ID   MED27_PONAB             Reviewed;         311 AA.
AC   Q5R6U8;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 27;
DE   AltName: Full=Cofactor required for Sp1 transcriptional activation subunit 8;
DE            Short=CRSP complex subunit 8;
DE   AltName: Full=Mediator complex subunit 27;
GN   Name=MED27; Synonyms=CRSP8;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain cortex;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC       MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC       MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC       MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC       CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC       module termed the CDK8 module. Mediator containing the CDK8 module is
CC       less active than Mediator lacking this module in supporting
CC       transcriptional activation. Individual preparations of the Mediator
CC       complex lacking one or more distinct subunits have been variously
CC       termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 27 family.
CC       {ECO:0000305}.
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DR   EMBL; CR860386; CAH92512.1; -; mRNA.
DR   RefSeq; NP_001127559.1; NM_001134087.1.
DR   AlphaFoldDB; Q5R6U8; -.
DR   SMR; Q5R6U8; -.
DR   STRING; 9601.ENSPPYP00000022097; -.
DR   Ensembl; ENSPPYT00000023006; ENSPPYP00000022097; ENSPPYG00000019706.
DR   GeneID; 100174637; -.
DR   KEGG; pon:100174637; -.
DR   CTD; 9442; -.
DR   eggNOG; ENOG502QS6H; Eukaryota.
DR   GeneTree; ENSGT00390000012207; -.
DR   HOGENOM; CLU_056015_0_0_1; -.
DR   InParanoid; Q5R6U8; -.
DR   OMA; HAAMLHF; -.
DR   OrthoDB; 1256412at2759; -.
DR   TreeFam; TF323728; -.
DR   Proteomes; UP000001595; Chromosome 9.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   InterPro; IPR021627; Mediator_Med27.
DR   PANTHER; PTHR13130; PTHR13130; 1.
DR   Pfam; PF11571; Med27; 1.
PE   2: Evidence at transcript level;
KW   Activator; Methylation; Nucleus; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..311
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   27"
FT                   /id="PRO_0000293487"
FT   MOD_RES         132
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6P2C8"
FT   MOD_RES         134
FT                   /note="N6-methyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q6P2C8"
SQ   SEQUENCE   311 AA;  35432 MW;  996D675993E393CB CRC64;
     MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL
     HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK TPLYSQLLQA YKWSNKLQYH
     AGLASGLLNQ QSLKRSANQM GVSAKRRPKA QPTTLVLPPQ YVDDVISRID RMFPEMSIHL
     SRPNGTSAML LVTLGKVLKV IVVMRSLFID RTIVKGYNEN VYTEDGKLDI WSKSNYQVFQ
     KVTDHATTAL LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR
     TLEAFHDTCR Q