ID   MED28_DANRE             Reviewed;         179 AA.
AC   Q5RKN3;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 28;
DE   AltName: Full=Mediator complex subunit 28;
GN   Name=med28; ORFNames=zgc:103633;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Larva;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 28 family.
CC       {ECO:0000305}.
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DR   EMBL; BC085569; AAH85569.1; -; mRNA.
DR   RefSeq; NP_001007771.1; NM_001007770.1.
DR   AlphaFoldDB; Q5RKN3; -.
DR   SMR; Q5RKN3; -.
DR   STRING; 7955.ENSDARP00000054441; -.
DR   PaxDb; Q5RKN3; -.
DR   GeneID; 493610; -.
DR   KEGG; dre:493610; -.
DR   CTD; 80306; -.
DR   ZFIN; ZDB-GENE-041121-4; med28.
DR   eggNOG; ENOG502QSN9; Eukaryota.
DR   InParanoid; Q5RKN3; -.
DR   OrthoDB; 1504193at2759; -.
DR   PhylomeDB; Q5RKN3; -.
DR   PRO; PR:Q5RKN3; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   InterPro; IPR021640; Mediator_Med28.
DR   PANTHER; PTHR13512; PTHR13512; 1.
PE   2: Evidence at transcript level;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..179
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   28"
FT                   /id="PRO_0000305696"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          109..139
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        13..27
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   179 AA;  19697 MW;  C79D8F5DB19D47A7 CRC64;
     MASSMCGMFP GQQPPGSLPP PGPGGPGQPG LLTGTPGNRG ANNTLVDELE ASFEACFASL
     VSQDYVNGTD QEEIRTGVDQ CIQKFLDVAR QTECFFLQKR LQLSVQKPEQ VEKEDASELK
     NELQRKEMLI QKHLAKIHHW QQVLEDINVQ HKKPTELPQG PLAFLEQASA NLPAPMKPN