MED28_DROME
ID MED28_DROME Reviewed; 189 AA.
AC Q9VBQ9;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 28;
DE AltName: Full=Mediator complex subunit 28;
DE AltName: Full=dMED23;
GN Name=MED28; Synonyms=Med23; ORFNames=CG5121;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND FUNCTION OF THE MEDIATOR COMPLEX.
RX PubMed=12021283; DOI=10.1074/jbc.m204144200;
RA Gu J.-Y., Park J.M., Song E.J., Mizuguchi G., Yoon J.H., Kim-Ha J.,
RA Lee K.-J., Kim Y.-J.;
RT "Novel Mediator proteins of the small Mediator complex in Drosophila SL2
RT cells.";
RL J. Biol. Chem. 277:27154-27161(2002).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. {ECO:0000269|PubMed:12021283}.
CC -!- SUBUNIT: Component of the Mediator complex, which includes at least
CC CDK8, MED4, MED6, MED11, MED14, MED17, MED18, MED20, MED21, MED22,
CC MED27, MED28, MED30 and MED31. {ECO:0000269|PubMed:12021283}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 28 family.
CC {ECO:0000305}.
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DR EMBL; AE014297; AAF56470.1; -; Genomic_DNA.
DR EMBL; AY118876; AAM50736.1; -; mRNA.
DR RefSeq; NP_651397.1; NM_143140.1.
DR AlphaFoldDB; Q9VBQ9; -.
DR SMR; Q9VBQ9; -.
DR BioGRID; 67993; 12.
DR DIP; DIP-19819N; -.
DR IntAct; Q9VBQ9; 8.
DR STRING; 7227.FBpp0084211; -.
DR PaxDb; Q9VBQ9; -.
DR DNASU; 43079; -.
DR EnsemblMetazoa; FBtr0084836; FBpp0084211; FBgn0039337.
DR GeneID; 43079; -.
DR KEGG; dme:Dmel_CG5121; -.
DR CTD; 80306; -.
DR FlyBase; FBgn0039337; MED28.
DR VEuPathDB; VectorBase:FBgn0039337; -.
DR eggNOG; ENOG502QSN9; Eukaryota.
DR GeneTree; ENSGT00390000006192; -.
DR HOGENOM; CLU_088358_0_0_1; -.
DR InParanoid; Q9VBQ9; -.
DR OMA; MQPSPQH; -.
DR OrthoDB; 1504193at2759; -.
DR PhylomeDB; Q9VBQ9; -.
DR BioGRID-ORCS; 43079; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 43079; -.
DR PRO; PR:Q9VBQ9; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039337; Expressed in ovary and 29 other tissues.
DR Genevisible; Q9VBQ9; DM.
DR GO; GO:0016592; C:mediator complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003712; F:transcription coregulator activity; IPI:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IPI:UniProtKB.
DR InterPro; IPR021640; Mediator_Med28.
DR PANTHER; PTHR13512; PTHR13512; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..189
FT /note="Mediator of RNA polymerase II transcription subunit
FT 28"
FT /id="PRO_0000113984"
FT REGION 124..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 75..115
FT /evidence="ECO:0000255"
SQ SEQUENCE 189 AA; 21079 MW; 1F4AE8D49A011C64 CRC64;
MASNESGGGN LMDEFEEAFQ SCLLTLTKQE PNSGTNKEEI DLEVQKTTNR FIDVARQMEA
FFLQKRFLVS TLKPYMLIKD ENQDLSIEIQ RKEALLQKHY NRLEEWKACL SDIQQGVHSR
PTPPIGSGML QGPGGGMPPM GGTPPRPGMM PGMPPGAMQP GGPMQPSPHM LQAQQMQQLR
MISRQMPPK
