ID   MED28_DICDI             Reviewed;         179 AA.
AC   Q54DD4;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 28;
DE   AltName: Full=Putative mediator complex subunit 28;
GN   Name=med28; ORFNames=DDB_G0292324;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 28 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000189; EAL61286.1; -; Genomic_DNA.
DR   RefSeq; XP_629707.1; XM_629705.1.
DR   AlphaFoldDB; Q54DD4; -.
DR   SMR; Q54DD4; -.
DR   STRING; 44689.DDB0266950; -.
DR   PaxDb; Q54DD4; -.
DR   EnsemblProtists; EAL61286; EAL61286; DDB_G0292324.
DR   GeneID; 8628621; -.
DR   KEGG; ddi:DDB_G0292324; -.
DR   dictyBase; DDB_G0292324; med28.
DR   eggNOG; ENOG502RHH4; Eukaryota.
DR   HOGENOM; CLU_1506118_0_0_1; -.
DR   InParanoid; Q54DD4; -.
DR   OMA; VKEWKSH; -.
DR   PhylomeDB; Q54DD4; -.
DR   PRO; PR:Q54DD4; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0016592; C:mediator complex; ISS:dictyBase.
DR   GO; GO:0003712; F:transcription coregulator activity; IC:dictyBase.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IC:dictyBase.
DR   InterPro; IPR021640; Mediator_Med28.
DR   PANTHER; PTHR13512; PTHR13512; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..179
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 28"
FT                   /id="PRO_0000388662"
FT   COILED          81..119
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   179 AA;  20656 MW;  30069CEFFDE0EC63 CRC64;
     MDTNNTNNIN SLENPIDLEN DPNILFTELN VKLESLITFL TKNDKDNQIF VDEIIDNKVT
     DLLIASKELE NYFISQQSKY SAEKNKIQLK QEIYKVKKEI ENKDRLIERY KNKVKEWKYH
     FEPLYQSQNH ILHSTAQGLS GIENQFSPVF TPMPNTPSIL QNLSQAKPSP SLGLGSTIL