MED27_MOUSE
ID MED27_MOUSE Reviewed; 311 AA.
AC Q9DB40; A2AJV2; Q78JI3; Q9D707;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 27;
DE AltName: Full=Cofactor required for Sp1 transcriptional activation subunit 8;
DE Short=CRSP complex subunit 8;
DE AltName: Full=Mediator complex subunit 27;
GN Name=Med27; Synonyms=Crsp8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:BAB26488.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J;
RC TISSUE=Cerebellum, Heart {ECO:0000312|EMBL:BAE27743.1}, and
RC Tongue {ECO:0000312|EMBL:BAB26488.2};
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAH16537.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N {ECO:0000312|EMBL:AAH16537.1};
RC TISSUE=Mammary gland {ECO:0000312|EMBL:AAH16537.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000305};
CC IsoId=Q9DB40-1; Sequence=Displayed;
CC Name=2 {ECO:0000305};
CC IsoId=Q9DB40-2; Sequence=VSP_051870, VSP_051871;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 27 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH16537.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK005251; BAB23906.2; -; mRNA.
DR EMBL; AK009764; BAB26488.2; -; mRNA.
DR EMBL; AK147181; BAE27743.1; -; mRNA.
DR EMBL; AL772213; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL929437; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC016537; AAH16537.1; ALT_INIT; mRNA.
DR CCDS; CCDS15853.1; -. [Q9DB40-1]
DR CCDS; CCDS71014.1; -. [Q9DB40-2]
DR RefSeq; NP_001277419.1; NM_001290490.1.
DR RefSeq; NP_081172.2; NM_026896.5. [Q9DB40-1]
DR RefSeq; NP_083891.1; NM_029615.1. [Q9DB40-2]
DR PDB; 6W1S; EM; 4.02 A; V=8-304.
DR PDBsum; 6W1S; -.
DR AlphaFoldDB; Q9DB40; -.
DR SMR; Q9DB40; -.
DR BioGRID; 213151; 2.
DR ComplexPortal; CPX-3264; Core mediator complex.
DR IntAct; Q9DB40; 3.
DR MINT; Q9DB40; -.
DR STRING; 10090.ENSMUSP00000028139; -.
DR iPTMnet; Q9DB40; -.
DR PhosphoSitePlus; Q9DB40; -.
DR EPD; Q9DB40; -.
DR MaxQB; Q9DB40; -.
DR PaxDb; Q9DB40; -.
DR PeptideAtlas; Q9DB40; -.
DR PRIDE; Q9DB40; -.
DR ProteomicsDB; 295989; -. [Q9DB40-1]
DR ProteomicsDB; 295990; -. [Q9DB40-2]
DR Antibodypedia; 1810; 162 antibodies from 27 providers.
DR DNASU; 68975; -.
DR Ensembl; ENSMUST00000028139; ENSMUSP00000028139; ENSMUSG00000026799. [Q9DB40-1]
DR Ensembl; ENSMUST00000113830; ENSMUSP00000109461; ENSMUSG00000026799. [Q9DB40-2]
DR GeneID; 68975; -.
DR KEGG; mmu:68975; -.
DR UCSC; uc008izr.2; mouse. [Q9DB40-2]
DR UCSC; uc008izs.2; mouse. [Q9DB40-1]
DR CTD; 9442; -.
DR MGI; MGI:1916225; Med27.
DR VEuPathDB; HostDB:ENSMUSG00000026799; -.
DR eggNOG; ENOG502QS6H; Eukaryota.
DR GeneTree; ENSGT00390000012207; -.
DR HOGENOM; CLU_056015_0_0_1; -.
DR InParanoid; Q9DB40; -.
DR OMA; HAAMLHF; -.
DR OrthoDB; 1256412at2759; -.
DR PhylomeDB; Q9DB40; -.
DR TreeFam; TF323728; -.
DR Reactome; R-MMU-212436; Generic Transcription Pathway.
DR BioGRID-ORCS; 68975; 17 hits in 72 CRISPR screens.
DR ChiTaRS; Med27; mouse.
DR PRO; PR:Q9DB40; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9DB40; protein.
DR Bgee; ENSMUSG00000026799; Expressed in animal zygote and 233 other tissues.
DR ExpressionAtlas; Q9DB40; baseline and differential.
DR Genevisible; Q9DB40; MM.
DR GO; GO:0070847; C:core mediator complex; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; ISO:MGI.
DR GO; GO:0000151; C:ubiquitin ligase complex; ISO:MGI.
DR GO; GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; ISO:MGI.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0016567; P:protein ubiquitination; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR GO; GO:0035019; P:somatic stem cell population maintenance; IMP:MGI.
DR InterPro; IPR021627; Mediator_Med27.
DR PANTHER; PTHR13130; PTHR13130; 1.
DR Pfam; PF11571; Med27; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Alternative splicing; Methylation; Nucleus;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..311
FT /note="Mediator of RNA polymerase II transcription subunit
FT 27"
FT /id="PRO_0000079362"
FT MOD_RES 132
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6P2C8"
FT MOD_RES 134
FT /note="N6-methyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q6P2C8"
FT VAR_SEQ 192..208
FT /note="VTLGKVLKVIVVMRSLF -> ILINSSAQSITGPWFWS (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_051870"
FT VAR_SEQ 209..311
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_051871"
SQ SEQUENCE 311 AA; 35298 MW; 60E208A64A2BA6BF CRC64;
MADVLSVGVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIANFQDNL
HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK TPLYSQLLQA YKWSNKLQYH
AGLASGLLNQ QSLKRSANQM GVSAKRRPKA QPTTLVLPPQ YVDDVISRID RMFPEMSIHL
SRPNGTSAML LVTLGKVLKV IVVMRSLFID RTIVKGYNES VYTEDGKLDI WSKSSYQVFQ
KVTDHATTAL LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR
TLEAFHDTCR Q
