ID   MED28_DROPS             Reviewed;         190 AA.
AC   Q294G7;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 28;
DE   AltName: Full=Mediator complex subunit 28;
GN   Name=MED28; ORFNames=GA18674;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 28 family.
CC       {ECO:0000305}.
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DR   EMBL; CM000070; EAL28997.1; -; Genomic_DNA.
DR   RefSeq; XP_001359845.1; XM_001359808.3.
DR   AlphaFoldDB; Q294G7; -.
DR   SMR; Q294G7; -.
DR   STRING; 7237.FBpp0281138; -.
DR   EnsemblMetazoa; FBtr0282700; FBpp0281138; FBgn0078674.
DR   GeneID; 4803044; -.
DR   KEGG; dpo:Dpse_GA18674; -.
DR   eggNOG; ENOG502QSN9; Eukaryota.
DR   HOGENOM; CLU_088358_0_0_1; -.
DR   InParanoid; Q294G7; -.
DR   OMA; MQPSPQH; -.
DR   PhylomeDB; Q294G7; -.
DR   Proteomes; UP000001819; Chromosome 2.
DR   Bgee; FBgn0078674; Expressed in female reproductive system and 2 other tissues.
DR   GO; GO:0016592; C:mediator complex; ISS:UniProtKB.
DR   GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   InterPro; IPR021640; Mediator_Med28.
DR   PANTHER; PTHR13512; PTHR13512; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..190
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   28"
FT                   /id="PRO_0000305699"
FT   COILED          76..108
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   190 AA;  21309 MW;  E2BF1B41B44581F8 CRC64;
     MAQNDPGSGN LMDEFEEAFQ SCLLSLTKQE PNSGTNKEEI ELEVQKTTNR FIDVARQMEA
     FFLQKRFLVS TLKPYMLIKD ENQDLSIEIQ RKEALLQKHY NRLEEWKACL SDIQQGVHNR
     PTPPINPGML QGPSGMQQPM GGGVPPRPGL MPGMPPGGMS PVGPMPPGQQ HMLQAQQMQQ
     LRMMGKLPPK