ID   MED28_BOVIN             Reviewed;         178 AA.
AC   Q2TBN4;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 28;
DE   AltName: Full=Mediator complex subunit 28;
GN   Name=MED28;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors. May be part of a complex
CC       containing NF2/merlin that participates in cellular signaling to the
CC       actin cytoskeleton downstream of tyrosine kinase signaling pathways (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC       MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC       MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC       MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC       CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC       module termed the CDK8 module. Mediator containing the CDK8 module is
CC       less active than Mediator lacking this module in supporting
CC       transcriptional activation. Individual preparations of the Mediator
CC       complex lacking one or more distinct subunits have been variously
CC       termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex
CC       with NF2/merlin and GRB2. Binds to actin (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC       Membrane {ECO:0000250}. Note=May be also cytoplasmic and membrane-
CC       associated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 28 family.
CC       {ECO:0000305}.
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DR   EMBL; BC109886; AAI09887.1; -; mRNA.
DR   RefSeq; NP_001033627.1; NM_001038538.1.
DR   AlphaFoldDB; Q2TBN4; -.
DR   SMR; Q2TBN4; -.
DR   STRING; 9913.ENSBTAP00000025895; -.
DR   PaxDb; Q2TBN4; -.
DR   GeneID; 513972; -.
DR   KEGG; bta:513972; -.
DR   CTD; 80306; -.
DR   eggNOG; ENOG502QSN9; Eukaryota.
DR   InParanoid; Q2TBN4; -.
DR   OrthoDB; 1504193at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   InterPro; IPR021640; Mediator_Med28.
DR   PANTHER; PTHR13512; PTHR13512; 1.
PE   2: Evidence at transcript level;
KW   Actin-binding; Activator; Coiled coil; Cytoplasm; Membrane; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..178
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   28"
FT                   /id="PRO_0000246168"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          109..145
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   178 AA;  19764 MW;  212F3AF5F47956EE CRC64;
     MAAPLGGMFS GQQPGPPQPP PGLLGQASLL QATPGVPRTS NSTLVDELES SFEACFASLV
     SQDYVNGTDQ EEIRTGVDQC IKKFLDIARQ TECFFLQKRL QLSVQKPEQV IKEDVSELRN
     ELQRKDALVQ KHLTKLRHWQ QVLEDINMQH KKPADIPQGS LAYLEQASAN IPAPMKQT