHKLESG LAALTPSVKG SMESSPCLPR
ALQQPLEQLA RYGQLLEELL REAGPELSSE RQALRAAVQL LQEQEARGRD LLAVEAVRGC
EIDLKEQGQL LHRDPFTVIC GRKKCLRHVF LFEDLLLFSK LKGSEGGSET FVYKQAFKTA
DMGLTENIGD SGLCFELWFR RRRAREAYTL QATSPETKLK WTSSIAQLLW RQAAHNKELR
VQQMVSMGIG NKPFLDIKAL GERTLSALLT GRAARTRASV AVSSFEHAGP SLPGLSPGAC
SLPARVEEEA WDLDVKQISL ASETLDSSGD VSPGPRNSPS LQPPSPGSST PALTSGGILG
LSRQSHSRAL SDPTTPL
