MED27_PIG
ID MED27_PIG Reviewed; 311 AA.
AC Q6Q7J5;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 27;
DE AltName: Full=Cofactor required for Sp1 transcriptional activation subunit 8;
DE Short=CRSP complex subunit 8;
DE AltName: Full=Mediator complex subunit 27;
GN Name=MED27; Synonyms=CRSP8;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Lee H.Y., Cui X.S., Shin M.L., Jeong Y.J., Hwang K.C., Kim N.H.;
RT "Identification of differentially expressed genes in porcine embryos.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 27 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY553926; AAS76547.1; -; mRNA.
DR RefSeq; NP_001001644.1; NM_001001644.1.
DR AlphaFoldDB; Q6Q7J5; -.
DR SMR; Q6Q7J5; -.
DR STRING; 9823.ENSSSCP00000006128; -.
DR PaxDb; Q6Q7J5; -.
DR PRIDE; Q6Q7J5; -.
DR Ensembl; ENSSSCT00000053035; ENSSSCP00000034741; ENSSSCG00000005720.
DR Ensembl; ENSSSCT00005007957; ENSSSCP00005004625; ENSSSCG00005005142.
DR Ensembl; ENSSSCT00015085158; ENSSSCP00015034599; ENSSSCG00015063468.
DR Ensembl; ENSSSCT00050017916; ENSSSCP00050007395; ENSSSCG00050013272.
DR Ensembl; ENSSSCT00055012870; ENSSSCP00055010123; ENSSSCG00055006660.
DR Ensembl; ENSSSCT00070013469; ENSSSCP00070011104; ENSSSCG00070006990.
DR GeneID; 414430; -.
DR KEGG; ssc:414430; -.
DR CTD; 9442; -.
DR VGNC; VGNC:90117; MED27.
DR eggNOG; ENOG502QS6H; Eukaryota.
DR GeneTree; ENSGT00390000012207; -.
DR HOGENOM; CLU_056015_0_0_1; -.
DR InParanoid; Q6Q7J5; -.
DR OMA; HAAMLHF; -.
DR OrthoDB; 1256412at2759; -.
DR TreeFam; TF323728; -.
DR Reactome; R-SSC-212436; Generic Transcription Pathway.
DR ChiTaRS; MED27; pig.
DR Proteomes; UP000008227; Chromosome 1.
DR Proteomes; UP000314985; Chromosome 1.
DR Bgee; ENSSSCG00000005720; Expressed in oocyte and 43 other tissues.
DR ExpressionAtlas; Q6Q7J5; baseline and differential.
DR Genevisible; Q6Q7J5; SS.
DR GO; GO:0070847; C:core mediator complex; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0035019; P:somatic stem cell population maintenance; IEA:Ensembl.
DR InterPro; IPR021627; Mediator_Med27.
DR PANTHER; PTHR13130; PTHR13130; 1.
DR Pfam; PF11571; Med27; 1.
PE 2: Evidence at transcript level;
KW Activator; Methylation; Nucleus; Phosphoprotein; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..311
FT /note="Mediator of RNA polymerase II transcription subunit
FT 27"
FT /id="PRO_0000293486"
FT MOD_RES 132
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6P2C8"
FT MOD_RES 134
FT /note="N6-methyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q6P2C8"
SQ SEQUENCE 311 AA; 35375 MW; 5E8DE8BA9F3A5E68 CRC64;
MADVLSVGVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL
HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK TPLYSQLLQA YKWSNKLQYH
AGLASGLLNQ QSLKRSANQM GVSAKRRPKA QPTTLVLPPQ YVDDVISRID RMFPEMSIHL
SRPNGTSAML LVTLGKVLKV IVVMRSLFID RTIVKGYNEN VYTEDGKLDI WSKSNYQVFQ
KVTDHATTAL LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR
TLEAFHDTCR Q