ID   MED27_SCHPO             Reviewed;         273 AA.
AC   Q10477;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 27;
DE   AltName: Full=Mediator complex subunit 27;
DE   AltName: Full=RNA polymerase II mediator complex protein pmc3;
GN   Name=med27; Synonyms=pmc3; ORFNames=SPAC17C9.05c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP   COMPLEX.
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=10625684; DOI=10.1074/jbc.275.2.1351;
RA   Spaehr H., Beve J., Larsson T., Bergstroem J., Karlsson K.-A.,
RA   Gustafsson C.M.;
RT   "Purification and characterization of RNA polymerase II holoenzyme from
RT   Schizosaccharomyces pombe.";
RL   J. Biol. Chem. 275:1351-1356(2000).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP   COMPLEX.
RX   PubMed=11572939; DOI=10.1073/pnas.211253898;
RA   Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA   Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT   "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT   subunits conserved between yeast and metazoan cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors.
CC   -!- SUBUNIT: Component of the Mediator complex.
CC       {ECO:0000269|PubMed:10625684, ECO:0000269|PubMed:11572939}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 27 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CU329670; CAA97351.1; -; Genomic_DNA.
DR   PIR; T11585; T11585.
DR   RefSeq; NP_594601.1; NM_001020029.2.
DR   PDB; 5U0P; EM; 4.40 A; 2=1-273.
DR   PDB; 5U0S; EM; 7.80 A; 2=1-273.
DR   PDBsum; 5U0P; -.
DR   PDBsum; 5U0S; -.
DR   AlphaFoldDB; Q10477; -.
DR   SMR; Q10477; -.
DR   BioGRID; 278617; 32.
DR   IntAct; Q10477; 2.
DR   STRING; 4896.SPAC17C9.05c.1; -.
DR   MaxQB; Q10477; -.
DR   PaxDb; Q10477; -.
DR   EnsemblFungi; SPAC17C9.05c.1; SPAC17C9.05c.1:pep; SPAC17C9.05c.
DR   GeneID; 2542141; -.
DR   KEGG; spo:SPAC17C9.05c; -.
DR   PomBase; SPAC17C9.05c; -.
DR   VEuPathDB; FungiDB:SPAC17C9.05c; -.
DR   HOGENOM; CLU_1082430_0_0_1; -.
DR   InParanoid; Q10477; -.
DR   OMA; FHYECTL; -.
DR   PRO; PR:Q10477; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0003713; F:transcription coactivator activity; IDA:PomBase.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; EXP:PomBase.
DR   InterPro; IPR021627; Mediator_Med27.
DR   Pfam; PF11571; Med27; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..273
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   27"
FT                   /id="PRO_0000096380"
SQ   SEQUENCE   273 AA;  31093 MW;  C88A085C6BFF702C CRC64;
     MSLEEQRTRD ELKHKLLDLN QLHEQLAELR TICPSLLKLL HPETGTSRKF EKSAQEAIEK
     VNSFYTHLKS SQNVFDYAEK SLQADSSNLL PTYLYNSEDL SNDTENNETK SINGKSALDL
     KEPHHSELHD NDNFQNSDIN IESFKGDIEA SGSILTTHEN KSFTLKLANE LEFIFFHDTR
     GKFSVYCSSS KDDAITFSIN RNNNFLGNLW SLMPKILDYQ HLYSKPCDFC KSLISPVYLE
     LPSVRRNANS TVKPTSKDIL ALHAECVPAQ SDL